BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV31003.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 88 8e-19 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 59 4e-10 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.7 SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 26 4.8 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 4.8 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 6.3 SPAC227.08c |yth1||zinc finger protein Yth1|Schizosaccharomyces ... 25 8.4 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 88.2 bits (209), Expect = 8e-19 Identities = 55/175 (31%), Positives = 88/175 (50%), Gaps = 14/175 (8%) Frame = +1 Query: 1 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 174 +++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE Sbjct: 21 LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80 Query: 175 AELNYAMDVYELNRRVNSSESIVGWWALAMK*PTTPL----LYTSIIPVNAVSL------ 324 E+ Y +Y L+ + N E +VGW+A + LY S L Sbjct: 81 VEMEYHRAMYHLHLKANPREVVVGWYATSPDLDAFSALIQNLYASPAEPGTAPLGTYPHP 140 Query: 325 -XHVTLDTSLAGGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 483 H+T++T ++ + ++ YV P+G+ C F P T+ E GL+ Sbjct: 141 CVHLTVNTDVS-SPLAIKTYVSSPVGITERLADSCAFVPTPFTIRDDEAVRSGLK 194 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 59.3 bits (137), Expect = 4e-10 Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 4/86 (4%) Frame = +1 Query: 7 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 177 V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76 Query: 178 -ELNYAMDVYELNRRVNSSESIVGWW 252 + N+ + E+ +++N++E +VGW+ Sbjct: 77 LDHNFMESMNEMFKKINANEKLVGWY 102 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.7 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 49 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 174 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|||Manual Length = 997 Score = 25.8 bits (54), Expect = 4.8 Identities = 9/24 (37%), Positives = 14/24 (58%) Frame = -3 Query: 551 RQVRARGHAARPATSPDTVFWHTC 480 +Q+ RG + + PD +FW TC Sbjct: 950 KQMNRRGATSIVSVRPDPLFWPTC 973 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 4.8 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 60 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 203 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 25.4 bits (53), Expect = 6.3 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +3 Query: 423 HVHSCRCHSHM 455 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 >SPAC227.08c |yth1||zinc finger protein Yth1|Schizosaccharomyces pombe|chr 1|||Manual Length = 170 Score = 25.0 bits (52), Expect = 8.4 Identities = 12/33 (36%), Positives = 17/33 (51%) Frame = +3 Query: 288 IHEYYSRECREPXPCYSGHFAGWRSNGFTCICL 386 +HEY ++ PC+ GW SNG C+ L Sbjct: 71 LHEY---NLKKMPPCHFYAERGWCSNGEECLYL 100 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,609,472 Number of Sequences: 5004 Number of extensions: 56445 Number of successful extensions: 167 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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