Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt12f05
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 91 1e-19
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 85 1e-17
SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 68 1e-12
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 46 5e-06
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.25
SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr 1|... 26 4.1
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 5.5
SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha... 25 9.5
SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family |Schizosa... 25 9.5
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 25 9.5
SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc... 25 9.5
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 9.5
>SPBC16E9.09c |||COPII vesicle coat component
Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 215
Score = 91.1 bits (216), Expect = 1e-19
Identities = 54/159 (33%), Positives = 86/159 (54%), Gaps = 4/159 (2%)
Frame = -2
Query: 586 TTPGIGMHVEVRDP--NDRILLSRVYSLEGMISFTSSTPGEHVICMYSNST-SWFSGSQL 416
T+ I + V+V + N+ ++ + FT++ G H IC S S +WFS ++
Sbjct: 60 TSNNIKIRVQVDETFANNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSSTKT 119
Query: 415 RVHLDIQVGEHAVDYANVAQKE-KLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTS 239
R+H+D+ + Y +A E + +L R+ L ++ I EQ QR RE FR TS
Sbjct: 120 RLHVDLASDDK---YNLIASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTS 176
Query: 238 ESTNQRVLWWSLLQTGVLVIIGYWQMRHLKSFFEAKKLV 122
ES N RV+ W+++Q VL++ WQ+ HL+ FF +KLV
Sbjct: 177 ESANSRVVRWTIVQIVVLLVTCIWQLSHLQRFFVKEKLV 215
>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 199
Score = 84.6 bits (200), Expect = 1e-17
Identities = 48/147 (32%), Positives = 78/147 (53%)
Frame = -2
Query: 562 VEVRDPNDRILLSRVYSLEGMISFTSSTPGEHVICMYSNSTSWFSGSQLRVHLDIQVGEH 383
+ + +P +I+ V + SFT PG+++ C Y+++ G V ++ H
Sbjct: 60 MSIYNPAGQIMHQEVPNSMAQYSFTVKNPGKYMYCFYNDA---LDGESKEVLFNV----H 112
Query: 382 AVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSL 203
V Y + + L ++RQL D + ++ EQ Y RE R T+ESTN RV WWS+
Sbjct: 113 GVIYISDEDLDANNPLLGKVRQLHDTISKVKHEQEYFVARERIHRNTAESTNDRVKWWSI 172
Query: 202 LQTGVLVIIGYWQMRHLKSFFEAKKLV 122
LQT +LV + +Q+ +LK FE K++V
Sbjct: 173 LQTVILVSVCVFQIFYLKRLFEVKRVV 199
>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 216
Score = 67.7 bits (158), Expect = 1e-12
Identities = 35/97 (36%), Positives = 58/97 (59%)
Frame = -2
Query: 412 VHLDIQVGEHAVDYANVAQKEKLTELQLRIRQLLDQVHQITKEQAYQRQREERFRQTSES 233
V L+ VG A DY+++ + L ++ IR+ D + +I + Y + RE RFR T+ES
Sbjct: 119 VKLEFTVGADADDYSSLQKANNLEPVEADIRRARDFIEEIKGKIYYLQAREARFRNTNES 178
Query: 232 TNQRVLWWSLLQTGVLVIIGYWQMRHLKSFFEAKKLV 122
TN+RV ++ L L ++ WQ+ +L+SFF+ K L+
Sbjct: 179 TNERVKNFAYLTFISLFVLVIWQILYLRSFFQRKHLI 215
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 46.0 bits (104), Expect = 5e-06
Identities = 30/123 (24%), Positives = 63/123 (51%), Gaps = 2/123 (1%)
Frame = -2
Query: 499 ISFTSSTPGEHVICMYSNSTSWFSGS--QLRVHLDIQVGEHAVDYANVAQKEKLTELQLR 326
+ FT GE+ C + N S F+ + + ++ ++ A+ + A+ K +Q
Sbjct: 83 VFFTLEEKGEYEFC-FDNHMSTFTDKIVTMEITMENELSLPALT-RDEAKDYKKDSMQST 140
Query: 325 IRQLLDQVHQITKEQAYQRQREERFRQTSESTNQRVLWWSLLQTGVLVIIGYWQMRHLKS 146
+ ++ + +I + Q Y + RE R T +ST R+ W+SL ++ ++V + Q+ +K+
Sbjct: 141 VLEISTALSEIDRVQNYFKTREHRNYSTVKSTQARIFWFSLAESIMVVALSALQVFIVKT 200
Query: 145 FFE 137
FF+
Sbjct: 201 FFK 203
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 30.3 bits (65), Expect = 0.25
Identities = 12/40 (30%), Positives = 23/40 (57%)
Frame = -3
Query: 312 WIKYTRSQRSRLTRGKEKSVFDKHQKAQTSECSGGVCYRQ 193
W+ + S+R L + ++++V K ++ Q CS VC R+
Sbjct: 266 WLSLSESERRSLVKVEKEAVLQKMKEQQKYSCSCSVCGRK 305
>SPAC24H6.03 |cul3|pcu3|cullin 3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 785
Score = 26.2 bits (55), Expect = 4.1
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = -1
Query: 239 RKHKPASALVEFATDRCPCHHRLLADETSQEL 144
RKH+ + +V DR C H L E +++L
Sbjct: 716 RKHQADACIVRVMKDRKVCEHNQLMAEVTRQL 747
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3
Query: 480 VLDVNEIIPSNE*TRDNSILSFGSRTS 560
V D+ EI+PS T NS+ GS T+
Sbjct: 533 VTDIKEILPSQHDTPHNSVKLTGSSTT 559
>SPAC20G8.08c |fft1||fun thirty related protein
Fft1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/19 (52%), Positives = 12/19 (63%)
Frame = -2
Query: 649 YHSYSKYKVELYDPKSGGF 593
+H+ YKV L KSGGF
Sbjct: 826 FHTNENYKVFLLSTKSGGF 844
>SPBC16H5.08c |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 618
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/40 (32%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -2
Query: 385 HAVDYANVAQKEKLTELQLRIRQL--LDQVHQITKEQAYQ 272
+AVDY + K+K+ +L+ I +L D V + E Y+
Sbjct: 147 NAVDYIIQSAKDKVQKLEAEIEELSTADDVDDVLLESKYE 186
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -2
Query: 541 DRILLSRVYSLEGMISFTSS 482
DR+ LS +Y+L+ ++ F SS
Sbjct: 170 DRVFLSEIYALKILVEFLSS 189
>SPAC1687.03c |rfc4||DNA replication factor C complex subunit
Rfc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 342
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/71 (22%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = -2
Query: 478 PGEHVICMYSNSTSWFSGSQLRVHLDIQVGEHAVDYANVA-QKEKLTELQLRIRQLLDQV 302
PG H I + + S +G+Q + +++ + +A Q K+ E ++ R + +
Sbjct: 117 PGRHKIIILDEADSMTAGAQQALRRTMEIYSNTTRFALACNQSNKIIE-PIQSRCAILRY 175
Query: 301 HQITKEQAYQR 269
++T +Q QR
Sbjct: 176 SRLTDQQVLQR 186
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = -2
Query: 307 QVHQITKEQAYQRQREERFRQTSESTNQRVLWWSL 203
++ + K++ Q REER+RQ +E + ++ L
Sbjct: 535 EISKRRKQRKAQEMREERYRQRAERDTEEQIYSEL 569
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,434,494
Number of Sequences: 5004
Number of extensions: 45597
Number of successful extensions: 155
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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