Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= rbmmt10c03
         (689 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   238   6e-64
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   238   6e-64
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   238   6e-64
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    80   3e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    49   6e-07
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    26   4.5  
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    26   5.9  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   7.8  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  238 bits (582), Expect = 6e-64
 Identities = 107/139 (76%), Positives = 123/139 (88%)
 Frame = -3

Query: 678 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 499
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 498 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 319
           CHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420

Query: 318 RFAVRDMRQTVAVGVIKAV 262
           RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  238 bits (582), Expect = 6e-64
 Identities = 107/139 (76%), Positives = 123/139 (88%)
 Frame = -3

Query: 678 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 499
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 498 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 319
           CHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420

Query: 318 RFAVRDMRQTVAVGVIKAV 262
           RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  238 bits (582), Expect = 6e-64
 Identities = 107/139 (76%), Positives = 123/139 (88%)
 Frame = -3

Query: 678 VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 499
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 498 CHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLG 319
           CHTAHIACKFAE+ EK+DRR+GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLG
Sbjct: 361 CHTAHIACKFAELIEKIDRRSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLG 420

Query: 318 RFAVRDMRQTVAVGVIKAV 262
           RFAVRDMRQTVAVGVIKAV
Sbjct: 421 RFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 79.8 bits (188), Expect = 3e-16
 Identities = 46/142 (32%), Positives = 76/142 (53%), Gaps = 1/142 (0%)
 Frame = -3

Query: 681 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 502
           ++ GD V   V+     +++ GYV   +KN P      F AQ+ +L  P  ++ GY+ V+
Sbjct: 524 SICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVM 581

Query: 501 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 322
             HTA     FA++  K+D+ T + ++  P     G   I  L    P+C+E F+++  +
Sbjct: 582 HIHTAVEEVSFAKLLHKLDK-TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYM 640

Query: 321 GRFAVRDMRQTVAVG-VIKAVN 259
           GRF +RD   TVAVG V+K ++
Sbjct: 641 GRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 41/138 (29%), Positives = 63/138 (45%)
 Frame = -3

Query: 681 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 502
           AV GD V   + ++ V +LR G +  + +N P +    F A++   +  G I +G T VL
Sbjct: 461 AVAGDTVTLQLADIEVNQLRPGDILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL 519

Query: 501 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 322
                H+      +  K+     K +  +  S K     I  L    PLC+   +E P L
Sbjct: 520 -----HLGRTVTSVSLKIVTVNNKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPAL 571

Query: 321 GRFAVRDMRQTVAVGVIK 268
           GRF +R    TVA G++K
Sbjct: 572 GRFILRRSGDTVAAGIVK 589


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 28/137 (20%), Positives = 55/137 (40%)
 Frame = -3

Query: 681 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 502
           AV GDN G  ++++  ++L+RG +        P     F A   +L    +     T  +
Sbjct: 311 AVAGDNCGLLLRSIKREQLKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFV 366

Query: 501 DCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPL 322
           D +   +  + +++  ++   T      + K +  GD   +      P+ +E  Q     
Sbjct: 367 DKYRPQLYSRTSDVTVEL---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ----- 418

Query: 321 GRFAVRDMRQTVAVGVI 271
            RF VR+   TV   ++
Sbjct: 419 -RFTVREGGSTVGTALV 434


>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
 Frame = +2

Query: 218 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 391
           S  +VTLPPPAS   ++  T T T  + S ++     G+ + +++        +  ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241

Query: 392 SPDLMDFGLTSVDLPVRRSTFSLIS 466
           S  L    +TS   PV  S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = +2

Query: 383 MAASPDLMDFGLTSVDLPVRRSTFS 457
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,800,684
Number of Sequences: 5004
Number of extensions: 56423
Number of successful extensions: 159
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)