Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30728.Seq
(698 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 120 2e-28
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 120 2e-28
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 120 2e-28
SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces po... 25 7.9
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 120 bits (289), Expect = 2e-28
Identities = 66/138 (47%), Positives = 78/138 (56%), Gaps = 2/138 (1%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDRXXSGTCLWKLRHCDWTQS* 435
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK DR G +
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRS--SGNYVIIVGHD 139
Query: 436 C*AYKSKATVWXQEGSAIKQQMHG--RIVAGGGRIDKPILKAGRGYHKYKVKRNXWPICX 609
K++ + + G IVAGGGRIDKP+LKAGR +HKY+VKRN WP
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 610 XCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 200 GVAMNPV-DHPHGGGNHQ 216
Score = 118 bits (283), Expect = 1e-27
Identities = 54/83 (65%), Positives = 69/83 (83%)
Frame = +3
Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
V FR+PY ++T E F+A E ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 57.2 bits (132), Expect = 2e-09
Identities = 25/41 (60%), Positives = 33/41 (80%)
Frame = +2
Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMV 508
L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PSS R +V
Sbjct: 125 LGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVV 165
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 120 bits (289), Expect = 2e-28
Identities = 66/138 (47%), Positives = 78/138 (56%), Gaps = 2/138 (1%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDRXXSGTCLWKLRHCDWTQS* 435
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK DR G +
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRS--SGNYVIIVGHD 139
Query: 436 C*AYKSKATVWXQEGSAIKQQMHG--RIVAGGGRIDKPILKAGRGYHKYKVKRNXWPICX 609
K++ + + G IVAGGGRIDKP+LKAGR +HKY+VKRN WP
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 610 XCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 200 GVAMNPV-DHPHGGGNHQ 216
Score = 118 bits (283), Expect = 1e-27
Identities = 54/83 (65%), Positives = 69/83 (83%)
Frame = +3
Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
V FR+PY ++T E F+A E ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 57.2 bits (132), Expect = 2e-09
Identities = 25/41 (60%), Positives = 33/41 (80%)
Frame = +2
Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMV 508
L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PSS R +V
Sbjct: 125 LGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVV 165
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 120 bits (289), Expect = 2e-28
Identities = 66/138 (47%), Positives = 78/138 (56%), Gaps = 2/138 (1%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDRXXSGTCLWKLRHCDWTQS* 435
YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK DR G +
Sbjct: 82 YTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRS--SGNYVIIVGHD 139
Query: 436 C*AYKSKATVWXQEGSAIKQQMHG--RIVAGGGRIDKPILKAGRGYHKYKVKRNXWPICX 609
K++ + + G IVAGGGRIDKP+LKAGR +HKY+VKRN WP
Sbjct: 140 VDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTR 199
Query: 610 XCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 200 GVAMNPV-DHPHGGGNHQ 216
Score = 118 bits (283), Expect = 1e-27
Identities = 54/83 (65%), Positives = 69/83 (83%)
Frame = +3
Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
V FR+PY ++T E F+A E ++
Sbjct: 60 VAFRNPYHYRTDVETFVATEGMY 82
Score = 57.2 bits (132), Expect = 2e-09
Identities = 25/41 (60%), Positives = 33/41 (80%)
Frame = +2
Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMV 508
L R+SGN+ ++GH+ D +TRVKLPSGAKKV+PSS R +V
Sbjct: 125 LGRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVV 165
>SPCC622.11 |||LMBR1-like membrane protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 562
Score = 25.4 bits (53), Expect = 7.9
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +3
Query: 507 SYCCWRWTY*QTYFESWKGLPQVQG 581
S WR+ Y T+ SW LP VQG
Sbjct: 116 SLSIWRFLYWLTFVLSWIILPFVQG 140
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,694,387
Number of Sequences: 5004
Number of extensions: 51710
Number of successful extensions: 146
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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