BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= rbmmt11m02
(683 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1 |Schizosacc... 30 0.36
SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1 |Schi... 29 0.63
SPCC1919.03c |||AMP-activated protein kinase beta subunit |Schiz... 27 3.3
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 27 3.3
SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase Met... 27 3.3
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 4.4
>SPAC17H9.10c |ddb1||damaged DNA binding protein Ddb1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1072
Score = 29.9 bits (64), Expect = 0.36
Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 2/43 (4%)
Frame = -3
Query: 600 FPRLACSNKLFSFIILV*IHS--GTVHVQLLQVQEAIKKRALS 478
FPR CSN LF F++ + + GT +LL +K LS
Sbjct: 703 FPRRICSNSLFYFVLCMQLEESIGTQEQRLLSFLRVYEKNTLS 745
>SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 454
Score = 29.1 bits (62), Expect = 0.63
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
Frame = +1
Query: 61 HRFAWR-VSQFVTEGWVSRNNLF--SLVTVLGASYQLRYVQFGDGEQIHQQVLLVRVERA 231
H F W+ + Q++ G V N+ L +RY+ F + E++ ++ L + R
Sbjct: 41 HLFPWKYLFQWLNHGPVVTNDFAHREFAFTLPNDAYIRYLSFSNWEELKKEALNLCPSRF 100
Query: 232 LVGPVHFA 255
VGPV+ A
Sbjct: 101 EVGPVYSA 108
>SPCC1919.03c |||AMP-activated protein kinase beta subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 298
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -3
Query: 282 PKMKDWRLPREVDWADQRALYTNQKHLLMNLLAIPKLDIPELITSAEDCYEREKVISA 109
P ++ L + + +++ N H+L+N LA + L SA Y R+ V +A
Sbjct: 234 PHLEKCILNSNTAYKEDQSVLPNPNHVLLNHLAAANTQLGVLALSATTRYHRKYVTTA 291
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 216 NQKHLLMNLLAIPKLDIPELITSAE 142
NQ+H L++ IP LD + +TS E
Sbjct: 1306 NQRHTLISAGRIPNLDFSDSVTSME 1330
>SPAC343.10 |met11|mthfr2|methylenetetrahydrofolate reductase
Met11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.6 bits (56), Expect = 3.3
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = -3
Query: 276 MKDWRLPREVDWADQRALYTNQKHLLMNLLAIPKLDIP 163
+K W P VD +D +L+ QKH++ ++ AIP +D P
Sbjct: 429 LKLWGYP--VDESDITSLF--QKHIMSDISAIPWIDEP 462
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 26.2 bits (55), Expect = 4.4
Identities = 15/59 (25%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = -3
Query: 531 VHVQLLQVQEAIKKR--ALSHEVTSKALTPAFLTEIEKQYKNKYLRDIATHAELLVYDW 361
+ LL + I+++ + +V S L P FL E E Y+ + L + ++ +Y W
Sbjct: 1913 LETNLLPASDTIEEKNDVGNKQVNSHILDPGFLKEEECVYEFEQLHGLRKALQVEIYGW 1971
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,657,539
Number of Sequences: 5004
Number of extensions: 51298
Number of successful extensions: 149
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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