Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt12m08
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 227 8e-61
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 227 8e-61
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 227 8e-61
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 77 2e-15
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 42 1e-04
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 27 3.1
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 5.5
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 7.2
SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 25 9.5
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc... 25 9.5
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 227 bits (556), Expect = 8e-61
Identities = 103/134 (76%), Positives = 118/134 (88%)
Frame = -3
Query: 651 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 472
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 471 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 292
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 291 DMRQTVAVGVIKAV 250
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 227 bits (556), Expect = 8e-61
Identities = 103/134 (76%), Positives = 118/134 (88%)
Frame = -3
Query: 651 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 472
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 471 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 292
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 291 DMRQTVAVGVIKAV 250
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 227 bits (556), Expect = 8e-61
Identities = 103/134 (76%), Positives = 118/134 (88%)
Frame = -3
Query: 651 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 472
VGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAH
Sbjct: 306 VGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAH 365
Query: 471 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 292
IACKFAE+ EK+DRR+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVR
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVR 425
Query: 291 DMRQTVAVGVIKAV 250
DMRQTVAVGVIKAV
Sbjct: 426 DMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 77.0 bits (181), Expect = 2e-15
Identities = 42/125 (33%), Positives = 69/125 (55%), Gaps = 1/125 (0%)
Frame = -3
Query: 618 ELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEK 439
+++ GYV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K
Sbjct: 540 DVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHK 598
Query: 438 VDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-V 262
+D+ T + ++ P G I L P+C+E F+++ +GRF +RD TVAVG V
Sbjct: 599 LDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKV 657
Query: 261 IKAVN 247
+K ++
Sbjct: 658 VKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 41.5 bits (93), Expect = 1e-04
Identities = 37/132 (28%), Positives = 59/132 (44%)
Frame = -3
Query: 651 VGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAH 472
V + ++ V +LR G + + +N P + F A++ + G I +G T VL H
Sbjct: 467 VTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----H 520
Query: 471 IACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVR 292
+ + K+ K + + S K I L PLC+ +E P LGRF +R
Sbjct: 521 LGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILR 577
Query: 291 DMRQTVAVGVIK 256
TVA G++K
Sbjct: 578 RSGDTVAAGIVK 589
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 26.6 bits (56), Expect = 3.1
Identities = 14/34 (41%), Positives = 15/34 (44%)
Frame = -2
Query: 124 GVSKEKRATNSFLFYIFYKACNVTLFYNLYKVIP 23
G S E S+ IFY FYNL KV P
Sbjct: 762 GASSEMECYFSYCSLIFYYQATTLQFYNLPKVRP 795
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 5.5
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 206 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 379
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 380 SPDLMDFGLTSVDLPVRRSTFSLIS 454
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 371 MAASPDLMDFGLTSVDLPVRRSTFS 445
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
>SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +2
Query: 191 PLVAFSAALVTLPPPASLKLTALMTPTATVCLMSRTA 301
P + ++ T+PP S+ T + PT ++ + TA
Sbjct: 73 PSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTA 109
>SPBC18H10.04c |sce3|tif48|translation initiation factor
eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
Length = 388
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +2
Query: 218 VTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDST 340
+ L P +S + TP+AT S+ + P GG D+T
Sbjct: 244 LNLKPRSSSNVNTEATPSATTTTSSKPKRDPFGGAKPVDNT 284
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,667,252
Number of Sequences: 5004
Number of extensions: 53282
Number of successful extensions: 143
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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