Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30729.Seq
         (548 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch...    93   2e-20
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ...    40   2e-04
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    29   0.34 
SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|...    26   4.2  
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc...    25   9.7  
SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr 2|...    25   9.7  

>SPAC2G11.03c |vps45||vacuolar sorting protein Vps
           45|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 558

 Score = 93.5 bits (222), Expect = 2e-20
 Identities = 40/77 (51%), Positives = 54/77 (70%)
 Frame = +3

Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEXXAVREVQE 434
           H+KC+ F+RPT   + LL  ELRDPKY  Y +YF+NV+ K+ ++ LAE D+  AV+ +QE
Sbjct: 63  HLKCVAFLRPTPTTLRLLCEELRDPKYAEYHLYFTNVIPKSFLERLAESDDFEAVKSIQE 122

Query: 435 VFADYLAVDRHLFSFNI 485
            F DYL V+  L SFNI
Sbjct: 123 FFLDYLVVNNDLASFNI 139



 Score = 36.7 bits (81), Expect = 0.002
 Identities = 15/51 (29%), Positives = 34/51 (66%)
 Frame = +1

Query: 61  MNVIQAVKMYITKMXXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYL 213
           M+++ A + Y  ++       +K++L++++TT IVS   +QS +L++++YL
Sbjct: 1   MDLVSASQSYFKRIFQEVSD-LKILLLEEDTTKIVSSCITQSNLLEQQIYL 50


>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 639

 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 20/77 (25%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
 Frame = +3

Query: 267 IVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAE----CDEXXAVREVQE 434
           I F++PT ENI L+  +L    Y   ++ FS+ +S+A ++  AE     +    + +V +
Sbjct: 92  IYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALLEQFAELASKTNTSHMIHQVYD 151

Query: 435 VFADYLAVDRHLFSFNI 485
            + +Y+ ++   FS  +
Sbjct: 152 QYLNYVVLESDFFSLQL 168


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2685

 Score = 29.5 bits (63), Expect = 0.34
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -2

Query: 280 RIKTMHFMCSCYPIWHDCLSSRISKLPSVRFP 185
           R++ +H + SC+P  HD  S +  K P   FP
Sbjct: 430 RLELLHDVLSCFPKKHDSTSRKKPKFPYQYFP 461


>SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 162

 Score = 25.8 bits (54), Expect = 4.2
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +3

Query: 213 IREDRQSCQMG*HEHMKCIVFIRPTSENIALLSRELRDPKYG 338
           IR D   C     EH    +   P +E  A+L+ +L +PK G
Sbjct: 122 IRSDLLKCMS---EHPDKSLICHPLAEKFAILASKLHNPKVG 160


>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 762

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 20/72 (27%), Positives = 31/72 (43%)
 Frame = -1

Query: 548 ADLEKLVGDAVGSNPTXEASNNIEREQVSVHSQVVSKNFLDLSDSXXFITFGQSFDVCLR 369
           ADL+ +   A       E   N+  +  SV  ++++ N   +      + F  SFDV  R
Sbjct: 321 ADLQLIDKMANAPKHVVEFLENLSLKNSSVLKKILN-NLALMKKKELNLNFLPSFDVWDR 379

Query: 368 NYITEIYKVNTI 333
            Y T  YK + I
Sbjct: 380 EYYTARYKQSLI 391


>SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 482

 Score = 24.6 bits (51), Expect = 9.7
 Identities = 10/29 (34%), Positives = 18/29 (62%)
 Frame = -1

Query: 539 EKLVGDAVGSNPTXEASNNIEREQVSVHS 453
           + L+ + +G+N     SN+I R + S+HS
Sbjct: 9   QHLLANTLGNNDENHPSNHIARAKSSLHS 37


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,213,088
Number of Sequences: 5004
Number of extensions: 42357
Number of successful extensions: 98
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 98
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 227943826
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)