Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30833.Seq
(698 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 67 3e-12
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 51 2e-07
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 49 7e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 44 3e-05
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 42 6e-05
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 38 0.001
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 38 0.001
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 30 0.28
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 29 0.85
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.0
SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces po... 27 3.4
SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.4
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 66.9 bits (156), Expect = 3e-12
Identities = 40/120 (33%), Positives = 61/120 (50%), Gaps = 2/120 (1%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSXI-DYSGGRQADDIISWLKKKTGPPAV 435
I L +VD T+E DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P
Sbjct: 73 ISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQLLPTVK 132
Query: 436 EVTSG*TG*RTYRCQYCYCIWFLFDQSSARAKTFLSTAQVV-DDQVFAIVSDEKVIKEFG 612
++ + + F DQ T+ A+V+ DD VFA D+++ K G
Sbjct: 133 PISKDTLENFVEKADDLAVVAFFKDQK--LNDTYTEVAEVMKDDFVFAASDDKELAKSLG 190
Score = 62.1 bits (144), Expect = 7e-11
Identities = 25/53 (47%), Positives = 40/53 (75%), Gaps = 1/53 (1%)
Frame = +2
Query: 95 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAEE 250
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + +++
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDD 405
Score = 58.8 bits (136), Expect = 7e-10
Identities = 22/45 (48%), Positives = 33/45 (73%)
Frame = +2
Query: 116 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEE 250
++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L ++
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADELEKD 71
Score = 34.7 bits (76), Expect = 0.013
Identities = 18/55 (32%), Positives = 34/55 (61%), Gaps = 3/55 (5%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSXID---YSGGRQADDIISWLKK 414
+ +AK+DAT E D++ S + G+PT+ FF+ ++ Y G R +D+ +++ K
Sbjct: 408 VVVAKIDAT-ENDISVS--ISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 50.8 bits (116), Expect = 2e-07
Identities = 24/67 (35%), Positives = 31/67 (46%)
Frame = +2
Query: 41 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 220
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 221 AKAATKL 241
K A+ L
Sbjct: 71 QKLASNL 77
Score = 29.5 bits (63), Expect = 0.48
Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 6/49 (12%)
Frame = +1
Query: 253 IPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSXI---DYSGGR 381
+P+ DA Q + + Y V+G+PT+K GS + DY+G R
Sbjct: 81 LPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 48.8 bits (111), Expect = 7e-07
Identities = 21/40 (52%), Positives = 27/40 (67%), Gaps = 1/40 (2%)
Frame = +2
Query: 104 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEY 220
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY 180
Score = 46.4 bits (105), Expect = 4e-06
Identities = 24/65 (36%), Positives = 34/65 (52%)
Frame = +2
Query: 26 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 205
+ + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56
Query: 206 LAPEY 220
LAP Y
Sbjct: 57 LAPVY 61
Score = 44.0 bits (99), Expect = 2e-05
Identities = 20/57 (35%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-XIDYSGGRQADDIISWLKKKTG 423
+ + K+DA D+A+ Y + G+PTL +F +GS + YS R D + ++ +KTG
Sbjct: 74 VLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLTQFVSEKTG 130
Score = 35.1 bits (77), Expect = 0.010
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSXID---YSGGRQADDIISWLKKKTG 423
+++ K++A D+ + V +PT+KFF Y G R + +I ++ KK+G
Sbjct: 193 VEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 43.6 bits (98), Expect = 3e-05
Identities = 13/49 (26%), Positives = 32/49 (65%)
Frame = +2
Query: 113 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEEFL 259
V + F++++ + ++V+F+A WCG CK++AP++ + + ++ F+
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI 53
Score = 27.1 bits (57), Expect = 2.6
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +1
Query: 271 KVDATQEQDLAESYGVRGYPTLKFFRNGSXID 366
KVD Q ++A GV P+ ++NG I+
Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 42.3 bits (95), Expect = 6e-05
Identities = 16/49 (32%), Positives = 32/49 (65%)
Frame = +2
Query: 113 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEEFL 259
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A +F+
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPKFV 54
Score = 35.5 bits (78), Expect = 0.007
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +1
Query: 268 AKVDATQEQDLAESYGVRGYPTLKFFRNGSXIDYSGG 378
AKV+ +++ +A GV+ PT FF NG ID G
Sbjct: 56 AKVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTG 92
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 38.3 bits (85), Expect = 0.001
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +2
Query: 113 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEEFLS 262
V S ++ T I+ + +V+FYA WCG CK L P K + + + F++
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFIA 71
Score = 31.1 bits (67), Expect = 0.16
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 274 VDATQEQDLAESYGVRGYPTLKFFRNGSXIDYSGGRQADDIISWLKK 414
V+A + D+A+ GV PT+ FR G +D G + S L K
Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 37.9 bits (84), Expect = 0.001
Identities = 13/64 (20%), Positives = 36/64 (56%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSXIDYSGGRQADDIISWLKKKTGPPAVE 438
+K+A+V+ +E+++ + ++ +PT + F+ I Y+G + ++S+ + A++
Sbjct: 230 LKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNGPLKYQQLLSYSNQVASYQAIK 289
Query: 439 VTSG 450
+ G
Sbjct: 290 IEEG 293
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 30.3 bits (65), Expect = 0.28
Identities = 10/52 (19%), Positives = 28/52 (53%)
Frame = +1
Query: 259 IKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSXIDYSGGRQADDIISWLKK 414
+ +A ++ + + Y ++ +PT FF+ + ++Y G D++S+ ++
Sbjct: 331 LNVAHINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEE 382
Score = 28.7 bits (61), Expect = 0.85
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 167 VEFYAPWCGHCKSLAPEYAKAATKLAEEEFLSN 265
+++Y P CG CK L P + K E+ SN
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSN 79
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 28.7 bits (61), Expect = 0.85
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +2
Query: 155 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEEFL 259
+ IL+ FYAPW CK + + + A FL
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL 55
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.5 bits (58), Expect = 2.0
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +3
Query: 102 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 224
K +F L LQP T N +L+ + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660
>SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 742
Score = 26.6 bits (56), Expect = 3.4
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -3
Query: 489 ITVLASISSLACSARGDLNSRXASLLLQPTDDVISLTTT*IVDXT 355
+T+ S+ ++G+L+ SLL +D+V +TTT +VD T
Sbjct: 295 LTICPSLQGFEFDSKGNLD---VSLLKSLSDEVNMITTTSLVDNT 336
>SPBC4B4.11 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 113
Score = 26.6 bits (56), Expect = 3.4
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = -2
Query: 568 LGHQQLEQLKGKFWLWLSSGRKETKYNNSIGID 470
+G E++ GKF W RK TK GID
Sbjct: 43 IGRDTAEKVNGKFESWDGLFRKSTKTMKKEGID 75
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,680,312
Number of Sequences: 5004
Number of extensions: 50254
Number of successful extensions: 160
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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