BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30990.Seq (399 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 116 1e-27 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 116 1e-27 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 0.61 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 28 0.61 SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 26 2.5 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 2.5 SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 4.3 SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|ch... 25 4.3 SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 25 4.3 SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharo... 25 5.7 SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large... 25 5.7 SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr ... 25 5.7 SPAPB1A11.03 |||FMN dependent dehydrogenase|Schizosaccharomyces ... 24 7.6 SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces ... 24 7.6 SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma... 24 7.6 SPAC2G11.04 |||RNA-binding protein, G-patch type |Schizosaccharo... 24 10.0 SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ... 24 10.0 SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 24 10.0 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 116 bits (280), Expect = 1e-27 Identities = 53/75 (70%), Positives = 62/75 (82%) Frame = +1 Query: 31 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 210 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 211 DTGNFSWGSECSTRK 255 D+GNFSWGSE ++K Sbjct: 61 DSGNFSWGSEGVSKK 75 Score = 71.7 bits (168), Expect = 4e-14 Identities = 31/50 (62%), Positives = 39/50 (78%) Frame = +3 Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLG 395 + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G Sbjct: 73 SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 116 bits (280), Expect = 1e-27 Identities = 53/75 (70%), Positives = 62/75 (82%) Frame = +1 Query: 31 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 210 MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 211 DTGNFSWGSECSTRK 255 D+GNFSWGSE ++K Sbjct: 61 DSGNFSWGSEGVSKK 75 Score = 71.7 bits (168), Expect = 4e-14 Identities = 31/50 (62%), Positives = 39/50 (78%) Frame = +3 Query: 246 NSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLG 395 + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY + +G Sbjct: 73 SKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG 122 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.9 bits (59), Expect = 0.61 Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 2/74 (2%) Frame = +1 Query: 43 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 216 RD +K + G K+ + EL +N + Q+ + S T YRA+ LDT Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142 Query: 217 GNFSWGSECSTRKP 258 G+ E + KP Sbjct: 1143 GDLMAVKEVALHKP 1156 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 27.9 bits (59), Expect = 0.61 Identities = 17/54 (31%), Positives = 26/54 (48%) Frame = +1 Query: 46 DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 207 + W K T K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPAC139.03 |||transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 625 Score = 25.8 bits (54), Expect = 2.5 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = +1 Query: 19 DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 135 + TK G + RRA A RKK K + GRP +N Sbjct: 3 ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 2.5 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -3 Query: 367 YHCLNGVASTTTIAFLTRVFVRTNS 293 ++C N V S T + + VFVR NS Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542 >SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 689 Score = 25.0 bits (52), Expect = 4.3 Identities = 12/60 (20%), Positives = 29/60 (48%), Gaps = 7/60 (11%) Frame = -2 Query: 338 YNNCILDKGLCTHQFIVRCIIH-------NINDTGLRVEHSDPQEKLPVSRRSARYLVFP 180 + N + + +H FI R +I+ +I D G ++ + ++ L + + Y+++P Sbjct: 540 FENSLYHQSFSSHWFISRLLINPIRMLCWHITDVGKTLDLGEAEKLLKYNHKQCPYIIYP 599 >SPBC530.05 |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.0 bits (52), Expect = 4.3 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = -3 Query: 394 PRGNV*CDSYHCLNGVASTTTIAFLTRVFVRTNSLLDAL 278 P ++ + +CL V + I+F T FVR N ++DAL Sbjct: 544 PLASICSEDLNCLISVKNRL-ISFETHGFVRLNVVMDAL 581 >SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 25.0 bits (52), Expect = 4.3 Identities = 8/23 (34%), Positives = 14/23 (60%) Frame = +1 Query: 46 DHWHKRRATGGKRAPIRKKRKYE 114 D W +A G +PIR+ ++Y+ Sbjct: 758 DEWKASKAFGANDSPIRENKRYK 780 >SPBC530.01 |gyp1||GTPase activating protein Gyp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 24.6 bits (51), Expect = 5.7 Identities = 12/27 (44%), Positives = 15/27 (55%), Gaps = 1/27 (3%) Frame = +1 Query: 19 DP-TKMGISRDHWHKRRATGGKRAPIR 96 DP T + SR H H+ R+T K P R Sbjct: 27 DPVTVLDRSRTHHHRGRSTSSKAQPFR 53 >SPBC651.08c |rpc1||DNA-directed RNA polymerase III complex large subunit Rpc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1405 Score = 24.6 bits (51), Expect = 5.7 Identities = 8/19 (42%), Positives = 14/19 (73%) Frame = +2 Query: 311 DPCQECNCCSRCNSIQAVV 367 D C++ CS+C+++Q VV Sbjct: 145 DHCKKMRRCSKCDAMQGVV 163 >SPBC16H5.13 |||WD repeat protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1026 Score = 24.6 bits (51), Expect = 5.7 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%) Frame = +1 Query: 214 TGNFSWGSECST--RKPVSLMLCIMHLTMNWCVQ 309 +G+F W S C+ KPVSL + +T NW + Sbjct: 282 SGDFPWKS-CTILKSKPVSLCVYPEKITFNWLTE 314 >SPAPB1A11.03 |||FMN dependent dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 24.2 bits (50), Expect = 7.6 Identities = 9/12 (75%), Positives = 11/12 (91%) Frame = +1 Query: 361 SGMSRIIRCLLG 396 SG+S +IRCLLG Sbjct: 368 SGVSHVIRCLLG 379 >SPAC6B12.15 |cpc2|rkp1|RACK1 homologue Cpc2|Schizosaccharomyces pombe|chr 1|||Manual Length = 314 Score = 24.2 bits (50), Expect = 7.6 Identities = 8/11 (72%), Positives = 10/11 (90%) Frame = -2 Query: 323 LDKGLCTHQFI 291 L+KG CTHQF+ Sbjct: 92 LEKGECTHQFV 102 >SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Manual Length = 835 Score = 24.2 bits (50), Expect = 7.6 Identities = 16/52 (30%), Positives = 27/52 (51%), Gaps = 5/52 (9%) Frame = +2 Query: 125 PLQTPGSALSESTP-FVHV----VEILSTVRCVWTPVTSLGDRNVQLANPYH 265 PL+ E++P FV V +E+L + V ++GDR +Q+ + YH Sbjct: 123 PLEEEKLYAPENSPGFVEVLEGEIELLPNLPRTPIEVRNMGDRPIQVGSHYH 174 >SPAC2G11.04 |||RNA-binding protein, G-patch type |Schizosaccharomyces pombe|chr 1|||Manual Length = 301 Score = 23.8 bits (49), Expect = 10.0 Identities = 9/22 (40%), Positives = 10/22 (45%) Frame = +2 Query: 200 RCVWTPVTSLGDRNVQLANPYH 265 RC W LG N + NP H Sbjct: 262 RCGWKEGQGLGQHNQGIINPLH 283 >SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1337 Score = 23.8 bits (49), Expect = 10.0 Identities = 9/31 (29%), Positives = 21/31 (67%) Frame = -2 Query: 161 WIR*GPSLVFAAGRPNSYFLFLRMGARFPPV 69 ++R PS+++A ++ LF+R+ ++PP+ Sbjct: 327 FVRPLPSIIWALFEFSAVALFIRLKMKYPPL 357 >SPAPB1A10.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 23.8 bits (49), Expect = 10.0 Identities = 9/24 (37%), Positives = 12/24 (50%) Frame = -1 Query: 81 FPTGGPSLMPVIPTNTHLSWVKKE 10 +PT L+ PT H SW +E Sbjct: 190 YPTDSKQLVKYHPTEVHPSWTVEE 213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,861,848 Number of Sequences: 5004 Number of extensions: 39251 Number of successful extensions: 116 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 116 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 134126124 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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