Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV31003.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g39990.1 68415.m04914 eukaryotic translation initiation facto... 96 1e-20
At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory su... 75 4e-14
At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory su... 74 8e-14
At1g10840.1 68414.m01246 eukaryotic translation initiation facto... 49 3e-06
At5g01240.2 68418.m00032 amino acid permease, putative strong si... 30 1.0
At5g01240.1 68418.m00031 amino acid permease, putative strong si... 30 1.0
At2g46280.2 68415.m05756 eukaryotic translation initiation facto... 29 2.4
At2g46280.1 68415.m05755 eukaryotic translation initiation facto... 29 2.4
At5g22940.1 68418.m02682 exostosin family protein contains Pfam ... 28 4.1
At3g45390.1 68416.m04900 lectin protein kinase family protein co... 28 4.1
At2g28110.1 68415.m03415 exostosin family protein contains 1 tra... 27 9.5
>At2g39990.1 68415.m04914 eukaryotic translation initiation factor 3
subunit 5 / eIF-3 epsilon / eIF3f (TIF3F1) identical to
SP|O04202 Eukaryotic translation initiation factor 3
subunit 5 (eIF-3 epsilon) (eIF3 p32 subunit) (eIF3f)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 293
Score = 96.3 bits (229), Expect = 1e-20
Identities = 51/167 (30%), Positives = 87/167 (52%), Gaps = 1/167 (0%)
Frame = +1
Query: 1 ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGTS-DKGVVEVTNCFCVPHKEHADQVEA 177
++ ++HP+V+F + D + RR + RVIGTLLG+ G V++ N + VPH E +DQV
Sbjct: 26 LTARIHPLVIFNVCDCFVRRPDSAERVIGTLLGSILPDGTVDIRNSYAVPHNESSDQVAV 85
Query: 178 ELNYAMDVYELNRRVNSSESIVGWWALAMK*PTTPLLYTSIIPVNAVSLXHVTLDTSLAG 357
+++Y ++ + +VNS E+IVGW++ L + H+T+DT
Sbjct: 86 DIDYHHNMLASHLKVNSKETIVGWYSTGAGVNGGSSLIHDFYAREVPNPIHLTVDTGFTN 145
Query: 358 GRMGLRAYVCVPLGVPNGKQGCMFTPVDVTLTCYEPEIVGLQVCQKT 498
G ++A+V L + + + F + V L + E VG V + T
Sbjct: 146 GEGTIKAFVSSNLSLGDRQLVAHFQEIPVDLRMVDAERVGFDVLKAT 192
>At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26s proteasome regulatory subunit s12
(proteasome subunit p40) (mov34 protein) SP:P26516 from
[Mus musculus]; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 308
Score = 74.9 bits (176), Expect = 4e-14
Identities = 51/165 (30%), Positives = 80/165 (48%), Gaps = 6/165 (3%)
Frame = +1
Query: 7 VKVHPVVLFQIVDAYERRNADSH-RVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE--- 174
V VHP+VL IVD Y R DS RV+G LLG+S +GVV+VTN + VP +E
Sbjct: 17 VVVHPLVLLSIVDHYNRVAKDSSKRVVGVLLGSSSRGVVDVTNSYAVPFEEDDKDPSIWF 76
Query: 175 AELNYAMDVYELNRRVNSSESIVGWWALAMK*PTTPLLYTSIIPVNAVSLXHVTLDTSLA 354
+ NY ++ + +R+N+ E +VGW++ K L ++ + V +D
Sbjct: 77 LDHNYHESMFHMFKRINAKEHVVGWYSTGPKLRENDLDVHALFNGYVPNPVLVIIDVQPK 136
Query: 355 GGRMGLRAYVCVPLGVPNGKQGC--MFTPVDVTLTCYEPEIVGLQ 483
+ +AY V N Q +F V + +E E +G++
Sbjct: 137 ELGIPTKAYYAVEEVKENATQKSQKVFVHVSTEIAAHEVEEIGVE 181
>At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26S proteasome regulatory subunit S12
(MOV34) SP:P26516 from [Mus musculus]
Length = 310
Score = 73.7 bits (173), Expect = 8e-14
Identities = 51/165 (30%), Positives = 79/165 (47%), Gaps = 6/165 (3%)
Frame = +1
Query: 7 VKVHPVVLFQIVDAYERRNAD-SHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE--- 174
V VHP+VL IVD Y R D S RV+G LLG+S +G V+VTN + VP +E
Sbjct: 17 VIVHPLVLLSIVDHYNRVAKDTSKRVVGVLLGSSSRGTVDVTNSYAVPFEEDDKDTSIWF 76
Query: 175 AELNYAMDVYELNRRVNSSESIVGWWALAMK*PTTPLLYTSIIPVNAVSLXHVTLDTSLA 354
+ NY ++ + +R+N+ E IVGW++ K L ++ + V +D
Sbjct: 77 LDHNYHESMFHMFKRINAKEHIVGWYSTGPKLRENDLDVHALFNGYVPNPVLVIIDVQPK 136
Query: 355 GGRMGLRAYVCVPLGVPNGKQGC--MFTPVDVTLTCYEPEIVGLQ 483
+ +AY V N Q +F V + +E E +G++
Sbjct: 137 ELGIPTKAYYAVEEVKENATQKSQQVFVHVPTEIAAHEVEEIGVE 181
>At1g10840.1 68414.m01246 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 337
Score = 48.8 bits (111), Expect = 3e-06
Identities = 23/59 (38%), Positives = 35/59 (59%), Gaps = 1/59 (1%)
Frame = +1
Query: 79 VIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEAE-LNYAMDVYELNRRVNSSESIVGWW 252
V G LLG V+EVTNCF P ++ +++EA+ NY +++ R VN + VGW+
Sbjct: 47 VTGQLLGLDVGSVLEVTNCFPFPVRDDDEEIEADGANYQLEMMRCLREVNVDNNTVGWY 105
>At5g01240.2 68418.m00032 amino acid permease, putative strong
similarity to AUX1 GI:1531758 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 408
Score = 30.3 bits (65), Expect = 1.0
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 222 DSSVQLVNIHRVIKFRFDLIGMFFVWHAEAVGYFH 118
D++V L+ IH+ I F F ++FVW +A+G H
Sbjct: 238 DTAVILMLIHQFITFGFACTPLYFVWE-KAIGMHH 271
>At5g01240.1 68418.m00031 amino acid permease, putative strong
similarity to AUX1 GI:1531758 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 488
Score = 30.3 bits (65), Expect = 1.0
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 222 DSSVQLVNIHRVIKFRFDLIGMFFVWHAEAVGYFH 118
D++V L+ IH+ I F F ++FVW +A+G H
Sbjct: 318 DTAVILMLIHQFITFGFACTPLYFVWE-KAIGMHH 351
>At2g46280.2 68415.m05756 eukaryotic translation initiation factor 3
subunit 2 / TGF-beta receptor interacting protein 1 /
eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) identical to
eukaryotic translation initiation factor 3 subunit 2
(SP:Q38884) {Arabidopsis thaliana}; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies)
Length = 328
Score = 29.1 bits (62), Expect = 2.4
Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Frame = +1
Query: 166 QVEAELNYAMDVYELNRRVNSSESIVGWWALAMK*---PTTPLL--YTSIIPVNAVSLXH 330
Q + E+ + D+ L + + S + G K T LL YT+++PVNAVSL
Sbjct: 185 QSDEEVGHKKDITSLCKAADDSHFLTGSLDKTAKLWDMRTLTLLKTYTTVVPVNAVSLSP 244
Query: 331 VTLDTSLAGGR 363
+ L GG+
Sbjct: 245 LLNHVVLGGGQ 255
>At2g46280.1 68415.m05755 eukaryotic translation initiation factor 3
subunit 2 / TGF-beta receptor interacting protein 1 /
eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) identical to
eukaryotic translation initiation factor 3 subunit 2
(SP:Q38884) {Arabidopsis thaliana}; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies)
Length = 328
Score = 29.1 bits (62), Expect = 2.4
Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Frame = +1
Query: 166 QVEAELNYAMDVYELNRRVNSSESIVGWWALAMK*---PTTPLL--YTSIIPVNAVSLXH 330
Q + E+ + D+ L + + S + G K T LL YT+++PVNAVSL
Sbjct: 185 QSDEEVGHKKDITSLCKAADDSHFLTGSLDKTAKLWDMRTLTLLKTYTTVVPVNAVSLSP 244
Query: 331 VTLDTSLAGGR 363
+ L GG+
Sbjct: 245 LLNHVVLGGGQ 255
>At5g22940.1 68418.m02682 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 469
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +3
Query: 345 FAGWRSNGFTCI-CLCTIGSAKWKARL 422
FAG+RS + CLC +G A W RL
Sbjct: 332 FAGYRSEIVRSVFCLCPLGWAPWSPRL 358
>At3g45390.1 68416.m04900 lectin protein kinase family protein
contains Protein kinases ATP-binding region signature,
Prosite:PS00107 and Legume lectins beta-chain signature,
Prosite:PS00307
Length = 604
Score = 28.3 bits (60), Expect = 4.1
Identities = 19/69 (27%), Positives = 25/69 (36%)
Frame = -3
Query: 593 CSAGTPASSPRHHXRQVRARGHAARPATSPDTVFWHTCRPTISGS*HVRVTSTGVNMQPC 414
C++ P S P A P SPDT P + G + +TST V
Sbjct: 520 CTSIIPESRPNMEKVMQYINRDQALPDFSPDTPGIGVSTPMVMGIPGLAITSTSVTSSAS 579
Query: 413 FPFGTPNGT 387
P +P T
Sbjct: 580 VPLVSPPST 588
>At2g28110.1 68415.m03415 exostosin family protein contains 1
transmembrane domain; similar to
pectin-glucuronyltransferase (GI:23821292) [Nicotiana
plumbaginifolia]; similar to NpGUT1 homolog
(GI:23821294) [Arabidopsis thaliana]; contains Pfam
profile PF03016: Exostosin family
Length = 448
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +3
Query: 345 FAGWRSN-GFTCICLCTIGSAKWKARL 422
FAG++S + CLC +G A W RL
Sbjct: 318 FAGYQSEIARSVFCLCPLGWAPWSPRL 344
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,920,618
Number of Sequences: 28952
Number of extensions: 307654
Number of successful extensions: 807
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 797
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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