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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30823.Seq
         (458 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B) ribo...    88   2e-18
At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)            86   1e-17
At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A)            83   9e-17
At3g19400.2 68416.m02460 cysteine proteinase, putative non-conse...    32   0.21 
At3g19400.1 68416.m02461 cysteine proteinase, putative non-conse...    32   0.21 
At4g17250.1 68417.m02594 expressed protein                             29   2.0  
At3g43960.1 68416.m04706 cysteine proteinase, putative contains ...    27   6.1  
At3g13860.1 68416.m01751 chaperonin, putative similar to SWISS-P...    27   6.1  
At5g37080.1 68418.m04451 hypothetical protein includes At5g37080...    27   8.1  

>At4g26230.1 68417.m03776 60S ribosomal protein L31 (RPL31B)
           ribosomal protein L31, Nicotiana glutinosa, U23784
          Length = 119

 Score = 88.2 bits (209), Expect = 2e-18
 Identities = 38/50 (76%), Positives = 45/50 (90%)
 Frame = +1

Query: 103 VTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNK 252
           VTREYT+NLH+RLH   FKK+AP+AIKEIRKFAEK+MGT D+RVD +LNK
Sbjct: 12  VTREYTINLHRRLHSCTFKKKAPKAIKEIRKFAEKEMGTKDVRVDVKLNK 61



 Score = 44.4 bits (100), Expect = 4e-05
 Identities = 27/90 (30%), Positives = 40/90 (44%), Gaps = 2/90 (2%)
 Frame = +3

Query: 147 CWI*KACPKSNQRNPKVR*KTDGNSGHSSRHSLKQILWSKGVRNVPFXXXXXXXXXXNDD 326
           C   K  PK+ +   K   K  G         L + +WSKG+R  P           NDD
Sbjct: 27  CTFKKKAPKAIKEIRKFAEKEMGTKDVRVDVKLNKQIWSKGIRGPPRRIRVRVARKRNDD 86

Query: 327 EDSAHKLFTLVTY--VPVASIKGLQTENVD 410
           ED+  + F+LVT   +P   + GL T+ ++
Sbjct: 87  EDAKEEFFSLVTVAEIPAEGLSGLGTKIIE 116


>At5g56710.1 68418.m07078 60S ribosomal protein L31 (RPL31C)
          Length = 119

 Score = 85.8 bits (203), Expect = 1e-17
 Identities = 37/50 (74%), Positives = 44/50 (88%)
 Frame = +1

Query: 103 VTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNK 252
           +TREYT+NLH+RLH   FKK+AP+AIKEIRKFAEK MGT D+RVD +LNK
Sbjct: 12  ITREYTINLHRRLHKCTFKKKAPKAIKEIRKFAEKAMGTKDVRVDVKLNK 61



 Score = 44.8 bits (101), Expect = 3e-05
 Identities = 27/90 (30%), Positives = 40/90 (44%), Gaps = 2/90 (2%)
 Frame = +3

Query: 147 CWI*KACPKSNQRNPKVR*KTDGNSGHSSRHSLKQILWSKGVRNVPFXXXXXXXXXXNDD 326
           C   K  PK+ +   K   K  G         L + +WSKG+R  P           NDD
Sbjct: 27  CTFKKKAPKAIKEIRKFAEKAMGTKDVRVDVKLNKQIWSKGIRGPPRRIRVRVARKRNDD 86

Query: 327 EDSAHKLFTLVTY--VPVASIKGLQTENVD 410
           ED+  + F+LVT   +P   + GL T+ ++
Sbjct: 87  EDAKEEFFSLVTVAEIPAEGLSGLGTKVIE 116


>At2g19740.1 68415.m02306 60S ribosomal protein L31 (RPL31A)
          Length = 119

 Score = 83.0 bits (196), Expect = 9e-17
 Identities = 37/50 (74%), Positives = 42/50 (84%)
 Frame = +1

Query: 103 VTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNK 252
           VTREYT+NLH+RLH   FKK+AP AIKEIRKFA K MGT D+RVD +LNK
Sbjct: 12  VTREYTINLHRRLHSCTFKKKAPNAIKEIRKFALKAMGTKDVRVDVKLNK 61



 Score = 43.2 bits (97), Expect = 9e-05
 Identities = 26/93 (27%), Positives = 40/93 (43%), Gaps = 2/93 (2%)
 Frame = +3

Query: 147 CWI*KACPKSNQRNPKVR*KTDGNSGHSSRHSLKQILWSKGVRNVPFXXXXXXXXXXNDD 326
           C   K  P + +   K   K  G         L + +WSKG+R  P           NDD
Sbjct: 27  CTFKKKAPNAIKEIRKFALKAMGTKDVRVDVKLNKQIWSKGIRGPPRRIRVRVARKRNDD 86

Query: 327 EDSAHKLFTLVTY--VPVASIKGLQTENVDASQ 419
           ED+  + F+LVT   +P   + GL T+ ++  +
Sbjct: 87  EDAKEEFFSLVTVAEIPAEGLSGLGTKVIEEEE 119


>At3g19400.2 68416.m02460 cysteine proteinase, putative
           non-consensus AT acceptor site at exon 3; contains
           similarity to cysteine protease CYP1 GI:2828252, TDI-65
           GI:5726641 from [Lycopersicon esculentum]
          Length = 290

 Score = 31.9 bits (69), Expect = 0.21
 Identities = 17/67 (25%), Positives = 31/67 (46%)
 Frame = +1

Query: 76  KRQISHKRSVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 255
           +R  +  R +  ++ V   K  +G+G K+R  +  K+  KF ++    PD   +  L +F
Sbjct: 34  ERNETEVRLMYEQWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93

Query: 256 FGLRESE 276
             L   E
Sbjct: 94  ADLTNEE 100


>At3g19400.1 68416.m02461 cysteine proteinase, putative
           non-consensus AT acceptor site at exon 3; contains
           similarity to cysteine protease CYP1 GI:2828252, TDI-65
           GI:5726641 from [Lycopersicon esculentum]
          Length = 362

 Score = 31.9 bits (69), Expect = 0.21
 Identities = 17/67 (25%), Positives = 31/67 (46%)
 Frame = +1

Query: 76  KRQISHKRSVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKF 255
           +R  +  R +  ++ V   K  +G+G K+R  +  K+  KF ++    PD   +  L +F
Sbjct: 34  ERNETEVRLMYEQWLVENRKNYNGLGEKERRFKIFKDNLKFVDEHNSVPDRTFEVGLTRF 93

Query: 256 FGLRESE 276
             L   E
Sbjct: 94  ADLTNEE 100


>At4g17250.1 68417.m02594 expressed protein
          Length = 416

 Score = 28.7 bits (61), Expect = 2.0
 Identities = 16/47 (34%), Positives = 26/47 (55%)
 Frame = +1

Query: 118 TVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKFF 258
           TV   KR   + +KKR   A+K+   + E++ G P ++ D  L KF+
Sbjct: 169 TVPATKRFLELKYKKRYEFALKQCPSYTERRRGVPKLKED--LMKFW 213


>At3g43960.1 68416.m04706 cysteine proteinase, putative contains
           similarity to cysteine proteinase RD21A (thiol protease)
           GI:435619, SP:P43297 from [Arabidopsis thaliana]
          Length = 376

 Score = 27.1 bits (57), Expect = 6.1
 Identities = 16/55 (29%), Positives = 26/55 (47%)
 Frame = +1

Query: 112 EYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPDIRVDTRLNKFFGLRESE 276
           ++ V   K  +G+G K+R  +  K+  K  E+    P+   +  LNKF  L   E
Sbjct: 43  QWLVENGKNYNGLGEKERRFKIFKDNLKRIEEHNSDPNRSYERGLNKFSDLTADE 97


>At3g13860.1 68416.m01751 chaperonin, putative similar to
           SWISS-PROT:P29197- chaperonin CPN60, mitochondrial
           precursor (HSP60) [Arabidopsis thaliana] ; contains
           Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family
          Length = 572

 Score = 27.1 bits (57), Expect = 6.1
 Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
 Frame = +1

Query: 94  KRSVTREYTVNLHKRLHGVGFKKRAPRAIKEIRKFAEKQMGTPD-IRVDTRLNKFFG 261
           K +VTR+ T+ LH    G G KK      +E+R   EK   T D  +   RL+K  G
Sbjct: 352 KVTVTRDDTIILH----GGGDKKLIEERCEELRSANEKSTSTFDQEKTQERLSKLSG 404


>At5g37080.1 68418.m04451 hypothetical protein includes At5g37080,
           At5g37170, At2g05090
          Length = 566

 Score = 26.6 bits (56), Expect = 8.1
 Identities = 13/45 (28%), Positives = 20/45 (44%)
 Frame = +3

Query: 126 FTQTTSWCWI*KACPKSNQRNPKVR*KTDGNSGHSSRHSLKQILW 260
           F   T W W    CPK N++   V+  T  ++G   +  +K   W
Sbjct: 295 FAIDTDWGWFYFGCPKCNRKTELVKEST--STGKMVKTPMKPKFW 337


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,917,746
Number of Sequences: 28952
Number of extensions: 163162
Number of successful extensions: 326
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 313
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 326
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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