BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30854.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 32 0.073 SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe... 27 2.1 SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Sch... 27 2.1 SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 27 2.1 SPBC651.12c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 2.7 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 2.7 SPBC28F2.09 |||transcription factor TFIIA complex large subunit ... 26 3.6 SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|c... 26 4.8 SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 25 8.4 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 31.9 bits (69), Expect = 0.073 Identities = 17/39 (43%), Positives = 23/39 (58%) Frame = +1 Query: 103 NKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVAN 219 N D KFP K+P F DG L+E+ AIA+Y+A+ Sbjct: 38 NFPADLAAKFPLQKMPVFVGKDG-FPLSETLAIAFYLAS 75 Score = 30.3 bits (65), Expect = 0.22 Identities = 17/66 (25%), Positives = 36/66 (54%) Frame = +3 Query: 252 SRVWQWASWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL 431 ++V Q+ S+++SEL A + P + +++Q + A++ + D L ++T+L Sbjct: 91 AKVLQYCSFTNSELPGAFRPIIAPRVFGAPYDEQAAKEAETAIALIFARFDEELASKTYL 150 Query: 432 VXERIT 449 V R+T Sbjct: 151 VGSRLT 156 >SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 568 Score = 27.1 bits (57), Expect = 2.1 Identities = 24/65 (36%), Positives = 32/65 (49%), Gaps = 1/65 (1%) Frame = +1 Query: 97 ETNKSEDFLKKFPAGKVPAFESADGKVLLTESNAIAYYVANESLRG-GDLATQAVSGSGH 273 ET K + K AG+ P ADGK +L S I Y+ E+L G TQA++ S Sbjct: 134 ETGKRFEIQVK-GAGRTPYSRFADGKAVLRSS--IREYLCCEALYALGIPTTQALAISNL 190 Query: 274 HGLTA 288 G+ A Sbjct: 191 EGVVA 195 >SPAPB1E7.06c |eme1||Holliday junction resolvase subunit Eme1|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/62 (25%), Positives = 30/62 (48%) Frame = +1 Query: 10 PENFRAYKALIAAQYSGTDVKVAPNFVFGETNKSEDFLKKFPAGKVPAFESADGKVLLTE 189 P F++ KA + QY+ +FG +K ++F K+ ++ F S + +L+ Sbjct: 553 PNYFKSLKAELNRQYAAAVNSGTRPLLFGSLSKYQNFTKEKLESEIVRF-SFEHSILINT 611 Query: 190 SN 195 SN Sbjct: 612 SN 613 >SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 220 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/34 (47%), Positives = 18/34 (52%) Frame = +1 Query: 109 SEDFLKKFPAGKVPAFESADGKVLLTESNAIAYY 210 S D KFP K+P F ADG L+E AI Y Sbjct: 40 SADLAAKFPLQKLPVFIGADG-FELSEVIAIVKY 72 >SPBC651.12c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 273 Score = 26.6 bits (56), Expect = 2.7 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = -1 Query: 547 PNAETFISDETDAGCSHVLKSMQQCTEDNDHRQV 446 P+ +F+S + S KS+ QCT+D + R + Sbjct: 162 PSKPSFLSSSVSSPASSKQKSLFQCTKDLEKRYI 195 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 26.6 bits (56), Expect = 2.7 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +1 Query: 376 TYWPP*KYWTDIFSHAPSLLXRESLVD 456 TY PP Y D+FS APS+ + + Sbjct: 257 TYLPPFNYDHDVFSFAPSVASADQFTE 283 >SPBC28F2.09 |||transcription factor TFIIA complex large subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 369 Score = 26.2 bits (55), Expect = 3.6 Identities = 12/25 (48%), Positives = 14/25 (56%) Frame = -3 Query: 179 STFPSALSNAGTFPAGNFFKKSSDL 105 +TFP A + GTFP G F S L Sbjct: 52 ATFPWAQAPVGTFPIGQLFDPVSGL 76 >SPAC15F9.02 |seh1||nucleoporin Seh1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 25.8 bits (54), Expect = 4.8 Identities = 14/39 (35%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Frame = +1 Query: 268 GHHGLTANYCLLPALGSSLTLV--SCNSTN-RMLNVQSL 375 GH L + C P++GSS L+ +C N R+ V++L Sbjct: 213 GHTDLIRDICWAPSMGSSYYLIATACKDGNVRIFKVETL 251 >SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1174 Score = 25.0 bits (52), Expect = 8.4 Identities = 16/42 (38%), Positives = 22/42 (52%) Frame = +1 Query: 328 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSHAPSLLXRESLV 453 LV C + N ML +L Y P KY I ++ S + R SL+ Sbjct: 960 LVRCIAFNLMLQAGALKYTPLIKYIFYILTNDLSPVVRRSLL 1001 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,554,772 Number of Sequences: 5004 Number of extensions: 53031 Number of successful extensions: 163 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 163 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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