BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30842.Seq (698 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 119 3e-28 SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 106 3e-24 SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 70 3e-13 SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 58 9e-10 SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 38 0.001 SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 35 0.010 SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 35 0.010 SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 34 0.022 SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 29 0.85 SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 28 1.1 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 27 2.6 SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 27 3.4 SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 4.5 SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 26 6.0 SPAC17G6.09 |sec62||ER protein translocation subcomplex subunit ... 25 7.9 SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 25 7.9 >SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces pombe|chr 2|||Manual Length = 180 Score = 119 bits (287), Expect = 3e-28 Identities = 54/88 (61%), Positives = 67/88 (76%) Frame = +2 Query: 254 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 433 YKL++GE+VTTIPTIGFNVE V Y+N+ F VWD+GGQ IRP WR Y+ NT II+VVDS Sbjct: 35 YKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYFQNTQGIIFVVDS 94 Query: 434 ADRDRIGISKDELVHMLREEELANAYSL 517 DR+RI + +EL ML E+EL +A L Sbjct: 95 NDRERISEAHEELQRMLNEDELRDALLL 122 Score = 43.6 bits (98), Expect = 3e-05 Identities = 20/30 (66%), Positives = 23/30 (76%) Frame = +3 Query: 177 FRGLLGAREMRILILGLDGAGKTTILINCK 266 F+ L G REMRIL++GLD AGKTTIL K Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLK 38 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/31 (41%), Positives = 20/31 (64%) Frame = +1 Query: 496 ISERVLVVLANKQDMAGCLTVAEGTPRALGL 588 + + +L+V ANKQD+ + AE T + LGL Sbjct: 116 LRDALLLVFANKQDLPNAMNAAEITDK-LGL 145 >SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 106 bits (254), Expect = 3e-24 Identities = 46/88 (52%), Positives = 63/88 (71%) Frame = +2 Query: 254 YKLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDS 433 YKL++ + V TIPT+GFNVE VTYKN+KF VWD+GGQ IRP WR Y+ T +I+VVDS Sbjct: 39 YKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDS 98 Query: 434 ADRDRIGISKDELVHMLREEELANAYSL 517 AD +RI ++ EL ++ + E+ + L Sbjct: 99 ADSNRISEARQELHRIISDREMRDCLLL 126 Score = 39.5 bits (88), Expect = 5e-04 Identities = 20/35 (57%), Positives = 22/35 (62%) Frame = +3 Query: 162 GLFSYFRGLLGAREMRILILGLDGAGKTTILINCK 266 G F L +EMRIL+LGLD AGKTTIL K Sbjct: 8 GFSKPFSRLFSNKEMRILMLGLDAAGKTTILYKLK 42 Score = 29.5 bits (63), Expect = 0.48 Identities = 13/30 (43%), Positives = 21/30 (70%) Frame = +1 Query: 481 VKGRRISERVLVVLANKQDMAGCLTVAEGT 570 + R + + +L+VLANKQD+ G L+ A+ T Sbjct: 115 ISDREMRDCLLLVLANKQDLPGALSPAQIT 144 >SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosaccharomyces pombe|chr 1|||Manual Length = 186 Score = 70.1 bits (164), Expect = 3e-13 Identities = 29/70 (41%), Positives = 48/70 (68%) Frame = +2 Query: 290 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 469 PT GF + + + L+F +WD+GGQ ++R +W+ Y+ +T+AII+VVDS D R+ ++ Sbjct: 46 PTFGFQIRTLEVEGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNT 105 Query: 470 LVHMLREEEL 499 L +L EE+L Sbjct: 106 LQELLVEEKL 115 Score = 35.9 bits (79), Expect = 0.006 Identities = 16/22 (72%), Positives = 19/22 (86%) Frame = +3 Query: 189 LGAREMRILILGLDGAGKTTIL 254 L RE+R+L+LGLD AGKTTIL Sbjct: 12 LKEREVRVLLLGLDNAGKTTIL 33 >SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharomyces pombe|chr 2|||Manual Length = 190 Score = 58.4 bits (135), Expect = 9e-10 Identities = 28/71 (39%), Positives = 41/71 (57%) Frame = +2 Query: 290 PTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDE 469 PT+ E++ N++F +DLGG R WR Y+ + I+Y+VD D +R+ SK E Sbjct: 50 PTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAE 109 Query: 470 LVHMLREEELA 502 L +L EELA Sbjct: 110 LDALLAMEELA 120 Score = 28.7 bits (61), Expect = 0.85 Identities = 13/26 (50%), Positives = 18/26 (69%) Frame = +3 Query: 198 REMRILILGLDGAGKTTILINCKSVR 275 + ++L LGLD AGKTT+L K+ R Sbjct: 19 KHAKMLFLGLDNAGKTTLLHMLKNDR 44 >SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 37.9 bits (84), Expect = 0.001 Identities = 21/57 (36%), Positives = 27/57 (47%), Gaps = 4/57 (7%) Frame = +2 Query: 287 IPTIGFNVEQVTY----KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRD 445 I TIG + + T+ K +K Q+WD GQ R YY II V D D+D Sbjct: 38 ISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQD 94 >SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 35.1 bits (77), Expect = 0.010 Identities = 16/52 (30%), Positives = 30/52 (57%) Frame = +2 Query: 293 TIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDR 448 T+G + T +L+ +++D+GGQ + R W + N ++II+ V D D+ Sbjct: 179 TLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYDK 230 >SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 35.1 bits (77), Expect = 0.010 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = +2 Query: 245 DNSYKLQVG-EVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIY 421 D+ VG + + I ++G +Q K +K Q+WD GQ R R YY + Sbjct: 35 DDQVSHTVGIDFASRIISVGMGNQQ---KRIKLQIWDTAGQEKFRSVARNYYRGAAGAVL 91 Query: 422 VVDSADRD 445 V D ++D Sbjct: 92 VYDVTNKD 99 >SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 33.9 bits (74), Expect = 0.022 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Frame = +2 Query: 278 VTTIPTIGFNVEQVTY--KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADR 442 +TTI I F + + K +K Q+WD GQ R YY I+ + D D+ Sbjct: 39 ITTIG-IDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLLYDVTDK 94 >SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 211 Score = 28.7 bits (61), Expect = 0.85 Identities = 13/54 (24%), Positives = 24/54 (44%) Frame = +2 Query: 329 NLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKDELVHMLRE 490 ++K ++WD GQ + YY N + I V D + +K + + R+ Sbjct: 63 SVKLEIWDTAGQERYKSLAPMYYRNANCAIVVYDITQAASLEKAKSWIKELQRQ 116 >SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1|||Manual Length = 214 Score = 28.3 bits (60), Expect = 1.1 Identities = 12/35 (34%), Positives = 15/35 (42%) Frame = +2 Query: 326 KNLKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVD 430 K +K Q+WD GQ R YY + V D Sbjct: 57 KKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYD 91 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/31 (32%), Positives = 19/31 (61%) Frame = -2 Query: 463 FRYSYSIPVS*VYNIYYCICVAIVTSPIRPN 371 F ++Y++ S VYN + +C + T+P + N Sbjct: 494 FDFTYNLSESEVYNTVFNLCDELYTTPCKKN 524 >SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr 1|||Manual Length = 198 Score = 26.6 bits (56), Expect = 3.4 Identities = 13/59 (22%), Positives = 28/59 (47%), Gaps = 2/59 (3%) Frame = +2 Query: 296 IGFNVEQVTYKN--LKFQVWDLGGQTSIRPYWRCYYGNTDAIIYVVDSADRDRIGISKD 466 + F + ++ +N + F +WDLGGQ + AI+++ D + + + K+ Sbjct: 45 VNFMEKTISIRNTEITFSIWDLGGQREFVNMLPMVCNDAVAILFMFDLSRKSTLNSIKE 103 >SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 407 Score = 26.2 bits (55), Expect = 4.5 Identities = 9/21 (42%), Positives = 16/21 (76%) Frame = +3 Query: 192 GAREMRILILGLDGAGKTTIL 254 G ++++L+LG +GKTTI+ Sbjct: 71 GGNDIKVLLLGAGDSGKTTIM 91 >SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9|Schizosaccharomyces pombe|chr 1|||Manual Length = 1223 Score = 25.8 bits (54), Expect = 6.0 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = -2 Query: 295 RRYSGHDLTDLQFIRIVVFPAPSNPSIKIRISLA 194 R S H+L+ + FIR + P P++ KI LA Sbjct: 1107 RNNSSHELSLVAFIREMAMPVPNDEFKKISTILA 1140 >SPAC17G6.09 |sec62||ER protein translocation subcomplex subunit Sec62 |Schizosaccharomyces pombe|chr 1|||Manual Length = 273 Score = 25.4 bits (53), Expect = 7.9 Identities = 8/16 (50%), Positives = 12/16 (75%) Frame = -2 Query: 583 PGPWVYPRLLSDIRPC 536 PG W++P LL+D+ C Sbjct: 195 PGIWLFPNLLADVGFC 210 >SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 690 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/44 (29%), Positives = 20/44 (45%) Frame = -3 Query: 135 NLLLLIRNVYALRLHIKDF*NCFFVLIYTWSTLNILFNNKYCTF 4 N + +R+ + R HI C ++ WS + L NN Y F Sbjct: 422 NKCIQLRDYFDYRKHI-----CIVTDLFGWSVFDFLKNNNYIPF 460 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,865,565 Number of Sequences: 5004 Number of extensions: 61292 Number of successful extensions: 188 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 188 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 323158234 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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