BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30972.Seq (597 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosacc... 31 0.17 SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces pom... 27 2.1 SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 26 3.6 SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 25 6.3 SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 6.3 SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 25 6.3 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 8.4 SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1... 25 8.4 SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 25 8.4 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.4 >SPBC4.04c |mcm2|cdc19, nda1|MCM complex subunit Mcm2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 830 Score = 30.7 bits (66), Expect = 0.17 Identities = 20/56 (35%), Positives = 26/56 (46%) Frame = -3 Query: 586 DARLXGRDGCRLRAP*GNSSPTEGWRELSSDADAG*FTGYTSHSSPHDGKYSPGGG 419 DARL RD L A G + P ++ D D+ TG+T H +YSP G Sbjct: 112 DARLRRRD-IELDAAAGRTKPAAFLQDEDDDLDSNLGTGFTRHRHRIYDEYSPNVG 166 >SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 158 Score = 27.1 bits (57), Expect = 2.1 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = -1 Query: 471 GIPVIRPLMMESTALGAAIVAGRAMRVW 388 GIP+I P+ L A+ + RA ++W Sbjct: 131 GIPIIDPVTRAPAVLAGAVSSSRAKQMW 158 >SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 26.2 bits (55), Expect = 3.6 Identities = 16/43 (37%), Positives = 19/43 (44%) Frame = -1 Query: 375 PSPPADTFLPALTNXXXXXXXXXXXEALNKCMGWTDTKNELST 247 P PP+ T LPAL + E T+TK ELST Sbjct: 61 PRPPSATSLPALLSLFQEEWDSVALEQFELRRNLTETKQELST 103 >SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces pombe|chr 2|||Manual Length = 897 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/32 (31%), Positives = 16/32 (50%) Frame = -1 Query: 276 WTDTKNELSTQKNQIELLQFCRRDFFSSEPPY 181 W +T+N + N +E+L R+ S P Y Sbjct: 764 WANTENARYSTSNALEILDMLLREKIESAPRY 795 >SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit Sec63 |Schizosaccharomyces pombe|chr 2|||Manual Length = 611 Score = 25.4 bits (53), Expect = 6.3 Identities = 15/63 (23%), Positives = 30/63 (47%) Frame = -1 Query: 528 RRRRDGANSVLMQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPTTIPSPPADTFL 349 R++ + N+ + Q+D+ V+ L+ +TA G A +++W + + P D Sbjct: 281 RKKTNNFNTHQILSQSDV----VLNALLSVATAFGFANPVDNVLKLWQHIVQAIPLDAPF 336 Query: 348 PAL 340 P L Sbjct: 337 PLL 339 >SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 1616 Score = 25.4 bits (53), Expect = 6.3 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = -2 Query: 287 NAWDGPILKMNCQRRRIR*NYCSFAAGTFFPRNRPTSD 174 N W P L++NC + Y + G+F+ + R T D Sbjct: 1434 NLWK-PHLQLNCLSDEVAQYYITLCKGSFYYQMRNTED 1470 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 25.0 bits (52), Expect = 8.4 Identities = 9/21 (42%), Positives = 12/21 (57%) Frame = +1 Query: 349 QECIGRWAGYCRGPHTHCATC 411 QEC+ W G+ + THC C Sbjct: 36 QECLVEWLGHSK--KTHCELC 54 >SPAC1142.08 |fhl1|SPAC8C9.01|fork head transcription factor Fhl1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 743 Score = 25.0 bits (52), Expect = 8.4 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +3 Query: 471 PVNQPASASELSSRHPSVGEELPQGA 548 P +P++ S+L S+G+ LP GA Sbjct: 584 PYVRPSAPSKLPDTRQSIGDPLPPGA 609 >SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 741 Score = 25.0 bits (52), Expect = 8.4 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +1 Query: 274 PSHAFIQGFFPTNSPHFSFF-IGKSRQECIGRWAGYCRGPHT 396 PS + Q F+P S S + +GK+ Q G + Y G T Sbjct: 410 PSTVYHQVFYPPTSTSVSSYGLGKTPQPAYGSSSPYVNGHQT 451 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 25.0 bits (52), Expect = 8.4 Identities = 9/23 (39%), Positives = 13/23 (56%) Frame = +3 Query: 372 WVLSWATHALRDLRQWPPPGLYF 440 W L+ A D+R WPP ++F Sbjct: 403 WTLTDLADAFLDVRLWPPIFMFF 425 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,369,366 Number of Sequences: 5004 Number of extensions: 49609 Number of successful extensions: 131 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 131 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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