BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30772.Seq (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch... 89 5e-19 SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 35 0.007 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 28 0.79 SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|... 26 3.2 >SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosaccharomyces pombe|chr 1|||Manual Length = 558 Score = 88.6 bits (210), Expect = 5e-19 Identities = 41/89 (46%), Positives = 55/89 (61%) Frame = +3 Query: 255 HMKCXVFIRPTSENIALLSRELRDPKYGVYFIYFXNXVSKADIKTLAECDEXEAVRXVQE 434 H+KC F+RPT + LL ELRDPKY Y +YF N + K+ ++ LAE D+ EAV+ +QE Sbjct: 63 HLKCVAFLRPTPTTLRLLCEELRDPKYAEYHLYFTNVIPKSFLERLAESDDFEAVKSIQE 122 Query: 435 VFADYLAVDRHLFSFXXVGXLQGSGWNXQ 521 F DYL V+ L SF ++ S N Q Sbjct: 123 FFLDYLVVNNDLASFNIPHIIEDSPDNWQ 151 Score = 37.9 bits (84), Expect = 0.001 Identities = 15/51 (29%), Positives = 35/51 (68%) Frame = +1 Query: 61 MNVIQAVKMYITKMXEXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYL 213 M+++ A + Y ++ + +K++L++++TT IVS +QS +L++++YL Sbjct: 1 MDLVSASQSYFKRIFQEVSD-LKILLLEEDTTKIVSSCITQSNLLEQQIYL 50 >SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces pombe|chr 3|||Manual Length = 639 Score = 35.1 bits (77), Expect = 0.007 Identities = 17/61 (27%), Positives = 32/61 (52%), Gaps = 1/61 (1%) Frame = +3 Query: 273 FIRPTSENIALLSRELRDPKYGVYFIYFXNXVSKADIKTLAE-CDEXEAVRXVQEVFADY 449 F++PT ENI L+ +L Y ++ F + +S+A ++ AE + + +V+ Y Sbjct: 94 FVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALLEQFAELASKTNTSHMIHQVYDQY 153 Query: 450 L 452 L Sbjct: 154 L 154 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 28.3 bits (60), Expect = 0.79 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = -2 Query: 280 RIKTXHFMCSCYPIWHDCLSSRISKLPSVRFP 185 R++ H + SC+P HD S + K P FP Sbjct: 430 RLELLHDVLSCFPKKHDSTSRKKPKFPYQYFP 461 >SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 162 Score = 26.2 bits (55), Expect = 3.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 213 IREDRQSCQMG*HEHMKCXVFIRPTSENIALLSRELRDPKYG 338 IR D C EH + P +E A+L+ +L +PK G Sbjct: 122 IRSDLLKCMS---EHPDKSLICHPLAEKFAILASKLHNPKVG 160 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,945,508 Number of Sequences: 5004 Number of extensions: 34399 Number of successful extensions: 64 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 60 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 64 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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