BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV31016.Seq
(489 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyc... 115 4e-27
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-... 25 4.6
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 25 6.1
SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ... 25 6.1
SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces po... 25 6.1
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 25 8.1
>SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 126
Score = 115 bits (276), Expect = 4e-27
Identities = 50/83 (60%), Positives = 70/83 (84%)
Frame = +3
Query: 6 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 185
MKF++ VTSSRRK RK HF APS +RRVLMS+PLSKELR+++ ++S+P+R+DD++ V+RG
Sbjct: 1 MKFSRDVTSSRRKQRKAHFGAPSSVRRVLMSAPLSKELREQYKIRSLPVRRDDQITVIRG 60
Query: 186 HYKGQQVGKVMQVYRKKFVVYIE 254
KG++ GK+ VYRKKF++ IE
Sbjct: 61 SNKGRE-GKITSVYRKKFLLLIE 82
Score = 46.4 bits (105), Expect = 2e-06
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = +2
Query: 257 IQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRR 370
+ REKANGA+A VGI SK VI KL ++KDRK ++ R+
Sbjct: 84 VTREKANGASAPVGIDASKVVITKLHLDKDRKDLIVRK 121
>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
[UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 25.4 bits (53), Expect = 4.6
Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +2
Query: 293 VGIHPSKCV-IVKLKMNKDRKAILDRRAKGRLAALGKDKGKY 415
+GI P K +K + KDR A ++RR +G +G D+ Y
Sbjct: 256 IGIDPEKFSDALKSDVVKDRIASIERRLQGVKVIVGVDRLDY 297
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 25.0 bits (52), Expect = 6.1
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = -3
Query: 415 VFTLVFAKCSQSALCSAIEDCFAVFIH 335
+F LV S +C IED F IH
Sbjct: 588 LFNLVATNASDPYICGIIEDTFEDIIH 614
>SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1150
Score = 25.0 bits (52), Expect = 6.1
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = +1
Query: 223 CIVKSLLYTLRDSKRKGQWCNSICRHSP 306
C K LLY R R +W N I SP
Sbjct: 388 CCAKILLYARRQLTRMARWNNLIDDSSP 415
>SPBC15C4.02 |||ABC1 kinase family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 594
Score = 25.0 bits (52), Expect = 6.1
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -2
Query: 281 HHWPFLFESLNVYNKLFTIHLHHFANLLAFVVSTYNLNFIVF 156
++W F S N+ F +H+H L+ + S Y++ F+ F
Sbjct: 20 NYWHFWLRSFMSNNRKFLVHIH----LIPHLNSLYSICFLGF 57
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 24.6 bits (51), Expect = 8.1
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 175 LYVDTTKANRLAK*CRCIVKSLLYTLR 255
LY D + A+ C C+V++LLY R
Sbjct: 78 LYHDNHERKMSAQSCACMVRTLLYGKR 104
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,021,985
Number of Sequences: 5004
Number of extensions: 42946
Number of successful extensions: 147
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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