BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV31053.Seq (668 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 116 2e-27 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 116 2e-27 SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces ... 33 0.049 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.4 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 28 1.4 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 26 5.6 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 5.6 SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 9.9 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 25 9.9 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 116 bits (280), Expect = 2e-27 Identities = 53/75 (70%), Positives = 62/75 (82%) Frame = +1 Query: 31 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 210 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 211 DTGNFSWGSECSTRK 255 D+GNFSWGSE ++K Sbjct: 61 DSGNFSWGSEGVSKK 75 Score = 77.8 bits (183), Expect = 1e-15 Identities = 42/87 (48%), Positives = 55/87 (63%) Frame = +3 Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEA 425 + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G KG K T Sbjct: 73 SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATST 131 Query: 426 EEAIINKKRSQKTARKYLARQRLAKVE 506 +S+ RK+ AR +KV+ Sbjct: 132 P-----NPKSKHVQRKHSARLGDSKVD 153 Score = 67.7 bits (158), Expect = 1e-12 Identities = 31/46 (67%), Positives = 36/46 (78%) Frame = +2 Query: 509 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 646 ALE QF GRL A V+SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 116 bits (280), Expect = 2e-27 Identities = 53/75 (70%), Positives = 62/75 (82%) Frame = +1 Query: 31 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 210 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 211 DTGNFSWGSECSTRK 255 D+GNFSWGSE ++K Sbjct: 61 DSGNFSWGSEGVSKK 75 Score = 77.0 bits (181), Expect = 2e-15 Identities = 42/87 (48%), Positives = 55/87 (63%) Frame = +3 Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEA 425 + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G KG K T Sbjct: 73 SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATAT 131 Query: 426 EEAIINKKRSQKTARKYLARQRLAKVE 506 +S+ RK+ AR +KV+ Sbjct: 132 P-----TPKSKHVQRKHSARLGDSKVD 153 Score = 67.7 bits (158), Expect = 1e-12 Identities = 31/46 (67%), Positives = 36/46 (78%) Frame = +2 Query: 509 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKELEFYLRKIKSKR 646 ALE QF GRL A V+SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces pombe|chr 1|||Manual Length = 655 Score = 32.7 bits (71), Expect = 0.049 Identities = 16/40 (40%), Positives = 21/40 (52%) Frame = -1 Query: 623 DRTRVLCLLRCNHRRDHTDLGDSPRKPANALCGIALLEPL 504 D + CLLR H+RD + + RK N L G LLE + Sbjct: 434 DANIIPCLLRSKHKRDKANPENKERKLVNNLLGAQLLEEM 473 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.9 bits (59), Expect = 1.4 Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 2/74 (2%) Frame = +1 Query: 43 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 216 RD +K + G K+ + EL +N + Q+ + S T YRA+ LDT Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142 Query: 217 GNFSWGSECSTRKP 258 G+ E + KP Sbjct: 1143 GDLMAVKEVALHKP 1156 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 27.9 bits (59), Expect = 1.4 Identities = 17/54 (31%), Positives = 26/54 (48%) Frame = +1 Query: 46 DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 207 + W K T K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.8 bits (54), Expect = 5.6 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = +1 Query: 19 DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 135 + TK G + RRA A RKK K + GRP +N Sbjct: 3 ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 5.6 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -2 Query: 367 YHCLNGVASTTTIAFLTRVFVRTNS 293 ++C N V S T + + VFVR NS Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542 >SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 689 Score = 25.0 bits (52), Expect = 9.9 Identities = 12/60 (20%), Positives = 29/60 (48%), Gaps = 7/60 (11%) Frame = -1 Query: 338 YNNCILDKGLCTHQFIVRCIIH-------NINDTGLRVEHSDPQEKLPVSRRSARYLVFP 180 + N + + +H FI R +I+ +I D G ++ + ++ L + + Y+++P Sbjct: 540 FENSLYHQSFSSHWFISRLLINPIRMLCWHITDVGKTLDLGEAEKLLKYNHKQCPYIIYP 599 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 25.0 bits (52), Expect = 9.9 Identities = 8/23 (34%), Positives = 14/23 (60%) Frame = +1 Query: 46 DHWHKRRATGGKRAPIRKKRKYE 114 D W +A G +PIR+ ++Y+ Sbjct: 758 DEWKASKAFGANDSPIRENKRYK 780 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,654,585 Number of Sequences: 5004 Number of extensions: 53375 Number of successful extensions: 154 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 146 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 154 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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