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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV31016.Seq
         (489 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)           133   7e-32
At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S ...   128   3e-30
At3g04690.1 68416.m00503 protein kinase family protein contains ...    30   0.96 
At1g55130.1 68414.m06296 endomembrane protein 70, putative simil...    28   2.9  
At1g28070.1 68414.m03436 expressed protein ; expression supporte...    28   2.9  
At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ...    28   3.9  
At5g23535.1 68418.m02762 KOW domain-containing protein simlar to...    27   5.1  
At4g35380.1 68417.m05026 guanine nucleotide exchange family prot...    27   5.1  
At4g00900.1 68417.m00122 calcium-transporting ATPase 2, endoplas...    27   6.8  
At4g02110.1 68417.m00282 BRCT domain-containing protein contains...    27   9.0  

>At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B)
          Length = 146

 Score =  133 bits (321), Expect = 7e-32
 Identities = 63/83 (75%), Positives = 76/83 (91%)
 Frame = +3

Query: 6   MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 185
           MKFN +V+SSRRKNRK HF+APS +RRVLMSSPLSK+LR K NV+SMPIRKDDEVQVVRG
Sbjct: 1   MKFNPRVSSSRRKNRKAHFTAPSSVRRVLMSSPLSKDLRNKHNVRSMPIRKDDEVQVVRG 60

Query: 186 HYKGQQVGKVMQVYRKKFVVYIE 254
            +KG++ GKVMQVYR+K+V++IE
Sbjct: 61  TFKGRE-GKVMQVYRRKWVIHIE 82



 Score = 58.0 bits (134), Expect = 3e-09
 Identities = 27/51 (52%), Positives = 39/51 (76%)
 Frame = +2

Query: 257 IQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 409
           I REK NG+T  VG++ S  +I KL+++KDRK++L+R+A GR AA  K+KG
Sbjct: 84  ITREKVNGSTVNVGVNASNVMITKLRLDKDRKSLLERKANGR-AAADKEKG 133


>At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S
           RIBOSOMAL PROTEIN L26, Brassica rapa, EMBL:BRD495
          Length = 146

 Score =  128 bits (308), Expect = 3e-30
 Identities = 60/83 (72%), Positives = 75/83 (90%)
 Frame = +3

Query: 6   MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 185
           MK+N +VTSSRRKNRK HF+A S  RRV+MSSPLS +LRQK+NV+SMPIRKDDEVQ+VRG
Sbjct: 1   MKYNPRVTSSRRKNRKAHFTASSSERRVIMSSPLSTDLRQKYNVRSMPIRKDDEVQIVRG 60

Query: 186 HYKGQQVGKVMQVYRKKFVVYIE 254
            YKG++ GKV+QVYR+K+V++IE
Sbjct: 61  TYKGRE-GKVVQVYRRKWVIHIE 82



 Score = 66.1 bits (154), Expect = 1e-11
 Identities = 32/51 (62%), Positives = 40/51 (78%)
 Frame = +2

Query: 257 IQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 409
           I REK NG T  VGI PSK VI KL+++KDRK++L+R+AKGR AA  K+KG
Sbjct: 84  ITREKVNGTTVNVGIQPSKVVITKLRLDKDRKSLLERKAKGR-AAADKEKG 133


>At3g04690.1 68416.m00503 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 850

 Score = 29.9 bits (64), Expect = 0.96
 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = -2

Query: 311 LKGECRHML-LHHWPFLFESLNVYNKLFTIHLHHFANLLAF 192
           +KG+ RH+L L+ +P  +  LN+ N  FT+  +    L  F
Sbjct: 93  IKGDKRHLLRLYFYPSTYTGLNISNSYFTVEANDVTLLSNF 133


>At1g55130.1 68414.m06296 endomembrane protein 70, putative similar
           to multispanning membrane protein GI:2276460 from [Homo
           sapiens]
          Length = 637

 Score = 28.3 bits (60), Expect = 2.9
 Identities = 21/73 (28%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
 Frame = +3

Query: 84  RVLMSSPLSKELRQK--FNVKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKKFVVYIEG 257
           RV + +  +K  R+K  +  ++  I  +  V V+R    G Q       YR  F    EG
Sbjct: 105 RVRVDAESAKNFREKIDYEYRANMILDNLPVAVLRQRKDGIQSTTYEHGYRVGFKGSYEG 164

Query: 258 FKEKRPMVQQHMS 296
            KEK+  +  H+S
Sbjct: 165 SKEKKYFIHNHLS 177


>At1g28070.1 68414.m03436 expressed protein ; expression supported
           by MPSS
          Length = 176

 Score = 28.3 bits (60), Expect = 2.9
 Identities = 17/61 (27%), Positives = 28/61 (45%)
 Frame = +3

Query: 30  SSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVG 209
           S R      HFS PSH+ + L     + E+  +     M +++D    + RG+Y  Q  G
Sbjct: 38  SDRFLELSSHFSVPSHLEQCL--DLKTGEIYYRSWNSGMRVKEDPRKSMSRGNYADQSSG 95

Query: 210 K 212
           +
Sbjct: 96  E 96


>At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains
           similarity to NAD kinase [Homo sapiens]
           gi|20070086|gb|AAM01195; contains Pfam domain, PF01513:
           ATP-NAD kinase
          Length = 985

 Score = 27.9 bits (59), Expect = 3.9
 Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 3/80 (3%)
 Frame = +3

Query: 75  HIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKK---FVV 245
           ++ R + S  + +   Q+ N  S     DDE   + G+      G V    RKK   F+V
Sbjct: 583 NLSRAVGSHSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCASATGVVRVQSRKKAEMFLV 642

Query: 246 YIEGFKEKRPMVQQHMSAFT 305
             +G    R  V +   AFT
Sbjct: 643 RTDGVSCTREKVTESSLAFT 662


>At5g23535.1 68418.m02762 KOW domain-containing protein simlar to
           SP|P12876 50S ribosomal protein L24 (BL23) (12 kDa
           DNA-binding protein) (HPB12) {Bacillus subtilis};
           contains Pfam profile PF00467: KOW motif
          Length = 159

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 13/41 (31%), Positives = 23/41 (56%)
 Frame = +3

Query: 135 VKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKKFVVYIEG 257
           ++   I + D V ++RG  KG + G + +V R +  V +EG
Sbjct: 10  IRHWKILRGDNVMIIRGKDKG-ETGTIKRVIRSQNRVIVEG 49


>At4g35380.1 68417.m05026 guanine nucleotide exchange family protein
            similar to guanine nucleotide exchange factor [Homo
            sapiens] GI:5456754; contains Pfam profile PF01369: Sec7
            domain
          Length = 1706

 Score = 27.5 bits (58), Expect = 5.1
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +1

Query: 31   PQEGKTGRGISVLLHI 78
            P +G TG GISVLLH+
Sbjct: 1408 PFQGSTGSGISVLLHL 1423


>At4g00900.1 68417.m00122 calcium-transporting ATPase 2, endoplasmic
           reticulum-type (ECA2) nearly identical to SP|O23087
           Calcium-transporting ATPase 2, endoplasmic
           reticulum-type (EC 3.6.3.8) {Arabidopsis thaliana};
           contains InterPro Accession IPR006069: Cation
           transporting ATPase
          Length = 1054

 Score = 27.1 bits (57), Expect = 6.8
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = -3

Query: 367 AIEDCFAVFIHLQLDNHTL*RVNADICCCTIGLFSLNPSMYTTNFLRYTCITLP 206
           AIEDC    I + +      +  A+  CC I LFS N  +  ++F     ++LP
Sbjct: 632 AIEDCRDAGIRVMVITGDN-KSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLP 684


>At4g02110.1 68417.m00282 BRCT domain-containing protein contains
           Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain
          Length = 1293

 Score = 26.6 bits (56), Expect = 9.0
 Identities = 13/53 (24%), Positives = 29/53 (54%)
 Frame = +3

Query: 9   KFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDE 167
           K     ++S  ++ +RH S  ++ R+ L  SP +  L ++ + ++  +R DD+
Sbjct: 269 KMETDGSTSINRSIRRHSSLATYSRKTLQRSPETDTLGKESSGQNRSLRMDDK 321


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,614,735
Number of Sequences: 28952
Number of extensions: 229474
Number of successful extensions: 643
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 641
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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