BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV31016.Seq (489 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B) 133 7e-32 At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S ... 128 3e-30 At3g04690.1 68416.m00503 protein kinase family protein contains ... 30 0.96 At1g55130.1 68414.m06296 endomembrane protein 70, putative simil... 28 2.9 At1g28070.1 68414.m03436 expressed protein ; expression supporte... 28 2.9 At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains ... 28 3.9 At5g23535.1 68418.m02762 KOW domain-containing protein simlar to... 27 5.1 At4g35380.1 68417.m05026 guanine nucleotide exchange family prot... 27 5.1 At4g00900.1 68417.m00122 calcium-transporting ATPase 2, endoplas... 27 6.8 At4g02110.1 68417.m00282 BRCT domain-containing protein contains... 27 9.0 >At5g67510.1 68418.m08513 60S ribosomal protein L26 (RPL26B) Length = 146 Score = 133 bits (321), Expect = 7e-32 Identities = 63/83 (75%), Positives = 76/83 (91%) Frame = +3 Query: 6 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 185 MKFN +V+SSRRKNRK HF+APS +RRVLMSSPLSK+LR K NV+SMPIRKDDEVQVVRG Sbjct: 1 MKFNPRVSSSRRKNRKAHFTAPSSVRRVLMSSPLSKDLRNKHNVRSMPIRKDDEVQVVRG 60 Query: 186 HYKGQQVGKVMQVYRKKFVVYIE 254 +KG++ GKVMQVYR+K+V++IE Sbjct: 61 TFKGRE-GKVMQVYRRKWVIHIE 82 Score = 58.0 bits (134), Expect = 3e-09 Identities = 27/51 (52%), Positives = 39/51 (76%) Frame = +2 Query: 257 IQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 409 I REK NG+T VG++ S +I KL+++KDRK++L+R+A GR AA K+KG Sbjct: 84 ITREKVNGSTVNVGVNASNVMITKLRLDKDRKSLLERKANGR-AAADKEKG 133 >At3g49910.1 68416.m05456 60S ribosomal protein L26 (RPL26A) 60S RIBOSOMAL PROTEIN L26, Brassica rapa, EMBL:BRD495 Length = 146 Score = 128 bits (308), Expect = 3e-30 Identities = 60/83 (72%), Positives = 75/83 (90%) Frame = +3 Query: 6 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 185 MK+N +VTSSRRKNRK HF+A S RRV+MSSPLS +LRQK+NV+SMPIRKDDEVQ+VRG Sbjct: 1 MKYNPRVTSSRRKNRKAHFTASSSERRVIMSSPLSTDLRQKYNVRSMPIRKDDEVQIVRG 60 Query: 186 HYKGQQVGKVMQVYRKKFVVYIE 254 YKG++ GKV+QVYR+K+V++IE Sbjct: 61 TYKGRE-GKVVQVYRRKWVIHIE 82 Score = 66.1 bits (154), Expect = 1e-11 Identities = 32/51 (62%), Positives = 40/51 (78%) Frame = +2 Query: 257 IQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILDRRAKGRLAALGKDKG 409 I REK NG T VGI PSK VI KL+++KDRK++L+R+AKGR AA K+KG Sbjct: 84 ITREKVNGTTVNVGIQPSKVVITKLRLDKDRKSLLERKAKGR-AAADKEKG 133 >At3g04690.1 68416.m00503 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 850 Score = 29.9 bits (64), Expect = 0.96 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = -2 Query: 311 LKGECRHML-LHHWPFLFESLNVYNKLFTIHLHHFANLLAF 192 +KG+ RH+L L+ +P + LN+ N FT+ + L F Sbjct: 93 IKGDKRHLLRLYFYPSTYTGLNISNSYFTVEANDVTLLSNF 133 >At1g55130.1 68414.m06296 endomembrane protein 70, putative similar to multispanning membrane protein GI:2276460 from [Homo sapiens] Length = 637 Score = 28.3 bits (60), Expect = 2.9 Identities = 21/73 (28%), Positives = 33/73 (45%), Gaps = 2/73 (2%) Frame = +3 Query: 84 RVLMSSPLSKELRQK--FNVKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKKFVVYIEG 257 RV + + +K R+K + ++ I + V V+R G Q YR F EG Sbjct: 105 RVRVDAESAKNFREKIDYEYRANMILDNLPVAVLRQRKDGIQSTTYEHGYRVGFKGSYEG 164 Query: 258 FKEKRPMVQQHMS 296 KEK+ + H+S Sbjct: 165 SKEKKYFIHNHLS 177 >At1g28070.1 68414.m03436 expressed protein ; expression supported by MPSS Length = 176 Score = 28.3 bits (60), Expect = 2.9 Identities = 17/61 (27%), Positives = 28/61 (45%) Frame = +3 Query: 30 SSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVG 209 S R HFS PSH+ + L + E+ + M +++D + RG+Y Q G Sbjct: 38 SDRFLELSSHFSVPSHLEQCL--DLKTGEIYYRSWNSGMRVKEDPRKSMSRGNYADQSSG 95 Query: 210 K 212 + Sbjct: 96 E 96 >At1g21640.1 68414.m02709 ATP-NAD kinase family protein contains similarity to NAD kinase [Homo sapiens] gi|20070086|gb|AAM01195; contains Pfam domain, PF01513: ATP-NAD kinase Length = 985 Score = 27.9 bits (59), Expect = 3.9 Identities = 22/80 (27%), Positives = 33/80 (41%), Gaps = 3/80 (3%) Frame = +3 Query: 75 HIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKK---FVV 245 ++ R + S + + Q+ N S DDE + G+ G V RKK F+V Sbjct: 583 NLSRAVGSHSVRESQTQRNNSGSSSDSSDDEAGAIEGNMCASATGVVRVQSRKKAEMFLV 642 Query: 246 YIEGFKEKRPMVQQHMSAFT 305 +G R V + AFT Sbjct: 643 RTDGVSCTREKVTESSLAFT 662 >At5g23535.1 68418.m02762 KOW domain-containing protein simlar to SP|P12876 50S ribosomal protein L24 (BL23) (12 kDa DNA-binding protein) (HPB12) {Bacillus subtilis}; contains Pfam profile PF00467: KOW motif Length = 159 Score = 27.5 bits (58), Expect = 5.1 Identities = 13/41 (31%), Positives = 23/41 (56%) Frame = +3 Query: 135 VKSMPIRKDDEVQVVRGHYKGQQVGKVMQVYRKKFVVYIEG 257 ++ I + D V ++RG KG + G + +V R + V +EG Sbjct: 10 IRHWKILRGDNVMIIRGKDKG-ETGTIKRVIRSQNRVIVEG 49 >At4g35380.1 68417.m05026 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor [Homo sapiens] GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1706 Score = 27.5 bits (58), Expect = 5.1 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 31 PQEGKTGRGISVLLHI 78 P +G TG GISVLLH+ Sbjct: 1408 PFQGSTGSGISVLLHL 1423 >At4g00900.1 68417.m00122 calcium-transporting ATPase 2, endoplasmic reticulum-type (ECA2) nearly identical to SP|O23087 Calcium-transporting ATPase 2, endoplasmic reticulum-type (EC 3.6.3.8) {Arabidopsis thaliana}; contains InterPro Accession IPR006069: Cation transporting ATPase Length = 1054 Score = 27.1 bits (57), Expect = 6.8 Identities = 17/54 (31%), Positives = 26/54 (48%) Frame = -3 Query: 367 AIEDCFAVFIHLQLDNHTL*RVNADICCCTIGLFSLNPSMYTTNFLRYTCITLP 206 AIEDC I + + + A+ CC I LFS N + ++F ++LP Sbjct: 632 AIEDCRDAGIRVMVITGDN-KSTAEAICCEIRLFSENEDLSQSSFTGKEFMSLP 684 >At4g02110.1 68417.m00282 BRCT domain-containing protein contains Pfam domain, PF00533: BRCA1 C Terminus (BRCT) domain Length = 1293 Score = 26.6 bits (56), Expect = 9.0 Identities = 13/53 (24%), Positives = 29/53 (54%) Frame = +3 Query: 9 KFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDE 167 K ++S ++ +RH S ++ R+ L SP + L ++ + ++ +R DD+ Sbjct: 269 KMETDGSTSINRSIRRHSSLATYSRKTLQRSPETDTLGKESSGQNRSLRMDDK 321 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,614,735 Number of Sequences: 28952 Number of extensions: 229474 Number of successful extensions: 643 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 632 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 641 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 848837888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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