Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30850.Seq
(382 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol p... 31 0.26
At5g47180.2 68418.m05818 vesicle-associated membrane family prot... 29 0.79
At5g47180.1 68418.m05817 vesicle-associated membrane family prot... 29 0.79
At3g12010.1 68416.m01488 expressed protein contains Prosite PS00... 29 1.0
At5g43120.1 68418.m05264 tetratricopeptide repeat (TPR)-containi... 29 1.4
At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative simila... 28 1.8
At5g53920.1 68418.m06709 ribosomal protein L11 methyltransferase... 27 5.6
At5g43790.1 68418.m05355 pentatricopeptide (PPR) repeat-containi... 27 5.6
At5g24670.1 68418.m02916 cytidine/deoxycytidylate deaminase fami... 27 5.6
At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /... 27 5.6
At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p... 27 5.6
At1g17590.3 68414.m02169 CCAAT-binding transcription factor (CBF... 27 5.6
At1g17590.2 68414.m02168 CCAAT-binding transcription factor (CBF... 27 5.6
At1g17590.1 68414.m02167 CCAAT-binding transcription factor (CBF... 27 5.6
At1g13750.1 68414.m01614 calcineurin-like phosphoesterase family... 27 5.6
At5g14620.1 68418.m01714 cytosine methyltransferase (DRM2) ident... 26 9.7
At4g25120.1 68417.m03614 UvrD/REP helicase family protein contai... 26 9.7
At3g48470.1 68416.m05291 expressed protein 26 9.7
At3g09600.1 68416.m01140 myb family transcription factor contain... 26 9.7
At1g76990.3 68414.m08966 ACT domain containing protein low simil... 26 9.7
At1g76990.2 68414.m08965 ACT domain containing protein low simil... 26 9.7
At1g76990.1 68414.m08964 ACT domain containing protein low simil... 26 9.7
>At3g19390.1 68416.m02459 cysteine proteinase, putative / thiol
protease, putative contains similarity to cysteine
proteinase RD21A (thiol protease) GI:435619, SP:P43297
from [Arabidopsis thaliana]
Length = 452
Score = 31.1 bits (67), Expect = 0.26
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +1
Query: 259 KHGGECFSKGYCSQSLIYEEASDCPEGNDCC 351
++ G+C+S G C YE A+ C +G+ CC
Sbjct: 380 EYNGKCYSWGCCP----YESATCCDDGSSCC 406
>At5g47180.2 68418.m05818 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 29.5 bits (63), Expect = 0.79
Identities = 22/91 (24%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
Frame = -1
Query: 340 FLRDSLKLLHISKIDCSSLSRNILRRASESGLFYGHSVTAFDSVFLLLRTESSSGSDIRW 161
F +DS K L K+ S ++ + +R+SESG G ++ +++ + R + + ++
Sbjct: 111 FTKDSGKTLTECKLKVSYITPSTTQRSSESGATNGDGQSS-ETISTIQRLKEERDAAVKQ 169
Query: 160 T-VLSHSTQTTFLRHGSSCSMESISQKSSSL 71
T L H +T R S +S K +++
Sbjct: 170 TQQLQHELETVRRRRNQRNSGNGLSLKLAAM 200
>At5g47180.1 68418.m05817 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 29.5 bits (63), Expect = 0.79
Identities = 22/91 (24%), Positives = 44/91 (48%), Gaps = 1/91 (1%)
Frame = -1
Query: 340 FLRDSLKLLHISKIDCSSLSRNILRRASESGLFYGHSVTAFDSVFLLLRTESSSGSDIRW 161
F +DS K L K+ S ++ + +R+SESG G ++ +++ + R + + ++
Sbjct: 111 FTKDSGKTLTECKLKVSYITPSTTQRSSESGATNGDGQSS-ETISTIQRLKEERDAAVKQ 169
Query: 160 T-VLSHSTQTTFLRHGSSCSMESISQKSSSL 71
T L H +T R S +S K +++
Sbjct: 170 TQQLQHELETVRRRRNQRNSGNGLSLKLAAM 200
>At3g12010.1 68416.m01488 expressed protein contains Prosite
PS00626: Regulator of chromosome condensation (RCC1)
signature 2
Length = 685
Score = 29.1 bits (62), Expect = 1.0
Identities = 18/73 (24%), Positives = 34/73 (46%), Gaps = 3/73 (4%)
Frame = -1
Query: 238 GHSVTAFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFLRHGSSCSMESISQKSSS---LF 68
G + A DS+ LR + +++ W + +H T+ L G C + Q S++
Sbjct: 141 GMDLFACDSMLNSLRLVETKKANVNWYIYTHETRLVLLASGLQCKTFNGFQLSTAGVVRL 200
Query: 67 P*TKMHIAQNNKN 29
P +M +A++ N
Sbjct: 201 PRFEMTMARSESN 213
>At5g43120.1 68418.m05264 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515: TPR Domain
Length = 588
Score = 28.7 bits (61), Expect = 1.4
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +3
Query: 87 CEIDSIEQEDPCRRKVVCVLWLRTVHLI-SEPELDSVLS 200
C ++E++DPC RK L LR +H+I + EL LS
Sbjct: 25 CFSCAMEEKDPCVRKAWLELSLRDMHMIRDDTELALTLS 63
>At5g20320.1 68418.m02418 DEAD/DEAH box helicase, putative similar
to CAF protein [Arabidopsis thaliana] GI:6102610;
contains Pfam profiles PF00270: DEAD/DEAH box helicase,
PF00271: Helicase conserved C-terminal domain, PF03368:
Domain of unknown function, PF00636: RNase3 domain,
PF00035: Double-stranded RNA binding motif
Length = 1676
Score = 28.3 bits (60), Expect = 1.8
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -1
Query: 187 SSSGSDIRWTVLSHSTQTTFLRHGSSCSMESISQKSSSLF 68
S G D+R H+ ++ L SS S+ S S SSSLF
Sbjct: 65 SEIGDDLRSLDFDHADVSSDLHLTSSSSVSSFSSSSSSLF 104
>At5g53920.1 68418.m06709 ribosomal protein L11
methyltransferase-related similar to ribosomal protein
L11 methyltransferase; PrmA [Escherichia coli] GI:455655
Length = 371
Score = 26.6 bits (56), Expect = 5.6
Identities = 19/48 (39%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = -1
Query: 310 ISKIDCSSLSRNILRRASESGLFYGHSVT--AFDSVFLLLRTESSSGS 173
I C +LSR+ILR + L + S T + S+F L T SSS S
Sbjct: 7 IKHFPCKNLSRHILRDSRVRPLCFFTSSTPPSSFSIFASLSTSSSSSS 54
>At5g43790.1 68418.m05355 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 460
Score = 26.6 bits (56), Expect = 5.6
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = -1
Query: 367 IVTLKYNNHFLRDSLKLLHISKIDCSSLSRNILRRASESGLF 242
I+T+ ++H S KLLH+S C S + +ILR+ +F
Sbjct: 32 IITIGLSHHTYPLS-KLLHLSSTVCLSYALSILRQIPNPSVF 72
>At5g24670.1 68418.m02916 cytidine/deoxycytidylate deaminase family
protein similar to SP|Q9URQ3 tRNA-specific adenosine
deaminase 3 (EC 3.5.4.-) (tRNA-specific adenosine-34
deaminase subunit TAD3) {Saccharomyces cerevisiae};
contains Pfam profile PF00383: Cytidine and
deoxycytidylate deaminase zinc-binding region
Length = 432
Score = 26.6 bits (56), Expect = 5.6
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 4/38 (10%)
Frame = -1
Query: 166 RWTVLSHSTQTTF----LRHGSSCSMESISQKSSSLFP 65
+W++ H T+ LRH S ++ES S + +LFP
Sbjct: 270 QWSLQPHDTENCSQWHPLRHASMVAIESSSARDRNLFP 307
>At5g05110.1 68418.m00542 cysteine protease inhibitor, putative /
cystatin, putative similar to cysteine proteinase
inhibitor [Glycine max] GI:1944342; contains Pfam
profile PF00031: Cystatin domain
Length = 232
Score = 26.6 bits (56), Expect = 5.6
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -1
Query: 178 GSDIRWTVLSHSTQTTFLRHGSSCSMESISQKSSSLFP 65
G+ W S ST ++ + +M+S+ QKS+SLFP
Sbjct: 149 GNGFDWR--SVSTNNPEVQEAAKHAMKSLQQKSNSLFP 184
>At1g61210.1 68414.m06897 WD-40 repeat family protein / katanin p80
subunit, putative contains 5 WD-40 repeats (PF00400);
similar to katanin p80 subunit (GI:3005601)
[Strongylocentrotus purpuratus]
Length = 1180
Score = 26.6 bits (56), Expect = 5.6
Identities = 15/40 (37%), Positives = 19/40 (47%)
Frame = -1
Query: 223 AFDSVFLLLRTESSSGSDIRWTVLSHSTQTTFLRHGSSCS 104
AFDS +L+ +SSG W V F H S+CS
Sbjct: 65 AFDSAEVLVLAGASSGVIKLWDVEEAKMVRAFTGHRSNCS 104
>At1g17590.3 68414.m02169 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 26.6 bits (56), Expect = 5.6
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 322 KLLHISKIDCSSLSRNILRRASESGLFYGHS 230
+L + + DCS+ SR+ + AS+S +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g17590.2 68414.m02168 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 26.6 bits (56), Expect = 5.6
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 322 KLLHISKIDCSSLSRNILRRASESGLFYGHS 230
+L + + DCS+ SR+ + AS+S +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g17590.1 68414.m02167 CCAAT-binding transcription factor
(CBF-B/NF-YA) family protein contains Pfam profile:
PF02045 CCAAT-binding transcription factor (CBF-B/NF-YA)
subunit B
Length = 328
Score = 26.6 bits (56), Expect = 5.6
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -1
Query: 322 KLLHISKIDCSSLSRNILRRASESGLFYGHS 230
+L + + DCS+ SR+ + AS+S +GHS
Sbjct: 261 QLQNSNDCDCSTTSRSDITSASDSVNLFGHS 291
>At1g13750.1 68414.m01614 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 613
Score = 26.6 bits (56), Expect = 5.6
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = +1
Query: 199 AAKRRNRMLLRSVRKXDQIRKHGGECFSKGYCSQ 300
A+ + L++ ++K D + G C++ GY SQ
Sbjct: 313 ASLNTTKQLIKDLKKTDAVFHIGDICYANGYLSQ 346
>At5g14620.1 68418.m01714 cytosine methyltransferase (DRM2)
identical to cytosine methyltransferase GI:7658293 from
[Arabidopsis thaliana]
Length = 626
Score = 25.8 bits (54), Expect = 9.7
Identities = 17/58 (29%), Positives = 28/58 (48%)
Frame = +3
Query: 54 ILVYGNSEDDFCEIDSIEQEDPCRRKVVCVLWLRTVHLISEPELDSVLSSKKTESNAV 227
++++ N +DDF EID+ Q P R + + R +L S LSS ++ V
Sbjct: 1 MVIWNNDDDDFLEIDNF-QSSP-RSSPIHAMQCRVENLAGVAVTTSSLSSPTETTDLV 56
>At4g25120.1 68417.m03614 UvrD/REP helicase family protein contains
Pfam PF00580: UvrD/REP helicase
Length = 1122
Score = 25.8 bits (54), Expect = 9.7
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 105 EQEDPCRRKVVCVLWLRTVHLISEPELDSVLSSKKTESNAVT 230
E+ED + K C ++ ISE E ++ S ++ N+VT
Sbjct: 777 EEEDAIKEKKGCNQLHSFINYISERETENFRSRRRDNENSVT 818
>At3g48470.1 68416.m05291 expressed protein
Length = 1017
Score = 25.8 bits (54), Expect = 9.7
Identities = 11/24 (45%), Positives = 15/24 (62%), Gaps = 2/24 (8%)
Frame = -2
Query: 102 WNLFHRNHLH--CFRKLKCTLHKI 37
W LFHRN +H R+ KC L ++
Sbjct: 334 WTLFHRNVIHQASVRQAKCFLWQL 357
>At3g09600.1 68416.m01140 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 298
Score = 25.8 bits (54), Expect = 9.7
Identities = 12/49 (24%), Positives = 23/49 (46%)
Frame = +3
Query: 93 IDSIEQEDPCRRKVVCVLWLRTVHLISEPELDSVLSSKKTESNAVTECP 239
++ +++ DP + V +L +S P+L+S + N TE P
Sbjct: 235 VEKLKEMDPINFETVLLLMRNLTVNLSNPDLESTRKVLLSYDNVTTELP 283
>At1g76990.3 68414.m08966 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 25.8 bits (54), Expect = 9.7
Identities = 8/23 (34%), Positives = 16/23 (69%)
Frame = -1
Query: 151 SHSTQTTFLRHGSSCSMESISQK 83
SH++Q F+RH C++++ +K
Sbjct: 302 SHASQEYFIRHKDGCTLDTEGEK 324
>At1g76990.2 68414.m08965 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 25.8 bits (54), Expect = 9.7
Identities = 8/23 (34%), Positives = 16/23 (69%)
Frame = -1
Query: 151 SHSTQTTFLRHGSSCSMESISQK 83
SH++Q F+RH C++++ +K
Sbjct: 302 SHASQEYFIRHKDGCTLDTEGEK 324
>At1g76990.1 68414.m08964 ACT domain containing protein low
similarity to uridylyltransferase SP|P56884 from
Rhizobium meliloti; contains Pfam ACT domain PF01842
Length = 453
Score = 25.8 bits (54), Expect = 9.7
Identities = 8/23 (34%), Positives = 16/23 (69%)
Frame = -1
Query: 151 SHSTQTTFLRHGSSCSMESISQK 83
SH++Q F+RH C++++ +K
Sbjct: 302 SHASQEYFIRHKDGCTLDTEGEK 324
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,524,388
Number of Sequences: 28952
Number of extensions: 173085
Number of successful extensions: 552
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 540
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 552
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 527724392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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