Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30729.Seq
         (548 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu...    77   1e-14
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat...    36   0.024
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE...    36   0.024
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ...    29   2.0  
At3g11900.1 68416.m01459 amino acid transporter family protein l...    27   6.2  

>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
           putative / VPS45p, putative identical to vacuolar
           protein sorting homolog VPS45p [Arabidopsis thaliana]
           gi|2921406|gb|AAC39472
          Length = 569

 Score = 76.6 bits (180), Expect = 1e-14
 Identities = 32/77 (41%), Positives = 53/77 (68%)
 Frame = +3

Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEXXAVREVQE 434
           H+K + FIRPTS+NI  L  +L +P++G Y ++FSN++    I  LA+ DE   V++VQE
Sbjct: 66  HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125

Query: 435 VFADYLAVDRHLFSFNI 485
            +AD+++ D + F+ N+
Sbjct: 126 YYADFVSGDPYHFTLNM 142



 Score = 60.9 bits (141), Expect = 5e-10
 Identities = 31/57 (54%), Positives = 42/57 (73%)
 Frame = +1

Query: 61  MNVIQAVKMYITKMXXXSGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
           M ++ +V+ YI +M      GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1   MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56


>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 662

 Score = 35.5 bits (78), Expect = 0.024
 Identities = 20/78 (25%), Positives = 42/78 (53%), Gaps = 3/78 (3%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXXAVREV 428
           M+ I FI+PT EN+     ++  + P Y   F++FS+ VS++ +  +  +      +  +
Sbjct: 86  MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145

Query: 429 QEVFADYLAVDRHLFSFN 482
           +E+  +Y+++D   F  N
Sbjct: 146 KEMNLEYISMDIQGFVTN 163



 Score = 31.9 bits (69), Expect = 0.29
 Identities = 16/36 (44%), Positives = 21/36 (58%)
 Frame = +1

Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
           KV++MDK T  I+S     SEI Q+ + L E I  H
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79


>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 666

 Score = 35.5 bits (78), Expect = 0.024
 Identities = 20/75 (26%), Positives = 39/75 (52%), Gaps = 3/75 (4%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEXXAVREV 428
           M  I FI+PT EN+ +   ++  + P Y   F++FS+ VSK  +  +  +      +  +
Sbjct: 86  MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145

Query: 429 QEVFADYLAVDRHLF 473
           +E+  ++ A+D   F
Sbjct: 146 REMNLEFFAIDSQGF 160


>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
           similar to GTP-binding protein GI:303742 from [Pisum
           sativum]; contains Pfam profile: PF00071 ras family
          Length = 217

 Score = 29.1 bits (62), Expect = 2.0
 Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
 Frame = -1

Query: 485 NIEREQVSVHSQ---VVSKNFLDLSDSXXFITFGQSFDVCLRNYITEIYKV 342
           N+E     V SQ   V SK  LD+ D    +  GQS +V  ++ ++E+ KV
Sbjct: 162 NVENAFTEVLSQIYRVASKKALDIGDDHTTLPKGQSINVGSKDDVSEVKKV 212


>At3g11900.1 68416.m01459 amino acid transporter family protein low
           similarity to proton/amino acid transporter 1 [Mus
           musculus] GI:21908024; belongs to INTERPRO:IPR002422
           amino acid/polyamine transporter, family II
          Length = 432

 Score = 27.5 bits (58), Expect = 6.2
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -1

Query: 179 LYTILTMLVVSLSINITFIPGPLSXXILVMYILTACITFIL 57
           ++T  T+LVV L+   + +PG  +   LV   L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,221,811
Number of Sequences: 28952
Number of extensions: 208234
Number of successful extensions: 470
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 463
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 469
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1033331880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)