BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV31014.Seq (349 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 77 8e-16 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 51 6e-08 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 1.1 SPBC17G9.02c |||RNA polymerase II accessory factor, Cdc73 family... 26 1.5 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 1.9 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 24 5.9 SPBC1861.07 |||elongin C|Schizosaccharomyces pombe|chr 2|||Manual 24 7.8 SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharo... 24 7.8 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 77.0 bits (181), Expect = 8e-16 Identities = 35/87 (40%), Positives = 55/87 (63%), Gaps = 2/87 (2%) Frame = +1 Query: 1 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 174 +++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE Sbjct: 21 LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80 Query: 175 AELNYAMDVYELNXRVNSSESIXGLWA 255 E+ Y +Y L+ + N E + G +A Sbjct: 81 VEMEYHRAMYHLHLKANPREVVVGWYA 107 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 50.8 bits (116), Expect = 6e-08 Identities = 27/84 (32%), Positives = 44/84 (52%), Gaps = 4/84 (4%) Frame = +1 Query: 7 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 177 V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76 Query: 178 -ELNYAMDVYELNXRVNSSESIXG 246 + N+ + E+ ++N++E + G Sbjct: 77 LDHNFMESMNEMFKKINANEKLVG 100 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 1.1 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 49 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 174 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPBC17G9.02c |||RNA polymerase II accessory factor, Cdc73 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 371 Score = 26.2 bits (55), Expect = 1.5 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +3 Query: 243 WFVGTXNEVTNHFSVIHEYYPVNAVSL 323 W V+NH SV+H P++ +SL Sbjct: 147 WLFENTRTVSNHNSVLHGIKPIDFISL 173 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 1.9 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 60 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 203 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 24.2 bits (50), Expect = 5.9 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +3 Query: 42 GRIRTSKCRFSQSYRHLIGHERQRS 116 G+ KCR ++ HL+GHE + S Sbjct: 281 GQYHKYKCRPAEYVCHLLGHEGEGS 305 >SPBC1861.07 |||elongin C|Schizosaccharomyces pombe|chr 2|||Manual Length = 97 Score = 23.8 bits (49), Expect = 7.8 Identities = 9/23 (39%), Positives = 15/23 (65%) Frame = -1 Query: 79 LCENLHFDVRMRPQFEIKQQDGP 11 +CE LH++ R + Q +I + D P Sbjct: 59 VCEYLHYNYRYKNQLDIPKFDIP 81 >SPBC13A2.03 |||phosphatidate cytidylyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 23.8 bits (49), Expect = 7.8 Identities = 8/29 (27%), Positives = 15/29 (51%) Frame = -2 Query: 192 RVIKFRFDLIGMFFVWHAEAVGYFHHSFV 106 R I + F + +++ + YFHH F+ Sbjct: 109 RFINWYFLMTTLYYAYGESIYAYFHHLFI 137 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,468,140 Number of Sequences: 5004 Number of extensions: 26070 Number of successful extensions: 76 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 72 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 74 length of database: 2,362,478 effective HSP length: 64 effective length of database: 2,042,222 effective search space used: 104153322 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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