BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30733.Seq (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 136 3e-33 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 52 7e-08 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 52 7e-08 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 52 1e-07 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 50 4e-07 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 50 5e-07 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 44 3e-05 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 44 3e-05 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 42 1e-04 SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||... 42 1e-04 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 41 2e-04 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 40 4e-04 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 36 0.007 SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schiz... 31 0.14 SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 30 0.33 SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos... 30 0.44 SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 1.0 SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 1.0 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.8 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.8 SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 27 4.1 SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 26 5.4 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.4 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 9.5 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 25 9.5 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 136 bits (330), Expect = 3e-33 Identities = 62/96 (64%), Positives = 74/96 (77%) Frame = +3 Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVGYDDIGGCRKQL 689 VRG MR VEFKVV+ P F IV+ DT+IH +GEPI RE+EE +L VGYDDIGGCR+Q+ Sbjct: 174 VRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQM 233 Query: 690 AQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 AQI+E+VELPLRHP LFK+IG+KP GP GT Sbjct: 234 AQIRELVELPLRHPQLFKSIGIKPPRGILMYGPPGT 269 Score = 95.9 bits (228), Expect = 6e-21 Identities = 43/100 (43%), Positives = 63/100 (63%) Frame = +1 Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402 + +G+ + IVL+D+ D R+ D+V+I PCP +KY +R+ +L Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137 Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGA 522 P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F G+ Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGS 177 Score = 86.6 bits (205), Expect = 4e-18 Identities = 40/68 (58%), Positives = 52/68 (76%) Frame = +2 Query: 53 NKMADNKSPDDLSTAIXRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232 N D S +D +TAI R+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 233 GKRRKETV 256 GKRRK+TV Sbjct: 81 GKRRKDTV 88 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 52.4 bits (120), Expect = 7e-08 Identities = 20/47 (42%), Positives = 32/47 (68%) Frame = +3 Query: 657 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 Y+ +GG KQ+ +IKE++ELP++HP LF+++G+ GP GT Sbjct: 144 YEMVGGLEKQIKEIKEVIELPVKHPELFESLGIPQPKGILLYGPPGT 190 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 52.4 bits (120), Expect = 7e-08 Identities = 25/65 (38%), Positives = 35/65 (53%) Frame = +3 Query: 603 DGEPIKREEEEEALNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCM 782 D +P+ + + Y DIGG Q+ +IKE VELPL HP L++ +G+KP Sbjct: 172 DTDPMINVMKLDKAPTESYADIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILY 231 Query: 783 GPLGT 797 G GT Sbjct: 232 GAPGT 236 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 52.0 bits (119), Expect = 1e-07 Identities = 21/49 (42%), Positives = 31/49 (63%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 V Y D+GGC++Q+ +++E+VELPL P F +G+ P GP GT Sbjct: 176 VTYGDVGGCKEQIERLREVVELPLLSPERFVKLGIDPPKGIMLYGPPGT 224 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 50.0 bits (114), Expect = 4e-07 Identities = 22/51 (43%), Positives = 32/51 (62%) Frame = +3 Query: 645 NAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 +AV + IGG + Q+AQI+++VELP ++P LFK + P GP GT Sbjct: 274 SAVTFSSIGGLQAQIAQIRDIVELPFQNPELFKFFNIMPPRGVLLYGPPGT 324 Score = 39.5 bits (88), Expect = 5e-04 Identities = 23/58 (39%), Positives = 30/58 (51%) Frame = +3 Query: 621 REEEEEALNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLG 794 RE E+ N V + DIGG + ++KE VE PL H F +GV+P GP G Sbjct: 536 REFMMESPN-VHWSDIGGQEEVKQKLKESVEWPLTHGETFSRLGVRPPKGVLLYGPPG 592 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 49.6 bits (113), Expect = 5e-07 Identities = 18/49 (36%), Positives = 32/49 (65%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 + + +GG +Q+ +++E++ELPL++P LF +G+KP GP GT Sbjct: 129 ISFAGVGGLNEQIRELREVIELPLKNPELFLRVGIKPPKGVLLYGPPGT 177 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 44.0 bits (99), Expect = 3e-05 Identities = 20/49 (40%), Positives = 27/49 (55%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 V Y D+GG Q +++E VELPL L++ IG+ P GP GT Sbjct: 131 VSYADVGGLDVQKQEVREAVELPLTQGDLYRQIGIDPPRGVLLYGPPGT 179 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 44.0 bits (99), Expect = 3e-05 Identities = 19/47 (40%), Positives = 27/47 (57%) Frame = +3 Query: 657 YDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 Y DIGG KQ+ ++ E + LP++ F+ +GVKP GP GT Sbjct: 184 YSDIGGLSKQIEELFEAIVLPMQQADKFRKLGVKPPKGCLMFGPPGT 230 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/51 (33%), Positives = 30/51 (58%) Frame = +3 Query: 645 NAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 +++ ++ +GG + Q+KEMV LPL +P +F+ ++P GP GT Sbjct: 261 SSLSFESVGGLDNYINQLKEMVMLPLLYPEIFQRFNMQPPRGVLFHGPPGT 311 >SPAC17A5.01 |pex6||peroxin-6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 948 Score = 41.5 bits (93), Expect = 1e-04 Identities = 19/49 (38%), Positives = 29/49 (59%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 V +DDIGG + +++ ++LPL+ P LF G+KP + GP GT Sbjct: 652 VNWDDIGGLEEAKTVLRDTLQLPLQFPELFSQ-GLKPRSGVLLYGPPGT 699 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 40.7 bits (91), Expect = 2e-04 Identities = 21/67 (31%), Positives = 33/67 (49%) Frame = +3 Query: 597 HCDGEPIKREEEEEALNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFS 776 H D + I+ + + ++ IGG + Q+KEMV LPL +P +F + + P Sbjct: 351 HIDEKTIRSTDPFANRENLDFNSIGGLEDIILQLKEMVMLPLLYPEVFLHLHITPPRGVL 410 Query: 777 CMGPLGT 797 GP GT Sbjct: 411 FHGPPGT 417 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 39.9 bits (89), Expect = 4e-04 Identities = 17/59 (28%), Positives = 30/59 (50%) Frame = +3 Query: 618 KREEEEEALNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLG 794 K E E + + DIGG + ++ E+V +P++HP +++ G+ P GP G Sbjct: 160 KAEANREPPSDISLSDIGGLDDCINELLELVAMPIKHPEVYQYTGIHPPRGVLLHGPPG 218 Score = 31.9 bits (69), Expect = 0.11 Identities = 12/62 (19%), Positives = 30/62 (48%) Frame = +3 Query: 609 EPIKREEEEEALNAVGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGP 788 +P + E + V +++IG + +++ + P++ P L++++G+ GP Sbjct: 475 QPSSKREGFATVPGVSWNNIGALKSIRVELQMAIVQPIKRPELYQSVGISAPTGVLLWGP 534 Query: 789 LG 794 G Sbjct: 535 PG 536 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 35.9 bits (79), Expect = 0.007 Identities = 20/49 (40%), Positives = 26/49 (53%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 V +DDI G +KE V LP++ P LF + G KP + GP GT Sbjct: 126 VRWDDIAGLENAKEALKETVLLPIKLPQLF-SHGRKPWSGILLYGPPGT 173 >SPCC24B10.10c |||mitochondrial outer membrane ATPase Msp1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 355 Score = 31.5 bits (68), Expect = 0.14 Identities = 13/34 (38%), Positives = 21/34 (61%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIG 752 V +DDIGG + + Q+ + V PL++P +F G Sbjct: 86 VSFDDIGGMDEHVNQLLQDVLFPLKYPEVFDTHG 119 >SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 641 Score = 30.3 bits (65), Expect = 0.33 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Frame = -1 Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384 DH L +H PA+ LK I A++ G T +PV P+ ++G + TRF Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410 >SPAC328.04 |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 1|||Manual Length = 741 Score = 29.9 bits (64), Expect = 0.44 Identities = 17/49 (34%), Positives = 22/49 (44%) Frame = +3 Query: 651 VGYDDIGGCRKQLAQIKEMVELPLRHPSLFKAIGVKPHAAFSCMGPLGT 797 V +DDI G +KE V P P LF+ + +P GP GT Sbjct: 456 VHWDDISGLEFAKHSLKEAVVYPFLRPDLFQGLR-EPARGMLLFGPPGT 503 >SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 27.9 bits (59), Expect = 1.8 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +3 Query: 78 LMIYRPRSXVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281 LV + LKD WI + + E F G ++FS+QG Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472 >SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein S0B|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 26.6 bits (56), Expect = 4.1 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +3 Query: 576 VAPDTVIHCDGEPIKREEEEEALNAVGYDD 665 V PD + D E I+REEE++A A ++ Sbjct: 200 VMPDLYFYRDPEEIEREEEQKAAAAAAAEE 229 >SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 26.2 bits (55), Expect = 5.4 Identities = 14/48 (29%), Positives = 21/48 (43%) Frame = -2 Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248 L FH ++ D W PH+ + G F H + +G + R GF Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 26.2 bits (55), Expect = 5.4 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAIXRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTK-SKRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.4 bits (53), Expect = 9.5 Identities = 10/38 (26%), Positives = 22/38 (57%) Frame = +2 Query: 137 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRKE 250 E+AV D+N+ ++L+ + + RGD ++ G ++ Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERD 284 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.4 bits (53), Expect = 9.5 Identities = 14/35 (40%), Positives = 23/35 (65%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAIXRRKDRPNRLIVEEAVSD 154 LNK+ D+ D + I R++D PNRL++ A+S+ Sbjct: 227 LNKI-DHPDADANISKIARKED-PNRLVLASAISE 259 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,291,332 Number of Sequences: 5004 Number of extensions: 65806 Number of successful extensions: 203 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 194 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 203 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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