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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30728.Seq
         (698 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_19257| Best HMM Match : No HMM Matches (HMM E-Value=.)             142   2e-34
SB_42692| Best HMM Match : Ribosomal_L2_C (HMM E-Value=4.9e-05)        73   3e-13
SB_18491| Best HMM Match : Keratin_B2 (HMM E-Value=0.64)               31   0.68 
SB_44978| Best HMM Match : REJ (HMM E-Value=3.9e-13)                   29   4.8  
SB_50339| Best HMM Match : TNFR_c6 (HMM E-Value=0.0064)                28   8.4  

>SB_19257| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 260

 Score =  142 bits (344), Expect = 2e-34
 Identities = 65/83 (78%), Positives = 72/83 (86%)
 Frame = +3

Query: 12  MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
           MGRVIR QRKGAGS+F SHTK RKGA  LR  DYAERHGYIKGVVK+IIHDPGRGAPLAV
Sbjct: 1   MGRVIRGQRKGAGSIFTSHTKHRKGAANLRVHDYAERHGYIKGVVKEIIHDPGRGAPLAV 60

Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
           V FRDPY++K RKELF+A E ++
Sbjct: 61  VVFRDPYRYKLRKELFVATEGMY 83



 Score =  117 bits (282), Expect = 8e-27
 Identities = 65/140 (46%), Positives = 80/140 (57%), Gaps = 4/140 (2%)
 Frame = +1

Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR----XXSGTCLWKLRHCDW 423
           YTGQF+YCGKKA L++GN +PVG MPEGTI+ ++EEK  DR      SG     + H   
Sbjct: 83  YTGQFIYCGKKAALQIGNCLPVGIMPEGTIISSVEEKTGDRGRLARTSGNYATVISHNVE 142

Query: 424 TQS*C*AYKSKATVWXQEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPI 603
            +      + K     ++      +    IVAGGGRIDKP+LKAGR YHKYK KRN WP 
Sbjct: 143 KK----RTRVKLPSGIKKVIPSSNRALVGIVAGGGRIDKPMLKAGRAYHKYKAKRNCWPR 198

Query: 604 CXXCCPXTL*SXLHGGGNHQ 663
                   +    HGGGNHQ
Sbjct: 199 VRGVAMNPV-EHPHGGGNHQ 217



 Score = 69.7 bits (163), Expect = 2e-12
 Identities = 39/81 (48%), Positives = 48/81 (59%)
 Frame = +2

Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMVVLLLEVDVLTNLF*KLEGA 565
           LAR SGN+ATVI HN + KRTRVKLPSG KKV+PSSNR +V ++     +     K   A
Sbjct: 126 LARTSGNYATVISHNVEKKRTRVKLPSGIKKVIPSSNRALVGIVAGGGRIDKPMLKAGRA 185

Query: 566 TTSTRSNVTXGPYVRXVAHXP 628
               ++     P VR VA  P
Sbjct: 186 YHKYKAKRNCWPRVRGVAMNP 206


>SB_42692| Best HMM Match : Ribosomal_L2_C (HMM E-Value=4.9e-05)
          Length = 123

 Score = 72.5 bits (170), Expect = 3e-13
 Identities = 31/41 (75%), Positives = 37/41 (90%)
 Frame = +3

Query: 138 GVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEXLH 260
           GVVK+IIHDPGRGAPLAVV FRDPY++K RKELF+A E ++
Sbjct: 1   GVVKEIIHDPGRGAPLAVVVFRDPYRYKLRKELFVATEGMY 41



 Score = 71.3 bits (167), Expect = 7e-13
 Identities = 28/41 (68%), Positives = 35/41 (85%)
 Frame = +1

Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR 378
           YTGQF+YCGKKA L++GN +PVG MPEGTI+ ++EEK  DR
Sbjct: 41  YTGQFIYCGKKAALQIGNCLPVGIMPEGTIISSVEEKTGDR 81



 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 30/40 (75%), Positives = 34/40 (85%)
 Frame = +2

Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCM 505
           LAR SGN+ATVI HN + KRTRVKLPSG KKV+PSSNR +
Sbjct: 84  LARTSGNYATVISHNVEKKRTRVKLPSGIKKVIPSSNRAL 123


>SB_18491| Best HMM Match : Keratin_B2 (HMM E-Value=0.64)
          Length = 209

 Score = 31.5 bits (68), Expect = 0.68
 Identities = 16/62 (25%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
 Frame = -1

Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH--TMVPSGIAPTGITFPTSRVA-FFPQ*TN 267
           + DC  S     + H PD  +S ++ +R+H      SG+ PT + +P  +++  +P   +
Sbjct: 64  YPDCQMSGVHPTRVHYPDCQMSGVYPTRVHYPDCQMSGVHPTRVHYPDCQMSGVYPTRVH 123

Query: 266 WP 261
           +P
Sbjct: 124 YP 125



 Score = 28.7 bits (61), Expect = 4.8
 Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 4/63 (6%)
 Frame = -1

Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH---TMVPSGIAPTGITFPTSRVA-FFPQ*T 270
           + DC  S       H PD  +S ++ +R+H     +P G+ PT + +P  +++  +P   
Sbjct: 4   YPDCQMSGVHPTGVHYPDCQMSGVYPTRVHYPDCQMP-GVHPTRVHYPDCQMSGVYPTRV 62

Query: 269 NWP 261
           ++P
Sbjct: 63  HYP 65



 Score = 27.9 bits (59), Expect = 8.4
 Identities = 14/52 (26%), Positives = 26/52 (50%), Gaps = 2/52 (3%)
 Frame = -1

Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH--TMVPSGIAPTGITFPTSRVA 288
           + DC  S     + H PD  +S +  +R+H      SG+ PT + +P  +++
Sbjct: 49  YPDCQMSGVYPTRVHYPDCQMSGVHPTRVHYPDCQMSGVYPTRVHYPDCQMS 100


>SB_44978| Best HMM Match : REJ (HMM E-Value=3.9e-13)
          Length = 1819

 Score = 28.7 bits (61), Expect = 4.8
 Identities = 20/47 (42%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
 Frame = -3

Query: 585 FDLVLVVAPSSFQNRF--VXXXXXXXXXTMHLLLDGRTFLAPDGSFT 451
           F L   V PSS QN F             ++L+LD  TFL PDGS T
Sbjct: 114 FSLNCDVIPSS-QNAFQSFEFNPAATTTYLNLILDSETFLEPDGSAT 159


>SB_50339| Best HMM Match : TNFR_c6 (HMM E-Value=0.0064)
          Length = 416

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = +1

Query: 550 KAGRGYHKYKVKRNXWPICXXCCP 621
           K  RGY++  +  + WP C  CCP
Sbjct: 165 KCKRGYYQEPIVYDCWP-CSKCCP 187


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,211,739
Number of Sequences: 59808
Number of extensions: 412530
Number of successful extensions: 887
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 820
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 882
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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