BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30735.Seq (618 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 88 8e-19 SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 88 8e-19 SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 26 3.8 SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 26 5.0 SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyc... 25 6.6 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 25 8.8 >SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 88.2 bits (209), Expect = 8e-19 Identities = 56/148 (37%), Positives = 71/148 (47%) Frame = +1 Query: 103 MPPKFDPNEIKIVNLRCVGGEVGATSSLGPKIGPXGLSPKKVGDDIAKATRTGRVSRSLC 282 MPPKFDPNE+K + +R VGGEV S+L PKIGP GLSPKKVG+DIAKAT+ + R Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 283 S*QFKTDKXXXXXXXXXXXXXXXXXXXXXVNVKSRKIXKHQGNIXP*RCIGHCEDS*ETD 462 + + + K K H GN+ I + Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEII-EVARTMRFK 119 Query: 463 SMGPGTFLAQ*KRFFGTAQSVGCTVEGQ 546 S+ K GTA SVGCTV+G+ Sbjct: 120 SLAK-ELSGTVKEILGTAFSVGCTVDGK 146 Score = 80.6 bits (190), Expect = 2e-16 Identities = 44/85 (51%), Positives = 59/85 (69%) Frame = +3 Query: 252 KDWKGLKITVQLTVQNRQAQIAVVPSAAXLIIXALKEPPRERKKQKNXXTPREHXPLKMY 431 KDWKGL++TV+LT+QNRQA ++VVPSA+ L+I ALKEP R+RKK KN + L Sbjct: 51 KDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKN-VAHSGNVSLDEI 109 Query: 432 RALRRFMRNRFNGPRYFSGSVKEIL 506 + R MR + + + SG+VKEIL Sbjct: 110 IEVARTMRFK-SLAKELSGTVKEIL 133 >SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 88.2 bits (209), Expect = 8e-19 Identities = 56/148 (37%), Positives = 71/148 (47%) Frame = +1 Query: 103 MPPKFDPNEIKIVNLRCVGGEVGATSSLGPKIGPXGLSPKKVGDDIAKATRTGRVSRSLC 282 MPPKFDPNE+K + +R VGGEV S+L PKIGP GLSPKKVG+DIAKAT+ + R Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRVTV 60 Query: 283 S*QFKTDKXXXXXXXXXXXXXXXXXXXXXVNVKSRKIXKHQGNIXP*RCIGHCEDS*ETD 462 + + + K K H GN+ I + Sbjct: 61 KLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEII-EVARTMRFK 119 Query: 463 SMGPGTFLAQ*KRFFGTAQSVGCTVEGQ 546 S+ K GTA SVGCTV+G+ Sbjct: 120 SLAK-ELSGTVKEILGTAFSVGCTVDGK 146 Score = 80.6 bits (190), Expect = 2e-16 Identities = 44/85 (51%), Positives = 59/85 (69%) Frame = +3 Query: 252 KDWKGLKITVQLTVQNRQAQIAVVPSAAXLIIXALKEPPRERKKQKNXXTPREHXPLKMY 431 KDWKGL++TV+LT+QNRQA ++VVPSA+ L+I ALKEP R+RKK KN + L Sbjct: 51 KDWKGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKN-VAHSGNVSLDEI 109 Query: 432 RALRRFMRNRFNGPRYFSGSVKEIL 506 + R MR + + + SG+VKEIL Sbjct: 110 IEVARTMRFK-SLAKELSGTVKEIL 133 >SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 26.2 bits (55), Expect = 3.8 Identities = 9/29 (31%), Positives = 16/29 (55%) Frame = -3 Query: 577 VDVINKIMWRPALPQYIQLTVLCQRISFT 491 V ++ K++W LP ++ V+C S T Sbjct: 86 VQLLQKLLWDDGLPALVERKVICTLASLT 114 >SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit Rpa2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1227 Score = 25.8 bits (54), Expect = 5.0 Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 1/59 (1%) Frame = -2 Query: 572 CHQ*DHVAA-CPSTVHPTDCAVPKNLFY*ARKVPGPIESVSHESSQCPIHLQGXMFPWC 399 C +H+A C HP+D + +L P + V HES + L G + WC Sbjct: 568 CGLLNHLARKCEIVTHPSDVSQIPSLLLSLGVDPPSV--VGHESGWGCVQLDGKIVGWC 624 >SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyces pombe|chr 1|||Manual Length = 835 Score = 25.4 bits (53), Expect = 6.6 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = -2 Query: 485 RKVPGPIESVSHESSQCP 432 RKVP P+E++ H S+ P Sbjct: 330 RKVPAPLENIFHIGSKTP 347 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.0 bits (52), Expect = 8.8 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = -2 Query: 182 DDVAPTSPPTHRKFTILI 129 D++A T PP HRK IL+ Sbjct: 249 DELAKTLPPYHRKNLILV 266 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,528,667 Number of Sequences: 5004 Number of extensions: 49796 Number of successful extensions: 127 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 127 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 271646730 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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