BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30995.Seq (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 140 9e-35 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 42 6e-05 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 33 0.028 SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 4.2 SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 25 5.6 SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 7.3 SPBC1604.04 |||thiamine pyrophosphate transporter|Schizosaccharo... 25 7.3 SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr ... 25 9.7 SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|S... 25 9.7 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 140 bits (340), Expect = 9e-35 Identities = 66/100 (66%), Positives = 79/100 (79%), Gaps = 2/100 (2%) Frame = +3 Query: 249 VGPPALWDLAADKQTLQNEQPLQVARCTKIINADSNDPK--YIINVKQFAKFVVDLQDSV 422 + P + WD+AAD+Q + EQPLQVARCTKII + + K Y+IN+KQ AKFVV L + V Sbjct: 74 LAPISFWDVAADRQRMSEEQPLQVARCTKIIENEQSAEKNAYVINLKQIAKFVVSLGERV 133 Query: 423 APTDIEEGMXVGVDRNKYQIHIPLPPKIDPTVTMMQVEEK 542 +PTDIEEGM VG DRNKY I +PLPPKIDP+VTMMQVEEK Sbjct: 134 SPTDIEEGMRVGCDRNKYAIQLPLPPKIDPSVTMMQVEEK 173 Score = 63.7 bits (148), Expect = 2e-11 Identities = 28/49 (57%), Positives = 36/49 (73%) Frame = +1 Query: 109 LDEGDIALLKSYGQGQYTKIIKEVEEGIQTVMKRVNELTGIKESDTGLA 255 LDEGDI LLKSY G Y + + ++E + V+KR+N+ GIKESDTGLA Sbjct: 27 LDEGDIELLKSYATGPYARELAAIKEDTEAVLKRINDTVGIKESDTGLA 75 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 41.9 bits (94), Expect = 6e-05 Identities = 19/59 (32%), Positives = 34/59 (57%) Frame = +3 Query: 363 KYIINVKQFAKFVVDLQDSVAPTDIEEGMXVGVDRNKYQIHIPLPPKIDPTVTMMQVEE 539 K ++ V K+VVD+ + +I+ + V + + YQ+ LP K+DP V++M VE+ Sbjct: 80 KVLVKVHPEGKYVVDISPDIDIKEIKPNIRVALRNDSYQLIKILPNKVDPLVSLMMVEK 138 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 33.1 bits (72), Expect = 0.028 Identities = 15/41 (36%), Positives = 24/41 (58%) Frame = +3 Query: 420 VAPTDIEEGMXVGVDRNKYQIHIPLPPKIDPTVTMMQVEEK 542 V P ++ G +GV+++ Y I LP + D V M+V+EK Sbjct: 139 VEPEELHPGDLIGVNKDSYLIIDKLPSEYDSRVKAMEVDEK 179 >SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1461 Score = 25.8 bits (54), Expect = 4.2 Identities = 12/45 (26%), Positives = 24/45 (53%), Gaps = 2/45 (4%) Frame = +3 Query: 246 RVGPPALWDLA--ADKQTLQNEQPLQVARCTKIINADSNDPKYII 374 R+G P+ L+ +D LQ+ + + + D+NDP+Y++ Sbjct: 30 RIGTPSTISLSENSDLSKLQSTNDFESREDLSLTSDDNNDPEYVM 74 >SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 25.4 bits (53), Expect = 5.6 Identities = 19/78 (24%), Positives = 34/78 (43%), Gaps = 2/78 (2%) Frame = +3 Query: 258 PALWDLAADKQTLQNEQPLQVARCTKIINADSNDPKYIINVKQFAKFV--VDLQDSVAPT 431 P W+L A + + NE + + +NAD + + V F K + D + S+ Sbjct: 227 PLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEKPSIVYA 286 Query: 432 DIEEGMXVGVDRNKYQIH 485 DI+ + + RN I+ Sbjct: 287 DIDPSV-MSTARNSVPIY 303 >SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 25.0 bits (52), Expect = 7.3 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = +1 Query: 148 QGQYTKIIKEVEEGIQTVMKRVNELTGIKESDTGLALQP 264 Q + ++K+V+EG M+RV +++ A+QP Sbjct: 326 QEEKDSLLKKVQEGASLAMQRVQNKHDLEKKRLQSAIQP 364 >SPBC1604.04 |||thiamine pyrophosphate transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 314 Score = 25.0 bits (52), Expect = 7.3 Identities = 10/39 (25%), Positives = 22/39 (56%) Frame = -3 Query: 237 LFDACQFINPLHYCLNSFFYFLNDFCVLSLAV*LQKRNI 121 L+ +C F + Y LNS FY L + + ++ + + ++ + Sbjct: 265 LWLSCHFYYSIIYLLNSMFYLLTFYSLRAITMLIYEQTL 303 >SPBC27.03 |meu25||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 622 Score = 24.6 bits (51), Expect = 9.7 Identities = 8/16 (50%), Positives = 13/16 (81%) Frame = +3 Query: 342 NADSNDPKYIINVKQF 389 N D DPK++I+V++F Sbjct: 135 NTDGYDPKFLISVRKF 150 >SPAC23H4.14 |vam6|vps39|guanyl-nucleotide exchange factor Vma6|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 24.6 bits (51), Expect = 9.7 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -2 Query: 127 QYHPRREILFLFPALLYHLSLCAYHSLNGQAF 32 +Y R ++ +FP L ++S+ +YHSL F Sbjct: 790 KYSSRLDLNRVFPLLPKNISMKSYHSLFSSQF 821 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,065,669 Number of Sequences: 5004 Number of extensions: 38012 Number of successful extensions: 125 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 124 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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