Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30862.Seq
         (598 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC825.05c |||splicing coactivator SRRM1 |Schizosaccharomyces p...    85   1e-17
SPBC2G5.06c |hmt2|cad1|sulfide-quinone oxidoreductase|Schizosacc...    28   1.2  
SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p...    26   3.6  
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    26   4.8  
SPCC1919.13c |||conserved eukaryotic protein|Schizosaccharomyces...    25   6.3  
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc...    25   8.4  

>SPCC825.05c |||splicing coactivator SRRM1 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 301

 Score = 84.6 bits (200), Expect = 1e-17
 Identities = 48/126 (38%), Positives = 68/126 (53%), Gaps = 12/126 (9%)
 Frame = +3

Query: 255 FSDKEKKLMKQMKFGDCLTQQVDMSKVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLE 434
           F+  +KKLM+  KF      +VDM KV ++VLKPWI  ++ E++  ED+VVI +V   LE
Sbjct: 16  FTTADKKLMRSTKFPASYDTKVDMKKVNIEVLKPWIATRLNELIGFEDEVVINFVYGMLE 75

Query: 435 EKFPCPKKMADQLDWI--PKWKE----------RTPFMGELWELLLSAQASENGIPESFT 578
           E     K    Q +    P+  +           T F  ELW L++SA  ++ GIPE F 
Sbjct: 76  EAVEASKTSDSQNESTLDPRKVQLNLTGFLESNATAFTEELWSLIISASQNQYGIPEKFI 135

Query: 579 QQKKEE 596
            +KKEE
Sbjct: 136 LEKKEE 141


>SPBC2G5.06c |hmt2|cad1|sulfide-quinone
           oxidoreductase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 459

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 11/21 (52%), Positives = 13/21 (61%)
 Frame = +1

Query: 469 NLTGFLNGKNARLSWESYGSC 531
           NL  F+NGKN   S+  Y SC
Sbjct: 363 NLWSFVNGKNLTASYNGYTSC 383


>SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 505

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 13/36 (36%), Positives = 22/36 (61%)
 Frame = -1

Query: 535 SNSSHNSPMKGVRSFHLGIQSS*SAIFFGHGNFSSS 428
           +  S + P+  +RSF  G++SS + I F H  FS++
Sbjct: 379 NQQSRDDPIALLRSFLKGLESSGTGISFTHVIFSTN 414


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 25.8 bits (54), Expect = 4.8
 Identities = 20/80 (25%), Positives = 35/80 (43%), Gaps = 5/80 (6%)
 Frame = +3

Query: 258 SDKEKKLMKQMKFGDCLTQQVDMSKVKLDVLKPWITQ----KI-TEILNMEDDVVIEYVT 422
           ++K+KK  K  K     ++   +SK     L+ W  +    K+  E L   DD +  + T
Sbjct: 620 AEKDKKDKKDKKEDSKRSRSHSVSKPDSSKLRTWTDRTGAFKVEAEFLGYSDDKIHLHKT 679

Query: 423 NQLEEKFPCPKKMADQLDWI 482
           N ++   P  K     LD++
Sbjct: 680 NGVKISVPSAKMSYKDLDYV 699


>SPCC1919.13c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 282

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 19/87 (21%), Positives = 34/87 (39%)
 Frame = +3

Query: 261 DKEKKLMKQMKFGDCLTQQVDMSKVKLDVLKPWITQKITEILNMEDDVVIEYVTNQLEEK 440
           D +K L+   K    L  +  +  + L   KP+    +  +   +D      +T + +  
Sbjct: 160 DNQKMLLAFFKASKFLLSEKGVIVITLAETKPYTLWNLKGLA--KDAGYTSLMTEKFDSS 217

Query: 441 FPCPKKMADQLDWIPKWKERTPFMGEL 521
           F         + WI    ER+P+ GEL
Sbjct: 218 FYPEYSHRRTIGWIDGISERSPWKGEL 244


>SPAC27E2.06c |||methionine-tRNA ligase,
           mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 539

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = -2

Query: 573 MIRVYRFHWPARSATAPITLP*KACVLSI*ESSQVDLPFSLG 448
           +IR +  +WPA    A + LP K  V S    ++V +  SLG
Sbjct: 291 IIRFHCIYWPAFLMAAGLPLPEKILVHSHWTMNKVKMSKSLG 332


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,497,578
Number of Sequences: 5004
Number of extensions: 52878
Number of successful extensions: 137
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)