BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30934.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 62 6e-11 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 48 1e-06 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 44 3e-05 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 40 5e-04 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 36 0.004 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 36 0.004 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.006 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 30 0.37 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 1.5 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.0 SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces pomb... 27 3.4 SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.4 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 7.9 SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 7.9 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 62.5 bits (145), Expect = 6e-11 Identities = 32/84 (38%), Positives = 50/84 (59%), Gaps = 2/84 (2%) Frame = +2 Query: 260 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAV 436 I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V Sbjct: 73 ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTV 131 Query: 437 EVTSAEQAKELID-ANTVIVFGFF 505 + S + + ++ A+ + V FF Sbjct: 132 KPISKDTLENFVEKADDLAVVAFF 155 Score = 55.2 bits (127), Expect = 9e-09 Identities = 22/41 (53%), Positives = 33/41 (80%), Gaps = 1/41 (2%) Frame = +3 Query: 96 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAP 215 ++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAP 393 Score = 48.8 bits (111), Expect = 7e-07 Identities = 17/33 (51%), Positives = 25/33 (75%) Frame = +3 Query: 117 LSKANFETVITTTEYILVEFYAPWCGHCKSLAP 215 ++K +IT + ++V+FYAPWCGHCK+LAP Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAP 59 Score = 37.1 bits (82), Expect = 0.002 Identities = 19/55 (34%), Positives = 35/55 (63%), Gaps = 3/55 (5%) Frame = +2 Query: 260 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 415 + +AK+DAT E D++ S + G+PT+ FF+ +P+ Y G R +D+ +++ K Sbjct: 408 VVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 48.0 bits (109), Expect = 1e-06 Identities = 24/76 (31%), Positives = 40/76 (52%), Gaps = 2/76 (2%) Frame = +2 Query: 260 IKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGGRQADDIISWLKKKTGPPA 433 + + K+DA D+A+ Y + G+PTL +F +GS P+ YS R D + ++ +KTG Sbjct: 74 VLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKK 133 Query: 434 VEVTSAEQAKELIDAN 481 ++ EL N Sbjct: 134 RKIVLPSNVVELDSLN 149 Score = 46.4 bits (105), Expect = 4e-06 Identities = 20/38 (52%), Positives = 26/38 (68%), Gaps = 1/38 (2%) Frame = +3 Query: 105 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAP 215 NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAP 178 Score = 44.4 bits (100), Expect = 2e-05 Identities = 23/63 (36%), Positives = 33/63 (52%) Frame = +3 Query: 27 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 206 + + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56 Query: 207 LAP 215 LAP Sbjct: 57 LAP 59 Score = 37.1 bits (82), Expect = 0.002 Identities = 17/58 (29%), Positives = 31/58 (53%), Gaps = 3/58 (5%) Frame = +2 Query: 260 IKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 424 +++ K++A D+ + V +PT+KFF P Y G R + +I ++ KK+G Sbjct: 193 VEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 43.6 bits (98), Expect = 3e-05 Identities = 20/58 (34%), Positives = 26/58 (44%) Frame = +3 Query: 42 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 215 L +L+ G N + L+ NF + LV FYAPWCG+CK L P Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVP 68 Score = 30.3 bits (65), Expect = 0.28 Identities = 19/72 (26%), Positives = 34/72 (47%), Gaps = 6/72 (8%) Frame = +2 Query: 257 PIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGRQADDIISWLKKK 418 P+ DA Q + + Y V+G+PT+K GS + DY+G R + ++ Sbjct: 82 PVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDS 141 Query: 419 TGPPAVEVTSAE 454 P V++ ++E Sbjct: 142 I-PSKVKILTSE 152 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 39.5 bits (88), Expect = 5e-04 Identities = 12/34 (35%), Positives = 24/34 (70%) Frame = +3 Query: 114 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 215 V + F++++ + ++V+F+A WCG CK++AP Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAP 38 Score = 27.1 bits (57), Expect = 2.6 Identities = 11/32 (34%), Positives = 17/32 (53%) Frame = +2 Query: 272 KVDATQEQDLAESYGVRGYPTLKFFRNGSPID 367 KVD Q ++A GV P+ ++NG I+ Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 36.3 bits (80), Expect = 0.004 Identities = 12/65 (18%), Positives = 36/65 (55%) Frame = +2 Query: 260 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVE 439 +K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++ Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289 Query: 440 VTSAE 454 + + Sbjct: 290 IEEGD 294 Score = 28.3 bits (60), Expect = 1.1 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%) Frame = +2 Query: 299 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 445 LA YG + P++ RNG PI Y R+ D I W+ + P E+T Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 36.3 bits (80), Expect = 0.004 Identities = 15/34 (44%), Positives = 21/34 (61%) Frame = +3 Query: 114 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 215 V S ++ T I+ + +V+FYA WCG CK L P Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP 55 Score = 31.1 bits (67), Expect = 0.16 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +2 Query: 275 VDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 415 V+A + D+A+ GV PT+ FR G +D G + S L K Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 35.9 bits (79), Expect = 0.006 Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 4/69 (5%) Frame = +2 Query: 269 AKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAV 436 AKV+ +++ +A GV+ PT FF NG ID G QA + + K TG A+ Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGAL 115 Query: 437 EVTSAEQAK 463 +S+ K Sbjct: 116 ASSSSAPVK 124 Score = 33.9 bits (74), Expect = 0.022 Identities = 12/34 (35%), Positives = 22/34 (64%) Frame = +3 Query: 114 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAP 215 + S ++ + I + Y+ V+ YA WCG CK+++P Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISP 39 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.9 bits (64), Expect = 0.37 Identities = 11/55 (20%), Positives = 29/55 (52%) Frame = +2 Query: 251 RTPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 415 R + +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ Sbjct: 328 RGKLNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382 Score = 25.8 bits (54), Expect = 6.0 Identities = 8/16 (50%), Positives = 11/16 (68%) Frame = +3 Query: 168 VEFYAPWCGHCKSLAP 215 +++Y P CG CK L P Sbjct: 47 IKYYLPSCGACKRLGP 62 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 27.9 bits (59), Expect = 1.5 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +3 Query: 156 EYILVEFYAPWCGHCKSL 209 + IL+ FYAPW CK + Sbjct: 21 QIILLNFYAPWAAPCKQM 38 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/41 (34%), Positives = 19/41 (46%) Frame = +1 Query: 103 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 225 K +F L LQP T N +L+ + NLW R+ P Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660 >SPBC106.19 ||SPBC582.01|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 515 Score = 26.6 bits (56), Expect = 3.4 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -3 Query: 657 FSSKVLEKHNIFIFSLQLLDHF 592 F+SK+ HNI FSL L+ F Sbjct: 455 FASKIKPSHNIISFSLSLVKRF 476 >SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 113 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/33 (39%), Positives = 16/33 (48%) Frame = -2 Query: 568 LGHQQLEQLKGKFWLWLSSGRKETKYNNSIGID 470 +G E++ GKF W RK TK GID Sbjct: 43 IGRDTAEKVNGKFESWDGLFRKSTKTMKKEGID 75 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/34 (38%), Positives = 18/34 (52%) Frame = -3 Query: 681 SSSSYLTRFSSKVLEKHNIFIFSLQLLDHFLIAD 580 S YLT S+ KHN+ +F+ L+D L D Sbjct: 1935 SPLQYLTCESTSTSWKHNLLVFARNLMDGSLQND 1968 >SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1379 Score = 25.4 bits (53), Expect = 7.9 Identities = 11/35 (31%), Positives = 18/35 (51%) Frame = +2 Query: 428 PAVEVTSAEQAKELIDANTVIVFGFFSTRAQPEPK 532 PAVE +K+ +A ++ F FF+ +P K Sbjct: 219 PAVEKLKTSASKQAAEAQSLREFDFFTPTPEPSDK 253 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,597,622 Number of Sequences: 5004 Number of extensions: 49318 Number of successful extensions: 160 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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