Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30963.Seq
(548 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 62 4e-11
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 51 1e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 49 4e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 40 2e-04
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 39 4e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 36 0.003
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.004
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 31 0.15
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.0
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 3.2
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 25 5.6
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 25 7.3
SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase Alg11|Schiz... 25 7.3
SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces po... 25 7.3
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 62.5 bits (145), Expect = 4e-11
Identities = 32/84 (38%), Positives = 50/84 (59%), Gaps = 2/84 (2%)
Frame = +1
Query: 262 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAV 438
I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V
Sbjct: 73 ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTV 131
Query: 439 EVTSAEQAKELID-ANXVIVFGFF 507
+ S + + ++ A+ + V FF
Sbjct: 132 KPISKDTLENFVEKADDLAVVAFF 155
Score = 60.1 bits (139), Expect = 2e-10
Identities = 24/53 (45%), Positives = 39/53 (73%), Gaps = 1/53 (1%)
Frame = +2
Query: 98 TEENVLXLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAEE 253
++E+++ L NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + +++
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD 405
Score = 59.3 bits (137), Expect = 4e-10
Identities = 22/47 (46%), Positives = 34/47 (72%)
Frame = +2
Query: 119 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEDL 259
++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L ++ +
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGI 73
Score = 37.9 bits (84), Expect = 0.001
Identities = 20/57 (35%), Positives = 36/57 (63%), Gaps = 3/57 (5%)
Frame = +1
Query: 256 SPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 417
S + +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ +++ K
Sbjct: 406 SNVVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 51.2 bits (117), Expect = 1e-07
Identities = 24/67 (35%), Positives = 31/67 (46%)
Frame = +2
Query: 44 LIFTAIALLGLALGDEVPTEENVLXLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 223
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 224 AKAATKL 244
K A+ L
Sbjct: 71 QKLASNL 77
Score = 30.3 bits (65), Expect = 0.20
Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%)
Frame = +1
Query: 259 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 420
P+ DA Q + + Y V+G+PT+K GS + DY+G R + ++
Sbjct: 82 PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141
Query: 421 TGPPAVEVTSAE 456
P V++ ++E
Sbjct: 142 I-PSKVKILTSE 152
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 49.2 bits (112), Expect = 4e-07
Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +2
Query: 107 NVLXLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 223
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 48.0 bits (109), Expect = 9e-07
Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 2/76 (2%)
Frame = +1
Query: 262 IKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLKKKTGPPA 435
+ + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ +KTG
Sbjct: 74 VLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKK 133
Query: 436 VEVTSAEQAKELIDAN 483
++ EL N
Sbjct: 134 RKIVLPSNVVELDSLN 149
Score = 46.8 bits (106), Expect = 2e-06
Identities = 24/65 (36%), Positives = 34/65 (52%)
Frame = +2
Query: 29 IEMRVLIFTAIALLGLALGDEVPTEENVLXLSKANFETVITTTEYILVEFYAPWCGHCKS 208
+ + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56
Query: 209 LAPEY 223
LAP Y
Sbjct: 57 LAPVY 61
Score = 37.1 bits (82), Expect = 0.002
Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 3/58 (5%)
Frame = +1
Query: 262 IKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 426
+++ K++A D+ + V +PT+KFF P Y G R + +I ++ KK+G
Sbjct: 193 VEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 40.3 bits (90), Expect = 2e-04
Identities = 11/32 (34%), Positives = 25/32 (78%)
Frame = +2
Query: 128 ANFETVITTTEYILVEFYAPWCGHCKSLAPEY 223
+ F++++ + ++V+F+A WCG CK++AP++
Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF 40
Score = 27.1 bits (57), Expect = 1.8
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +1
Query: 274 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 369
KVD Q ++A GV P+ ++NG I+
Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.1 bits (87), Expect = 4e-04
Identities = 14/36 (38%), Positives = 25/36 (69%)
Frame = +2
Query: 140 TVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLA 247
+ I + Y+ V+ YA WCG CK+++P +++ A+K A
Sbjct: 14 STIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYA 49
Score = 35.9 bits (79), Expect = 0.004
Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%)
Frame = +1
Query: 271 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 438
AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG A+
Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115
Query: 439 EVTSAEQAK 465
+S+ K
Sbjct: 116 ASSSSAPVK 124
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 36.3 bits (80), Expect = 0.003
Identities = 12/65 (18%), Positives = 36/65 (55%)
Frame = +1
Query: 262 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 441
+K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289
Query: 442 VTSAE 456
+ +
Sbjct: 290 IEEGD 294
Score = 28.3 bits (60), Expect = 0.79
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +1
Query: 301 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 447
LA YG + P++ RNG PI Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 35.9 bits (79), Expect = 0.004
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = +2
Query: 122 SKANFETVITTTEYILVEFYAPWCGHCKSLAP 217
S ++ T I+ + +V+FYA WCG CK L P
Sbjct: 24 SFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55
Score = 31.1 bits (67), Expect = 0.11
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 277 VDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 417
V+A + D+A+ GV PT+ FR G +D G + S L K
Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 30.7 bits (66), Expect = 0.15
Identities = 11/55 (20%), Positives = 29/55 (52%)
Frame = +1
Query: 253 RSPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 417
R + +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++
Sbjct: 328 RGKLNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
Score = 28.3 bits (60), Expect = 0.79
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +2
Query: 170 VEFYAPWCGHCKSLAPEYAKAATKLAEE 253
+++Y P CG CK L P + K E+
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQ 74
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 27.9 bits (59), Expect = 1.0
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2
Query: 158 EYILVEFYAPWCGHCKSL 211
+ IL+ FYAPW CK +
Sbjct: 21 QIILLNFYAPWAAPCKQM 38
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 26.2 bits (55), Expect = 3.2
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 147 LQPRSTF*LNSMLHGAATANLWHRNTP 227
LQP T N +L+ + NLW R+ P
Sbjct: 634 LQPTITLGKNDLLNAILSQNLWFRSLP 660
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 25.4 bits (53), Expect = 5.6
Identities = 12/45 (26%), Positives = 22/45 (48%)
Frame = +3
Query: 201 ANLWHRNTPRQQQSWLKKISYQTSES*RNSRTGSRRELRCTRIPD 335
AN + + QSW +++ + +E+ S S R + T IP+
Sbjct: 74 ANSFEGSCNNSDQSWTSRVTSKKNEAGTESGDASVRRIYVTSIPE 118
>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1052
Score = 25.0 bits (52), Expect = 7.3
Identities = 20/62 (32%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +2
Query: 218 EYAKAATKLAEEDLLSN*RKL-TQLKNRISPRATVYEDTRLSNSSGMAVLSTIQVVVKLM 394
E K A KL E+DLLS + Q + +I + R N +A+ + ++ VVK M
Sbjct: 457 ENEKKAAKLIEKDLLSQISHVKLQFEQQIKAHHYAKDQVRNLNDEMVALRAELE-VVKAM 515
Query: 395 TS 400
S
Sbjct: 516 NS 517
>SPCC330.08 |alg11|gmd3|alpha-1,2-mannosyltransferase
Alg11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 471
Score = 25.0 bits (52), Expect = 7.3
Identities = 11/33 (33%), Positives = 19/33 (57%)
Frame = +1
Query: 364 IDYSGGRQADDIISWLKKKTGPPAVEVTSAEQA 462
++ SGG + D +I W+ K TG A ++ +A
Sbjct: 389 VNNSGGPKFDIVIPWIGKPTGFHASTISEYAEA 421
>SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 742
Score = 25.0 bits (52), Expect = 7.3
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = -3
Query: 465 FSLFSRGDLNSRGASLLLQPTDDVISLTTT*IVDRT 358
F S+G+L+ SLL +D+V +TTT +VD T
Sbjct: 304 FEFDSKGNLD---VSLLKSLSDEVNMITTTSLVDNT 336
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,091,666
Number of Sequences: 5004
Number of extensions: 38958
Number of successful extensions: 133
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 132
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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