BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30782.Seq (598 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c... 44 8e-05 At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c... 44 8e-05 At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /... 41 7e-04 At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c... 39 0.002 At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook pr... 32 0.33 At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook pr... 32 0.33 At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook pr... 32 0.33 At5g59950.3 68418.m07518 RNA and export factor-binding protein, ... 29 1.8 At5g59950.2 68418.m07519 RNA and export factor-binding protein, ... 29 1.8 At5g59950.1 68418.m07517 RNA and export factor-binding protein, ... 29 1.8 At3g45830.1 68416.m04960 expressed protein 29 1.8 At2g45590.1 68415.m05669 protein kinase family protein contains ... 29 1.8 At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138... 29 3.1 At2g41420.1 68415.m05111 proline-rich family protein contains pr... 29 3.1 At5g60980.2 68418.m07650 nuclear transport factor 2 (NTF2) famil... 28 4.1 At5g60980.1 68418.m07649 nuclear transport factor 2 (NTF2) famil... 28 4.1 At2g07740.1 68415.m01002 zinc knuckle (CCHC-type) family protein... 28 4.1 At1g25540.1 68414.m03171 phytochrome and flowering time regulato... 28 4.1 At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to ... 27 7.2 At5g45190.1 68418.m05547 cyclin family protein similar to cyclin... 27 7.2 At5g35770.1 68418.m04285 sterile apetala (SAP) identical to ster... 27 7.2 At4g19600.1 68417.m02880 cyclin family protein similar to cyclin... 27 7.2 At4g16550.1 68417.m02504 heat shock protein-related contains sim... 27 7.2 At2g47920.1 68415.m05991 kinase interacting family protein simil... 27 7.2 At2g39650.1 68415.m04862 expressed protein contains Pfam profile... 27 7.2 >At4g38680.1 68417.m05477 cold-shock DNA-binding family protein contains Pfam domains PF00313: 'Cold-shock' DNA-binding domain and PF00098: Zinc knuckle Length = 203 Score = 44.0 bits (99), Expect = 8e-05 Identities = 33/103 (32%), Positives = 49/103 (47%), Gaps = 1/103 (0%) Frame = +1 Query: 202 GHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAV-VAGEKG 378 G + Q+ F DD +D+FVHQ++I + RS+ EAVEF V + Sbjct: 13 GSVKWFDTQKGFGFITPDDGGDDLFVHQSSIR----SEGFRSLAAEEAVEFEVEIDNNNR 68 Query: 379 FEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQGGGRSGEG 507 +A V+GP G PV+G+ + G + +GGGR G Sbjct: 69 PKAIDVSGPDGAPVQGN--SGGGSSGGRGGFGGGRGGGRGSGG 109 Score = 29.9 bits (64), Expect = 1.3 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = +2 Query: 191 EKVSGTVKWFNVKSGYGFI 247 E+ G+VKWF+ + G+GFI Sbjct: 9 ERRKGSVKWFDTQKGFGFI 27 >At4g36020.1 68417.m05128 cold-shock DNA-binding family protein contains Pfam domains, PF00313: 'Cold-shock' DNA-binding domain and PF00098: Zinc knuckle Length = 299 Score = 44.0 bits (99), Expect = 8e-05 Identities = 34/88 (38%), Positives = 45/88 (51%), Gaps = 1/88 (1%) Frame = +1 Query: 241 FHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKG-FEAAGVTGPGGEP 417 F DD ++FVHQ++I + RS+ G+AVEFA+ G G +A VT PGG Sbjct: 26 FITPDDGSVELFVHQSSIVS----EGYRSLTVGDAVEFAITQGSDGKTKAVNVTAPGG-- 79 Query: 418 VKGSPYAADKRRGYHRQYFPRQGGGRSG 501 GS + RG R+GGG SG Sbjct: 80 --GSLKKENNSRGNG----ARRGGGGSG 101 Score = 30.3 bits (65), Expect = 1.0 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +2 Query: 188 AEKVSGTVKWFNVKSGYGFI 247 A + +G V WFN GYGFI Sbjct: 8 AARSTGKVNWFNASKGYGFI 27 >At2g21060.1 68415.m02500 cold-shock DNA-binding family protein / glycine-rich protein (GRP2) identical to Glycine-rich protein 2b (AtGRP2b) [Arabidopsis thaliana] SWISS-PROT:Q38896; contains Pfam domains PF00313: 'Cold-shock' DNA-binding domain and PF00098: Zinc knuckle Length = 201 Score = 40.7 bits (91), Expect = 7e-04 Identities = 34/101 (33%), Positives = 47/101 (46%), Gaps = 1/101 (0%) Frame = +1 Query: 202 GHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGF 381 G + Q+ F D +D+FVHQ++I + RS+ E+VEF V G Sbjct: 17 GTVKWFDTQKGFGFITPSDGGDDLFVHQSSIR----SEGFRSLAAEESVEFDVEVDNSGR 72 Query: 382 -EAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQGGGRSG 501 +A V+GP G PV+G+ G R F GGGR G Sbjct: 73 PKAIEVSGPDGAPVQGNSGGGGSSGG--RGGF-GGGGGRGG 110 Score = 30.3 bits (65), Expect = 1.0 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = +2 Query: 191 EKVSGTVKWFNVKSGYGFI 247 ++ GTVKWF+ + G+GFI Sbjct: 13 DRRKGTVKWFDTQKGFGFI 31 >At2g17870.1 68415.m02070 cold-shock DNA-binding family protein contains Pfam domains, PF00313: 'Cold-shock' DNA-binding domain and PF00098: Zinc knuckle Length = 301 Score = 39.1 bits (87), Expect = 0.002 Identities = 26/73 (35%), Positives = 37/73 (50%), Gaps = 1/73 (1%) Frame = +1 Query: 196 SIGHCEMVQRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEK 375 SIG + F DD E++FVHQ++I + RS+ GE+VE+ + G Sbjct: 11 SIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSD----GFRSLTLGESVEYEIALGSD 66 Query: 376 G-FEAAGVTGPGG 411 G +A VT PGG Sbjct: 67 GKTKAIEVTAPGG 79 >At2g33620.3 68415.m04122 DNA-binding family protein / AT-hook protein 1 (AHP1) identical to AT-hook protein 1 [Arabidopsis thaliana] gi|2598227|emb|CAA10857 Length = 351 Score = 31.9 bits (69), Expect = 0.33 Identities = 21/54 (38%), Positives = 26/54 (48%) Frame = +1 Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQG 486 S G+ + + GE G G+TG G EPVK KRRG R+Y P G Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGPDSG 111 >At2g33620.2 68415.m04121 DNA-binding family protein / AT-hook protein 1 (AHP1) identical to AT-hook protein 1 [Arabidopsis thaliana] gi|2598227|emb|CAA10857 Length = 351 Score = 31.9 bits (69), Expect = 0.33 Identities = 21/54 (38%), Positives = 26/54 (48%) Frame = +1 Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQG 486 S G+ + + GE G G+TG G EPVK KRRG R+Y P G Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGPDSG 111 >At2g33620.1 68415.m04120 DNA-binding family protein / AT-hook protein 1 (AHP1) identical to AT-hook protein 1 [Arabidopsis thaliana] gi|2598227|emb|CAA10857 Length = 351 Score = 31.9 bits (69), Expect = 0.33 Identities = 21/54 (38%), Positives = 26/54 (48%) Frame = +1 Query: 325 SVGDGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQG 486 S G+ + + GE G G+TG G EPVK KRRG R+Y P G Sbjct: 69 SAGENSVLNMNLPGGESG----GMTGTGSEPVK-------KRRGRPRKYGPDSG 111 >At5g59950.3 68418.m07518 RNA and export factor-binding protein, putative Length = 242 Score = 29.5 bits (63), Expect = 1.8 Identities = 17/58 (29%), Positives = 26/58 (44%) Frame = +1 Query: 334 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQGGGRSGEG 507 DG+ ++ +V AA P G+P+ + RG ++ R GGGR G G Sbjct: 152 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGG 209 >At5g59950.2 68418.m07519 RNA and export factor-binding protein, putative Length = 178 Score = 29.5 bits (63), Expect = 1.8 Identities = 17/58 (29%), Positives = 26/58 (44%) Frame = +1 Query: 334 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQGGGRSGEG 507 DG+ ++ +V AA P G+P+ + RG ++ R GGGR G G Sbjct: 88 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGG 145 >At5g59950.1 68418.m07517 RNA and export factor-binding protein, putative Length = 244 Score = 29.5 bits (63), Expect = 1.8 Identities = 17/58 (29%), Positives = 26/58 (44%) Frame = +1 Query: 334 DGEAVEFAVVAGEKGFEAAGVTGPGGEPVKGSPYAADKRRGYHRQYFPRQGGGRSGEG 507 DG+ ++ +V AA P G+P+ + RG ++ R GGGR G G Sbjct: 154 DGKPMKIEIVGTNLQTAAAPSGRPANGNSNGAPWRGGQGRGGQQRGGGRGGGGRGGGG 211 >At3g45830.1 68416.m04960 expressed protein Length = 1298 Score = 29.5 bits (63), Expect = 1.8 Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 2/81 (2%) Frame = +1 Query: 220 QRQEWIWFHQQDDTK--EDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGFEAAG 393 +R+ W++ H+ + + ED T + ++A + EAV A + E+ E Sbjct: 1161 ERKLWVYLHRDREQEDFEDDGTSSTKKWKRPKKEAAEQTEEQEAVTVAFLGNEEQTE--- 1217 Query: 394 VTGPGGEPVKGSPYAADKRRG 456 T G EP G P D +G Sbjct: 1218 -TEMGSEPKTGEPTGLDGDQG 1237 >At2g45590.1 68415.m05669 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 683 Score = 29.5 bits (63), Expect = 1.8 Identities = 22/85 (25%), Positives = 32/85 (37%) Frame = +1 Query: 238 WFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAGEKGFEAAGVTGPGGEP 417 WFH+ DD V H + R ++ DG + E V G+K + +G+ Sbjct: 472 WFHR-DDGASSVHDHNLNPTKRKKRNSIDWWVDGLSGELKSVMGKKNSQDSGLWCDVNVQ 530 Query: 418 VKGSPYAADKRRGYHRQYFPRQGGG 492 G + RG P GGG Sbjct: 531 KSGGVSSTPSMRGTVCYIAPECGGG 555 >At3g30300.1 68416.m03826 expressed protein contains Pfam PF03138: Plant protein family. The function of this family of plant proteins is unknown; previously annotated as 'auxin-independent growth promoter -related' based on similarity to axi 1 protein (GB:X80301) (GI:559920) from [Nicotiana tabacum], which, due to scienitific fraud was retracted. Retraction in: Schell J. EMBO J 1999 May 17;18(10):2908. PMID:10400497. Length = 677 Score = 28.7 bits (61), Expect = 3.1 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = -3 Query: 536 HES*SSSAWTPSPLRPPP*RGKYW 465 H+S S+ P PL PPP R KY+ Sbjct: 419 HDSLKSTRQRPQPLPPPPARPKYY 442 >At2g41420.1 68415.m05111 proline-rich family protein contains proline rich extensin domains, INTERPRO:IPR002965 Length = 98 Score = 28.7 bits (61), Expect = 3.1 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = +1 Query: 391 GVTGPGGEPVKGSPYAADKRRGYHRQYFPRQG 486 GV P G P +G P A +GY Q +P+QG Sbjct: 10 GVPPPQGYPPEGYPKDAYPPQGYPPQGYPQQG 41 >At5g60980.2 68418.m07650 nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein G3BP ras-GTPase-activating protein SH3-domain binding protein, Mus musculus, EMBL:MMU65313 Length = 460 Score = 28.3 bits (60), Expect = 4.1 Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 2/49 (4%) Frame = +1 Query: 367 GEKGFEAAGVTGPGGEPVKGSPYAADKRRGYH-RQYFPRQ-GGGRSGEG 507 G +G G G GG G P +++ R G Q P+ GGGR G G Sbjct: 399 GGRGGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNGGGGRGGRG 447 >At5g60980.1 68418.m07649 nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein G3BP ras-GTPase-activating protein SH3-domain binding protein, Mus musculus, EMBL:MMU65313 Length = 459 Score = 28.3 bits (60), Expect = 4.1 Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 2/49 (4%) Frame = +1 Query: 367 GEKGFEAAGVTGPGGEPVKGSPYAADKRRGYH-RQYFPRQ-GGGRSGEG 507 G +G G G GG G P +++ R G Q P+ GGGR G G Sbjct: 398 GGRGGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNGGGGRGGRG 446 >At2g07740.1 68415.m01002 zinc knuckle (CCHC-type) family protein similar to zinc finger protein GB:AAA75253; some members of this protein family have a weak CCHC zinc fingers that is mostly from retroviral gag proteins (nucleocapsid) Length = 409 Score = 28.3 bits (60), Expect = 4.1 Identities = 15/39 (38%), Positives = 23/39 (58%), Gaps = 3/39 (7%) Frame = +1 Query: 400 GPGGEPVKGSP---YAADKRRGYHRQYFPRQGGGRSGEG 507 GP G+ V+G D+R G+++ P +GGGR G+G Sbjct: 280 GPDGKLVEGKGRLGVITDRRAGHYK---PMKGGGRKGKG 315 >At1g25540.1 68414.m03171 phytochrome and flowering time regulatory protein (PFT1) PMID: 12815435 Length = 836 Score = 28.3 bits (60), Expect = 4.1 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = +2 Query: 218 FNVKSGYGFINRMTPRKMCLCIRLPS 295 F S +GF+ ++ +K+C I+LPS Sbjct: 623 FRAMSQHGFLGQLQDKKLCAVIQLPS 648 >At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to MtN3 GI:1619602 (root nodule development) from [Medicago truncatula] Length = 294 Score = 27.5 bits (58), Expect = 7.2 Identities = 11/24 (45%), Positives = 17/24 (70%) Frame = +2 Query: 293 SPVTTHVRLCARSATERRWSLPWL 364 +PV T VR+C + +TE SLP++ Sbjct: 26 APVPTFVRICKKKSTEGFQSLPYV 49 >At5g45190.1 68418.m05547 cyclin family protein similar to cyclin T1 [Equus caballus] GI:5052355; contains Pfam profile PF00134: Cyclin, N-terminal domain Length = 579 Score = 27.5 bits (58), Expect = 7.2 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = -1 Query: 76 GERRWWQRW*NTPRPLLDV 20 GE+ WWQ + TPR L DV Sbjct: 241 GEKVWWQEFDVTPRQLEDV 259 >At5g35770.1 68418.m04285 sterile apetala (SAP) identical to sterile apetala (GI:9758652) [Arabidopsis thaliana]. Length = 446 Score = 27.5 bits (58), Expect = 7.2 Identities = 11/31 (35%), Positives = 17/31 (54%) Frame = +1 Query: 211 EMVQRQEWIWFHQQDDTKEDVFVHQTAIARN 303 +++ RQ W H DT D F+++ ARN Sbjct: 85 QLLSRQVWARTHLMHDTWRDEFIYRHRTARN 115 >At4g19600.1 68417.m02880 cyclin family protein similar to cyclin T2a [Homo sapiens] GI:2981198; contains Pfam profile PF00134: Cyclin, N-terminal domain Length = 541 Score = 27.5 bits (58), Expect = 7.2 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = -1 Query: 76 GERRWWQRW*NTPRPLLDV 20 GE+ WWQ + TPR L DV Sbjct: 241 GEKVWWQEFDVTPRQLEDV 259 >At4g16550.1 68417.m02504 heat shock protein-related contains similarity to Swiss-Prot:P31170 small heat shock protein, chloroplast precursor [Arabidopsis thaliana] Length = 743 Score = 27.5 bits (58), Expect = 7.2 Identities = 18/56 (32%), Positives = 23/56 (41%), Gaps = 2/56 (3%) Frame = +1 Query: 409 GEPVKGSPYAADKRRGYHRQYFPRQGGGRSG--EGVHAEED*DSWTPAPTKGVHKE 570 G G P+A D GY+ P Q G G E + EE D + G+ KE Sbjct: 154 GTATDGFPHATDPNDGYYATNNPYQVNGPKGFTEFKYLEETHDLFVRLDFPGIQKE 209 >At2g47920.1 68415.m05991 kinase interacting family protein similar to kinase interacting protein 1 (GI:13936326) [Petunia integrifolia] Length = 225 Score = 27.5 bits (58), Expect = 7.2 Identities = 12/46 (26%), Positives = 20/46 (43%) Frame = +1 Query: 220 QRQEWIWFHQQDDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFA 357 ++ W WF +K ++ T + KA+ + DG A FA Sbjct: 6 EKSRWWWFESHKSSKHSQWLQSTLAEIDAKTKAMLKLLDGNADSFA 51 >At2g39650.1 68415.m04862 expressed protein contains Pfam profile PF04720: Protein of unknown function (DUF506) Length = 291 Score = 27.5 bits (58), Expect = 7.2 Identities = 20/58 (34%), Positives = 29/58 (50%) Frame = -2 Query: 495 SSATLTREILAVVATALVCSIR*AFYWLTTGTSNTSCFKAFLPGNHGKLHRLSVADRA 322 S A E+L+VV T ++ + + +GT N SC + +L KL RLS D A Sbjct: 73 SMAQHETEVLSVVRTLMLTIKEKDLHSVKSGTCNASCIRFYL----AKLLRLSGYDAA 126 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,788,580 Number of Sequences: 28952 Number of extensions: 230384 Number of successful extensions: 738 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 705 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 736 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1190791976 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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