BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV31029.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 100 1e-22 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 100 1e-22 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 30 0.29 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 27 2.7 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 25 6.3 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 100 bits (240), Expect = 1e-22 Identities = 46/65 (70%), Positives = 54/65 (83%) Frame = +2 Query: 62 KRAPIRKKRKYELXRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKT 241 KRA RKKRK+EL R +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++KT Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKKT 76 Query: 242 RIIDV 256 RII V Sbjct: 77 RIIQV 81 Score = 74.1 bits (174), Expect = 1e-14 Identities = 46/102 (45%), Positives = 57/102 (55%) Frame = +1 Query: 259 YNASNNXLVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKXGAKLXXAXEAIXNKKRS 438 Y+ SNN LVRT TL K+AIV +DA PFR WYE+HY + +G K G K + N K S Sbjct: 83 YHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMGSK-GKK----ATSTPNPK-S 136 Query: 439 XKTARKYLARQRLAKVEGALEXQSTQGVCWLAWRVAXGQCGR 564 RK+ AR +KV+ ALE Q G + GQ GR Sbjct: 137 KHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPGQSGR 178 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 100 bits (240), Expect = 1e-22 Identities = 46/65 (70%), Positives = 54/65 (83%) Frame = +2 Query: 62 KRAPIRKKRKYELXRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKT 241 KRA RKKRK+EL R +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++KT Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKKT 76 Query: 242 RIIDV 256 RII V Sbjct: 77 RIIQV 81 Score = 73.3 bits (172), Expect = 2e-14 Identities = 44/102 (43%), Positives = 55/102 (53%) Frame = +1 Query: 259 YNASNNXLVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKXGAKLXXAXEAIXNKKRS 438 Y+ SNN LVRT TL K+AIV +DA PFR WYE+HY + +G K G K +S Sbjct: 83 YHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMGSK-GKKATATP-----TPKS 136 Query: 439 XKTARKYLARQRLAKVEGALEXQSTQGVCWLAWRVAXGQCGR 564 RK+ AR +KV+ ALE Q G + GQ GR Sbjct: 137 KHVQRKHSARLGDSKVDSALETQFAAGRLYAVVSSRPGQSGR 178 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 29.9 bits (64), Expect = 0.29 Identities = 16/50 (32%), Positives = 25/50 (50%) Frame = +2 Query: 41 SEGPPAGKRAPIRKKRKYELXRPAANTRLGPQRIHSVRSRGGNTKYRALR 190 S+G K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 35 SKGVKTNKKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 26.6 bits (56), Expect = 2.7 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = +2 Query: 95 ELXRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKTRI 247 EL +N + Q+ + S T YRA+ LDTG+ E + K RI Sbjct: 1108 ELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDTGDLMAVKEVALHKPRI 1158 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = -3 Query: 347 HCLNGVASTTTIAFLTRVFVRTNXLLDALYTHQ 249 H + ST L R+FV+ N +L LY H+ Sbjct: 318 HGAKYMTSTEVRNHLRRLFVKENVVLSRLYAHK 350 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,326,008 Number of Sequences: 5004 Number of extensions: 43340 Number of successful extensions: 101 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 93 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 99 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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