Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30772.Seq
(548 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu... 71 7e-13
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 32 0.29
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 32 0.29
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ... 28 3.6
At4g37560.1 68417.m05316 formamidase, putative / formamide amido... 27 8.3
At4g37550.1 68417.m05314 formamidase, putative / formamide amido... 27 8.3
At3g11900.1 68416.m01459 amino acid transporter family protein l... 27 8.3
>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
putative / VPS45p, putative identical to vacuolar
protein sorting homolog VPS45p [Arabidopsis thaliana]
gi|2921406|gb|AAC39472
Length = 569
Score = 70.5 bits (165), Expect = 7e-13
Identities = 31/74 (41%), Positives = 48/74 (64%)
Frame = +3
Query: 255 HMKCXVFIRPTSENIALLSRELRDPKYGVYFIYFXNXVSKADIKTLAECDEXEAVRXVQE 434
H+K FIRPTS+NI L +L +P++G Y ++F N + I LA+ DE E V+ VQE
Sbjct: 66 HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125
Query: 435 VFADYLAVDRHLFS 476
+AD+++ D + F+
Sbjct: 126 YYADFVSGDPYHFT 139
Score = 62.1 bits (144), Expect = 2e-10
Identities = 31/57 (54%), Positives = 43/57 (75%)
Frame = +1
Query: 61 MNVIQAVKMYITKMXEXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
M ++ +V+ YI +M + GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1 MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56
>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 662
Score = 31.9 bits (69), Expect = 0.29
Identities = 16/36 (44%), Positives = 21/36 (58%)
Frame = +1
Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
KV++MDK T I+S SEI Q+ + L E I H
Sbjct: 44 KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79
>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 666
Score = 31.9 bits (69), Expect = 0.29
Identities = 18/75 (24%), Positives = 37/75 (49%), Gaps = 3/75 (4%)
Frame = +3
Query: 258 MKCXVFIRPTSENIALLSREL--RDPKYGVYFIYFXNXVSKADIKTL-AECDEXEAVRXV 428
M FI+PT EN+ + ++ + P Y F++F + VSK + + + + +
Sbjct: 86 MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145
Query: 429 QEVFADYLAVDRHLF 473
+E+ ++ A+D F
Sbjct: 146 REMNLEFFAIDSQGF 160
>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
similar to GTP-binding protein GI:303742 from [Pisum
sativum]; contains Pfam profile: PF00071 ras family
Length = 217
Score = 28.3 bits (60), Expect = 3.6
Identities = 15/44 (34%), Positives = 25/44 (56%)
Frame = -1
Query: 473 EQVSVHXQVVSKNFLDLSDSLXFITFGQSFDVCLRNXITEIYKV 342
E +S +V SK LD+ D + GQS +V ++ ++E+ KV
Sbjct: 169 EVLSQIYRVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212
>At4g37560.1 68417.m05316 formamidase, putative / formamide
amidohydrolase, putative similar to SP|Q50228
Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)
{Methylophilus methylotrophus}; contains Pfam profile
PF03069: Acetamidase/Formamidase family
Length = 452
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = -2
Query: 283 GRIKTXHFMCSCYPIWH-DCLSSRISKLPSVRFP 185
G T HF C+ IW+ + + + ++P VRFP
Sbjct: 124 GGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFP 157
>At4g37550.1 68417.m05314 formamidase, putative / formamide
amidohydrolase, putative similar to SP|Q50228
Formamidase (EC 3.5.1.49) (Formamide amidohydrolase)
{Methylophilus methylotrophus}; contains Pfam profile
PF03069: Acetamidase/Formamidase family
Length = 452
Score = 27.1 bits (57), Expect = 8.3
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = -2
Query: 283 GRIKTXHFMCSCYPIWH-DCLSSRISKLPSVRFP 185
G T HF C+ IW+ + + + ++P VRFP
Sbjct: 124 GGFLTDHFPCATKAIWYFEGIYAYSPQIPGVRFP 157
>At3g11900.1 68416.m01459 amino acid transporter family protein low
similarity to proton/amino acid transporter 1 [Mus
musculus] GI:21908024; belongs to INTERPRO:IPR002422
amino acid/polyamine transporter, family II
Length = 432
Score = 27.1 bits (57), Expect = 8.3
Identities = 15/41 (36%), Positives = 24/41 (58%)
Frame = -1
Query: 179 LYTILTMLVVSLSINITFIPGPLSSXILVMYILTACITFIL 57
++T T+LVV L+ + +PG + LV L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,027,860
Number of Sequences: 28952
Number of extensions: 174506
Number of successful extensions: 337
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 333
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 337
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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