Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30946.Seq
         (558 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo...    84   2e-17
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo...    59   4e-10
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    27   2.5  
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc...    26   4.3  
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr...    25   5.7  
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar...    25   10.0 

>SPBC4C3.07 |||translation initiation factor
           eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 302

 Score = 83.8 bits (198), Expect = 2e-17
 Identities = 37/87 (42%), Positives = 57/87 (65%), Gaps = 2/87 (2%)
 Frame = +1

Query: 1   ISVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVE 174
           +++ + P VLF I+D   R++ ++ RVIGTLLGT   D   +E+ +CF VPH E ++QVE
Sbjct: 21  LNIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVE 80

Query: 175 AELNYAMDVYELNRRVNSSESIVGWWA 255
            E+ Y   +Y L+ + N  E +VGW+A
Sbjct: 81  VEMEYHRAMYHLHLKANPREVVVGWYA 107


>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
           Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 324

 Score = 59.3 bits (137), Expect = 4e-10
 Identities = 29/86 (33%), Positives = 48/86 (55%), Gaps = 4/86 (4%)
 Frame = +1

Query: 7   VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 177
           V VHP+VL   VD+Y R       RV+G LLG ++  VV V N + +P +E         
Sbjct: 17  VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76

Query: 178 -ELNYAMDVYELNRRVNSSESIVGWW 252
            + N+   + E+ +++N++E +VGW+
Sbjct: 77  LDHNFMESMNEMFKKINANEKLVGWY 102


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 26.6 bits (56), Expect = 2.5
 Identities = 13/42 (30%), Positives = 21/42 (50%)
 Frame = +1

Query: 49  YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 174
           Y+ R  DSHR I  L GT  +  +   +   + +K H  ++E
Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524


>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
           Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 596

 Score = 25.8 bits (54), Expect = 4.3
 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = +3

Query: 60  KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 203
           + R S SY   I  ++  SGG + + L  A  RTC  S + T L + CL
Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247


>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 25.4 bits (53), Expect = 5.7
 Identities = 6/11 (54%), Positives = 10/11 (90%)
 Frame = +2

Query: 422 HVHSCRCHSHM 454
           H+H CRCH+++
Sbjct: 69  HIHCCRCHTYI 79


>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
           subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1689

 Score = 24.6 bits (51), Expect = 10.0
 Identities = 10/28 (35%), Positives = 14/28 (50%)
 Frame = -3

Query: 397 PNGTXTYARKPIRPPASEVSRVXWTGSR 314
           P+ T  Y R    PPA +   + WTG +
Sbjct: 718 PDETGMYGRIKTLPPAIQRPGIYWTGKQ 745


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,404,380
Number of Sequences: 5004
Number of extensions: 49386
Number of successful extensions: 133
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)