Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30936.Seq
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy...   113   2e-26
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar...   104   1e-23
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar...    66   3e-12
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo...    51   1e-07
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||...    36   0.007
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|...    34   0.022
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit...    33   0.052
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||...    33   0.052
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||...    28   1.1  
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||...    28   1.1  
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ...    26   4.5  
SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch...    26   4.5  
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9...    26   6.0  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   6.0  

>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 180

 Score =  113 bits (272), Expect = 2e-26
 Identities = 59/132 (44%), Positives = 76/132 (57%)
 Frame = +3

Query: 255 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 434
           YKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ  IRP WR Y+ NT  II+VVDS
Sbjct: 35  YKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDS 94

Query: 435 ADRXRIGISKDELVHMLRXXXXXXXXXXXXXXKQXMGRMSDSSRGTPGPWGLDAXRDRTF 614
            DR RI  + +EL  ML               KQ +    +++  T    GL + R R +
Sbjct: 95  NDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEIT-DKLGLHSLRHRQW 153

Query: 615 QXFKTSAVRSEG 650
               T A   +G
Sbjct: 154 YIQATCATSGDG 165



 Score = 43.6 bits (98), Expect = 3e-05
 Identities = 20/30 (66%), Positives = 23/30 (76%)
 Frame = +1

Query: 178 FRGLLGAREMRILILGLDGAGKTTILINCK 267
           F+ L G REMRIL++GLD AGKTTIL   K
Sbjct: 9   FQSLFGKREMRILMVGLDAAGKTTILYKLK 38


>SPBC1539.08 |||ADP-ribosylation factor, Arf
           family|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score =  104 bits (249), Expect = 1e-23
 Identities = 55/133 (41%), Positives = 75/133 (56%), Gaps = 1/133 (0%)
 Frame = +3

Query: 255 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 434
           YKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ  IRP WR Y+  T  +I+VVDS
Sbjct: 39  YKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDS 98

Query: 435 ADRXRIGISKDELVHMLRXXXXXXXXXXXXXXKQXM-GRMSDSSRGTPGPWGLDAXRDRT 611
           AD  RI  ++ EL  ++               KQ + G +S +         LD  +DR 
Sbjct: 99  ADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQ--ITDVLQLDKLKDRL 156

Query: 612 FQXFKTSAVRSEG 650
           +    T A+  +G
Sbjct: 157 WNVQPTCALTGDG 169



 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 20/35 (57%), Positives = 22/35 (62%)
 Frame = +1

Query: 163 GLFSYFRGLLGAREMRILILGLDGAGKTTILINCK 267
           G    F  L   +EMRIL+LGLD AGKTTIL   K
Sbjct: 8   GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42


>SPAC22F3.05c |alp41||ADP-ribosylation factor
           Alp41|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 186

 Score = 66.5 bits (155), Expect = 3e-12
 Identities = 26/65 (40%), Positives = 44/65 (67%)
 Frame = +3

Query: 291 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRXRIGISKDE 470
           PT GF +  +  + L+F +WD+GGQ ++R +W+ Y+ +T+AII+VVDS D  R+   ++ 
Sbjct: 46  PTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNT 105

Query: 471 LVHML 485
           L  +L
Sbjct: 106 LQELL 110



 Score = 35.9 bits (79), Expect = 0.006
 Identities = 16/22 (72%), Positives = 19/22 (86%)
 Frame = +1

Query: 190 LGAREMRILILGLDGAGKTTIL 255
           L  RE+R+L+LGLD AGKTTIL
Sbjct: 12  LKEREVRVLLLGLDNAGKTTIL 33


>SPBC31F10.06c |sar1||ADP-ribosylation factor
           Sar1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 190

 Score = 51.2 bits (117), Expect = 1e-07
 Identities = 24/65 (36%), Positives = 36/65 (55%)
 Frame = +3

Query: 291 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRXRIGISKDE 470
           PT+    E++   N++F  +DLGG    R  WR Y+   + I+Y+VD  D  R+  SK E
Sbjct: 50  PTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAE 109

Query: 471 LVHML 485
           L  +L
Sbjct: 110 LDALL 114



 Score = 28.7 bits (61), Expect = 0.85
 Identities = 13/26 (50%), Positives = 18/26 (69%)
 Frame = +1

Query: 199 REMRILILGLDGAGKTTILINCKSVR 276
           +  ++L LGLD AGKTT+L   K+ R
Sbjct: 19  KHAKMLFLGLDNAGKTTLLHMLKNDR 44


>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 203

 Score = 35.5 bits (78), Expect = 0.007
 Identities = 20/56 (35%), Positives = 26/56 (46%), Gaps = 4/56 (7%)
 Frame = +3

Query: 288 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADR 443
           I TIG + +  T+    K +K Q+WD  GQ   R     YY     II V D  D+
Sbjct: 38  ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQ 93


>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 200

 Score = 33.9 bits (74), Expect = 0.022
 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%)
 Frame = +3

Query: 279 VTTIPTIGFNVEQVTY--KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADR 443
           +TTI  I F +  +    K +K Q+WD  GQ   R     YY     I+ + D  D+
Sbjct: 39  ITTIG-IDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLLYDVTDK 94


>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
           Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 354

 Score = 32.7 bits (71), Expect = 0.052
 Identities = 15/49 (30%), Positives = 28/49 (57%)
 Frame = +3

Query: 294 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSAD 440
           T+G +    T  +L+ +++D+GGQ + R  W   + N ++II+ V   D
Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLND 227


>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 234

 Score = 32.7 bits (71), Expect = 0.052
 Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
 Frame = +3

Query: 246 DNSYKLQVG-EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIY 422
           D+     VG +  + I ++G   +Q   K +K Q+WD  GQ   R   R YY      + 
Sbjct: 35  DDQVSHTVGIDFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVL 91

Query: 423 VVDSADR 443
           V D  ++
Sbjct: 92  VYDVTNK 98


>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 211

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 11/34 (32%), Positives = 17/34 (50%)
 Frame = +3

Query: 330 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 431
           ++K ++WD  GQ   +     YY N +  I V D
Sbjct: 63  SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYD 96


>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 214

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 12/35 (34%), Positives = 15/35 (42%)
 Frame = +3

Query: 327 KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 431
           K +K Q+WD  GQ   R     YY      + V D
Sbjct: 57  KKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYD 91


>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 407

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 9/21 (42%), Positives = 16/21 (76%)
 Frame = +1

Query: 193 GAREMRILILGLDGAGKTTIL 255
           G  ++++L+LG   +GKTTI+
Sbjct: 71  GGNDIKVLLLGAGDSGKTTIM 91


>SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 198

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 13/59 (22%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
 Frame = +3

Query: 297 IGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRXRIGISKD 467
           + F  + ++ +N  + F +WDLGGQ            +  AI+++ D + +  +   K+
Sbjct: 45  VNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKE 103


>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
            Srb9|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1223

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = -1

Query: 296  RRYSGHDLTDLQFIRIVVFPAPSNPSIKIRISLA 195
            R  S H+L+ + FIR +  P P++   KI   LA
Sbjct: 1107 RNNSSHELSLVAFIREMAMPVPNDEFKKISTILA 1140


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 23/88 (26%), Positives = 33/88 (37%), Gaps = 3/88 (3%)
 Frame = +3

Query: 195 CKGNADFDTWIGRCRKDDNSYKLQVGEVV--TTIPTIGFNVEQVT-YKNLKFQVWDLGGQ 365
           C+ N  F  W+  CR D    +L     +   T     + +E  T  K+ +   WD    
Sbjct: 581 CQRNTYFARWLAACRSDPRCRRLDFQHFLQRPTQRLQRYTLELDTILKHTEQSSWDFQLI 640

Query: 366 TSIRPYWRCYYGNTDAIIYVVDSADRXR 449
           T      R      DA+I  V  A+R R
Sbjct: 641 TQAVKELRATCEECDAVIATVLEANRIR 668


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,734,293
Number of Sequences: 5004
Number of extensions: 55406
Number of successful extensions: 158
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)