BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30793.Seq (748 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 151 8e-38 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 36 0.008 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 33 0.033 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 32 0.076 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 32 0.100 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 31 0.17 SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 30 0.30 SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 0.93 SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 0.93 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.2 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.6 SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 2.8 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 27 3.8 SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 26 5.0 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.6 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 6.6 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.6 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 8.7 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 25 8.7 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 151 bits (367), Expect = 8e-38 Identities = 77/175 (44%), Positives = 104/175 (59%) Frame = +1 Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402 + +G+ + IVL+D+ D R+ D+V+I PCP +KY +R+ +L Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137 Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582 P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F G+ K F Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVS 197 Query: 583 LIP*YTVDGEPIKREEEEEALNAVGYDDIGGCRKTTGRKLREMVELPLRHPSLFK 747 +GEPI RE+EE +L VGYDDIGGCR+ ++RE+VELPLRHP LFK Sbjct: 198 QDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQMA-QIRELVELPLRHPQLFK 251 Score = 88.6 bits (210), Expect = 8e-19 Identities = 41/68 (60%), Positives = 53/68 (77%) Frame = +2 Query: 53 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232 N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 233 GKRRKETV 256 GKRRK+TV Sbjct: 81 GKRRKDTV 88 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 35.5 bits (78), Expect = 0.008 Identities = 17/31 (54%), Positives = 21/31 (67%) Frame = +1 Query: 652 VGYDDIGGCRKTTGRKLREMVELPLRHPSLF 744 V Y D+GGC++ R LRE+VELPL P F Sbjct: 176 VTYGDVGGCKEQIER-LREVVELPLLSPERF 205 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 33.5 bits (73), Expect = 0.033 Identities = 12/30 (40%), Positives = 23/30 (76%) Frame = +1 Query: 658 YDDIGGCRKTTGRKLREMVELPLRHPSLFK 747 Y+ +GG K ++++E++ELP++HP LF+ Sbjct: 144 YEMVGGLEKQI-KEIKEVIELPVKHPELFE 172 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 32.3 bits (70), Expect = 0.076 Identities = 16/48 (33%), Positives = 26/48 (54%) Frame = +1 Query: 604 DGEPIKREEEEEALNAVGYDDIGGCRKTTGRKLREMVELPLRHPSLFK 747 D +P+ + + Y DIGG ++++E VELPL HP L++ Sbjct: 172 DTDPMINVMKLDKAPTESYADIGGLESQI-QEIKEAVELPLTHPELYE 218 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 31.9 bits (69), Expect = 0.100 Identities = 13/31 (41%), Positives = 22/31 (70%) Frame = +1 Query: 652 VGYDDIGGCRKTTGRKLREMVELPLRHPSLF 744 + + +GG + R+LRE++ELPL++P LF Sbjct: 129 ISFAGVGGLNEQI-RELREVIELPLKNPELF 158 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 31.1 bits (67), Expect = 0.17 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +1 Query: 646 NAVGYDDIGGCRKTTGRKLREMVELPLRHPSLFK 747 +AV + IGG + + +R++VELP ++P LFK Sbjct: 274 SAVTFSSIGGLQAQIAQ-IRDIVELPFQNPELFK 306 >SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 641 Score = 30.3 bits (65), Expect = 0.30 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Frame = -3 Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384 DH L +H PA+ LK I A++ G T +PV P+ ++G + TRF Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410 >SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 0.93 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -1 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 0.93 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -1 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 28.3 bits (60), Expect = 1.2 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +3 Query: 78 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 27.9 bits (59), Expect = 1.6 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -1 Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281 LV + LKD WI + + E F G ++FS+QG Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472 >SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 382 Score = 27.1 bits (57), Expect = 2.8 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +3 Query: 27 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200 F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342 Query: 201 NS 206 S Sbjct: 343 RS 344 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 26.6 bits (56), Expect = 3.8 Identities = 11/34 (32%), Positives = 22/34 (64%) Frame = +1 Query: 646 NAVGYDDIGGCRKTTGRKLREMVELPLRHPSLFK 747 +++ ++ +GG + L+EMV LPL +P +F+ Sbjct: 261 SSLSFESVGGLDNYINQ-LKEMVMLPLLYPEIFQ 293 >SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 26.2 bits (55), Expect = 5.0 Identities = 14/48 (29%), Positives = 21/48 (43%) Frame = -1 Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248 L FH ++ D W PH+ + G F H + +G + R GF Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +3 Query: 99 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239 SSV S S ++M++ + +F+ S+ +SSV T + S +N Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +2 Query: 77 PD-DLSTAILRRKDRPNRLIVEEAVSD 154 PD D + + + RK+ PNRL++ A+S+ Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 6.6 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.4 bits (53), Expect = 8.7 Identities = 10/38 (26%), Positives = 22/38 (57%) Frame = +2 Query: 137 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRKE 250 E+AV D+N+ ++L+ + + RGD ++ G ++ Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERD 284 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 25.4 bits (53), Expect = 8.7 Identities = 14/48 (29%), Positives = 25/48 (52%) Frame = +1 Query: 601 VDGEPIKREEEEEALNAVGYDDIGGCRKTTGRKLREMVELPLRHPSLF 744 +D + I+ + + ++ IGG +L+EMV LPL +P +F Sbjct: 352 IDEKTIRSTDPFANRENLDFNSIGGLEDII-LQLKEMVMLPLLYPEVF 398 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,113,960 Number of Sequences: 5004 Number of extensions: 62355 Number of successful extensions: 189 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 181 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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