Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmmt12d01
         (559 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi...    29   0.61 
SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces ...    28   0.81 
SPBC428.01c |nup107|SPBC582.11c|nucleoporin Nup107|Schizosacchar...    27   1.9  
SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces pom...    27   1.9  
SPBC530.14c |dsk1||SR protein-specific kinase Dsk1|Schizosacchar...    27   2.5  
SPBC354.05c |sre2||membrane-tethered transcription factor |Schiz...    26   3.3  
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    26   3.3  
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    26   3.3  
SPAC6B12.12 |tom70||mitochondrial TOM complex subunit Tom70|Schi...    26   3.3  
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac...    25   5.7  
SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual      25   5.7  
SPAC4F10.09c |||ribosome biogenesis protein Noc1 |Schizosaccharo...    25   5.7  
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    25   5.7  
SPBC651.07 |csa1||sequence orphan|Schizosaccharomyces pombe|chr ...    25   5.7  
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    25   7.5  
SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces...    25   7.5  
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy...    25   10.0 
SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces pombe...    25   10.0 
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    25   10.0 

>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
           Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 190

 Score = 28.7 bits (61), Expect = 0.61
 Identities = 13/47 (27%), Positives = 28/47 (59%)
 Frame = -2

Query: 372 GQVSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEF 232
           G ++K E+  ++++F   P    + + +++F + DA  +G ID +EF
Sbjct: 41  GHLNKSEFQKIYKQFF--PFGDPSAFAEYVFNVFDADKNGYIDFKEF 85



 Score = 27.5 bits (58), Expect = 1.4
 Identities = 15/66 (22%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
 Frame = -2

Query: 261 NDGSIDSEEFSSVYASFGLDKAEAASAFQKLSKGKSS--VSFAEFQELFKEYFASEDVNA 88
           +   +  ++   +  S   DK E    ++   K   S  ++ +EFQ+++K++F   D +A
Sbjct: 4   SQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSA 63

Query: 87  PGNFVF 70
              +VF
Sbjct: 64  FAEYVF 69



 Score = 26.2 bits (55), Expect = 3.3
 Identities = 31/112 (27%), Positives = 48/112 (42%), Gaps = 9/112 (8%)
 Frame = -2

Query: 540 FDTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTDNDGQVS 361
           FD + +G ID K+F  A+     SRG         E+ D L  +W      D DN+G +S
Sbjct: 72  FDADKNGYIDFKEFICALS--VTSRG---------ELNDKL--IW-AFQLYDLDNNGLIS 117

Query: 360 KEEWI----SLWEKFSS-----SPSDWQNLYCKFIFQLEDASNDGSIDSEEF 232
            +E +    ++++   S        D        IF + D + DG +  EEF
Sbjct: 118 YDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLEEF 169


>SPAC2E12.03c |||G-protein coupled receptor |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 283

 Score = 28.3 bits (60), Expect = 0.81
 Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
 Frame = -2

Query: 384 TDNDGQVSKEEWISLWE---KFSSSPSDWQNLY 295
           T+ DG    EE+  ++    KF++ P+ WQN Y
Sbjct: 240 TEEDGDEKDEEYSDIFSIKGKFNTRPNAWQNAY 272


>SPBC428.01c |nup107|SPBC582.11c|nucleoporin
           Nup107|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 794

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 16/44 (36%), Positives = 22/44 (50%)
 Frame = -2

Query: 357 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSS 226
           EEWI LW+  S +   W  +   + F+L +    G I S  FSS
Sbjct: 100 EEWIELWDLESRT---WDLIQRLYSFRLSE--QQGHIQSHAFSS 138


>SPAC2E1P5.03 |||DNAJ domain protein Erj5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 303

 Score = 27.1 bits (57), Expect = 1.9
 Identities = 16/29 (55%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = -2

Query: 396 SSADTDNDGQVSKEEWISLWEKFS-SSPS 313
           SS DTD+DGQ+  EE      K S SSPS
Sbjct: 245 SSEDTDSDGQMEDEEKSDSVHKSSFSSPS 273


>SPBC530.14c |dsk1||SR protein-specific kinase
           Dsk1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 544

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = -3

Query: 377 TTAKSPKKSGFPSGRSFPAAPVTGRI 300
           T AK+P K+  P+G+  P++P T  +
Sbjct: 278 TFAKNPTKNSKPAGQVIPSSPFTSTL 303


>SPBC354.05c |sre2||membrane-tethered transcription factor
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 793

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 20/58 (34%), Positives = 24/58 (41%), Gaps = 3/58 (5%)
 Frame = -3

Query: 383 PITTAKSPKKSGFPSGRSFPAA---PVTGRIYTASLYFNLRMLVMTGPSTARNSLRST 219
           PI+   S    G P   S  +A    V      AS  FNL   V   P  ARNS+ S+
Sbjct: 57  PISHVPSVGTRGIPRSYSMASAYPYQVNSNSMDASTAFNLNQDVADKPMLARNSIDSS 114


>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 31/108 (28%), Positives = 44/108 (40%)
 Frame = -2

Query: 537 DTNGSGTIDKKDFELAIERISKSRGWSAGDAQYKEVQDTLLKVWDGLSSADTDNDGQVSK 358
           D +G+GTID  +F   + R  K       D   +E      KV+D       D +G ++ 
Sbjct: 58  DADGNGTIDFTEFLTMMARKMK-------DTDNEEEVREAFKVFD------KDGNGYITV 104

Query: 357 EEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSSVYAS 214
           EE   +        S  Q      I +  D   DG I+ EEFS V +S
Sbjct: 105 EELTHVLTSLGERLS--QEEVADMIREA-DTDGDGVINYEEFSRVISS 149


>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 821

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 9/27 (33%), Positives = 14/27 (51%)
 Frame = -2

Query: 381 DNDGQVSKEEWISLWEKFSSSPSDWQN 301
           DND Q  +  W S+   F+  P ++ N
Sbjct: 253 DNDSQTRRSSWSSIASAFNDFPEEFPN 279


>SPAC6B12.12 |tom70||mitochondrial TOM complex subunit
           Tom70|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 26.2 bits (55), Expect = 3.3
 Identities = 15/48 (31%), Positives = 25/48 (52%)
 Frame = -2

Query: 198 AEAASAFQKLSKGKSSVSFAEFQELFKEYFASEDVNAPGNFVFGKTSF 55
           A  A A++ L KG+  +S+ + +E     F+S  VNA  + + G   F
Sbjct: 311 AALAEAYEYLEKGEYQLSYDKAKESCLGSFSSPSVNARTHNLVGTFKF 358


>SPAC18G6.05c |||translation elongation regulator Gcn1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2670

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 14/45 (31%), Positives = 24/45 (53%)
 Frame = +2

Query: 290 LQYKFCQSLGLLENFSQREIHSSLETWPSLSVSALLNPSQTFKSV 424
           L ++FC SLGLL +  Q +++      PS+  + L +P   F  +
Sbjct: 257 LYHEFCYSLGLLLSVDQLKLY----LLPSIEKALLRSPEIIFSGI 297


>SPAP27G11.11c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 73

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
 Frame = +2

Query: 302 FCQSLGLLENFSQREIHSS--LETWPSLSVSALLNPSQTF 415
           +C S+  LE  S R   SS  L    SLS+    NPSQTF
Sbjct: 26  YCVSIYRLEECSGRFKPSSYSLHLLKSLSIIICENPSQTF 65


>SPAC4F10.09c |||ribosome biogenesis protein Noc1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 860

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 16/61 (26%), Positives = 20/61 (32%)
 Frame = -2

Query: 279 QLEDASNDGSIDSEEFSSVYASFGLDKAEAASAFQKLSKGKSSVSFAEFQELFKEYFASE 100
           QLE    +   DSEE        G D  ++A      S           +E   E   SE
Sbjct: 736 QLEMDEEESDFDSEEMDKAMTDMGSDSEQSADENDNESMASEEKPMFSDEENLSEIAHSE 795

Query: 99  D 97
           D
Sbjct: 796 D 796


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 19/58 (32%), Positives = 24/58 (41%)
 Frame = +1

Query: 364 DLAVVIGVSAAQPIPDLQKRVLNFFVLSISSTPASRFGYPFNGKFKVLFINGSAAIGV 537
           D  V IG+ A +P P  +    NF+  +  S    R   PF GK    F      IGV
Sbjct: 179 DTLVSIGL-ATKPYPPFRLPGWNFWSTAYVSDGTRRSNSPFTGKPYSSFYQQGDVIGV 235


>SPBC651.07 |csa1||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 14/48 (29%), Positives = 25/48 (52%)
 Frame = -2

Query: 387 DTDNDGQVSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSID 244
           D +N  Q  KEEW +   + + +PS + +     + QL+D S   +I+
Sbjct: 137 DFNNKDQFWKEEWSTEQYEIAENPSGYASA-LHTLTQLDDGSETNAIN 183


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 21/66 (31%), Positives = 31/66 (46%)
 Frame = -2

Query: 366  VSKEEWISLWEKFSSSPSDWQNLYCKFIFQLEDASNDGSIDSEEFSSVYASFGLDKAEAA 187
            +S EE +S   +  SS  D +N   +    L+D S   S   E+  S+ +S      E A
Sbjct: 1421 ISLEESLSNQRQKESSLLDAKN---ELEHMLDDTSRKNSSLMEKIESINSSLDDKSFELA 1477

Query: 186  SAFQKL 169
            SA +KL
Sbjct: 1478 SAVEKL 1483


>SPBC17A3.05c |||DNAJ/DUF1977 DNAJB12 homolog|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 403

 Score = 25.0 bits (52), Expect = 7.5
 Identities = 8/22 (36%), Positives = 13/22 (59%)
 Frame = -2

Query: 132 QELFKEYFASEDVNAPGNFVFG 67
           ++LF  +F  +  + PG F FG
Sbjct: 212 EDLFNSFFGDQFFSGPGTFFFG 233


>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 754

 Score = 24.6 bits (51), Expect = 10.0
 Identities = 11/31 (35%), Positives = 20/31 (64%)
 Frame = -2

Query: 177 QKLSKGKSSVSFAEFQELFKEYFASEDVNAP 85
           +++ KGK+S S  E  ++FKEY + ++   P
Sbjct: 682 ERVMKGKTSPS--EMFKMFKEYLSFDEAGLP 710


>SPAC19E9.01c |nup40||nucleoporin Nup40|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 371

 Score = 24.6 bits (51), Expect = 10.0
 Identities = 11/19 (57%), Positives = 14/19 (73%)
 Frame = +1

Query: 436 FVLSISSTPASRFGYPFNG 492
           F LS+SS P+S+FG P  G
Sbjct: 82  FRLSLSSPPSSKFGGPSFG 100


>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 24.6 bits (51), Expect = 10.0
 Identities = 14/34 (41%), Positives = 23/34 (67%), Gaps = 1/34 (2%)
 Frame = -2

Query: 489 IERISKSRGWSAGDAQYKE-VQDTLLKVWDGLSS 391
           ++ I+K+RG SAGD+   + V + LL   DG++S
Sbjct: 599 LDSIAKARGASAGDSGGGDRVVNQLLTEMDGVNS 632


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,460,188
Number of Sequences: 5004
Number of extensions: 53955
Number of successful extensions: 212
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 198
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 212
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)