Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30871.Seq
         (499 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC31A2.02 |trm112||tRNA |Schizosaccharomyces pombe|chr 1|||Manual    40   3e-04
SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces pom...    31   0.13 
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    30   0.22 
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c...    26   3.6  
SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces pombe...    25   6.3  
SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase I|Schizosacchar...    25   8.4  

>SPAC31A2.02 |trm112||tRNA |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 126

 Score = 39.5 bits (88), Expect = 3e-04
 Identities = 16/65 (24%), Positives = 36/65 (55%)
 Frame = +2

Query: 263 YPLAINATDIKVKEVDFNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSLENKYDENEE 442
           +PL +    + +++++  PEF+  ++P++DW  L      +G+   LP    +  D+++E
Sbjct: 23  FPLDVVDAKLAIQQLELKPEFLIGIMPRIDWNALLKTTRQLGNY-SLPDEKPDLVDDSDE 81

Query: 443 FLKKA 457
            L K+
Sbjct: 82  VLLKS 86


>SPBC1604.17c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 459

 Score = 30.7 bits (66), Expect = 0.13
 Identities = 12/35 (34%), Positives = 21/35 (60%)
 Frame = +2

Query: 311 FNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSL 415
           F  +FI  V+ +++WE+L +     G  +GL R+L
Sbjct: 100 FQEQFIENVVKEINWEILAILISLSGIENGLVRNL 134


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 29.9 bits (64), Expect = 0.22
 Identities = 25/70 (35%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
 Frame = -2

Query: 492 PSSTSTSNKTL*AFFKNSSFSSYLFSKDLGRPSLWPMLSAATHNTSQS-SFGITRLINSG 316
           PS +STS+ T  +    S+ SSYL S  +   S  P  S+++  TS S S       +S 
Sbjct: 187 PSVSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSS 246

Query: 315 LKSTSLTLMS 286
             STS +L S
Sbjct: 247 SSSTSSSLSS 256



 Score = 25.0 bits (52), Expect = 6.3
 Identities = 19/51 (37%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
 Frame = -2

Query: 489 SSTSTSNKTL*AFFKNSSFSSYLFSKDLGRPSLW---PMLSAATHNTSQSS 346
           S TSTS+    +   +SSFSS L S  +   S +   P  S++T ++S SS
Sbjct: 299 SPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSS 349


>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1111

 Score = 25.8 bits (54), Expect = 3.6
 Identities = 12/32 (37%), Positives = 15/32 (46%)
 Frame = +2

Query: 311 FNPEFISRVIPKLDWEVLWVAADSIGHSDGLP 406
           F+PE  +  +  LDW   W  A S G    LP
Sbjct: 577 FSPEVRNGFLKTLDWLSQWACARSYGLGTRLP 608


>SPAC7D4.03c |||conserved fungal family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 886

 Score = 25.0 bits (52), Expect = 6.3
 Identities = 13/48 (27%), Positives = 22/48 (45%)
 Frame = -3

Query: 473 LTRLYELSSKTLRFHHTYFLKILADHHCGRCYQRPPIILPNPALVLHD 330
           L RL    S + R  +T  +  +A HHC    +   ++     L+L+D
Sbjct: 209 LRRLQSNISTSERITYTKAILAIAKHHCWNKVEHSALLYLEHQLILYD 256


>SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase
           I|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 859

 Score = 24.6 bits (51), Expect = 8.4
 Identities = 9/29 (31%), Positives = 14/29 (48%)
 Frame = -1

Query: 121 DYWHFFVEFS*NIFVIMYFTCIIRYLRLC 35
           D WH F  F   IF+ + +T ++     C
Sbjct: 685 DPWHMFTSFVQYIFLSIMYTNVLNVYAFC 713


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,993,843
Number of Sequences: 5004
Number of extensions: 39627
Number of successful extensions: 96
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 96
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 196153982
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)