BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30728.Seq (698 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_19257| Best HMM Match : No HMM Matches (HMM E-Value=.) 142 2e-34 SB_42692| Best HMM Match : Ribosomal_L2_C (HMM E-Value=4.9e-05) 73 3e-13 SB_18491| Best HMM Match : Keratin_B2 (HMM E-Value=0.64) 31 0.68 SB_44978| Best HMM Match : REJ (HMM E-Value=3.9e-13) 29 4.8 SB_50339| Best HMM Match : TNFR_c6 (HMM E-Value=0.0064) 28 8.4 >SB_19257| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 260 Score = 142 bits (344), Expect = 2e-34 Identities = 65/83 (78%), Positives = 72/83 (86%) Frame = +3 Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191 MGRVIR QRKGAGS+F SHTK RKGA LR DYAERHGYIKGVVK+IIHDPGRGAPLAV Sbjct: 1 MGRVIRGQRKGAGSIFTSHTKHRKGAANLRVHDYAERHGYIKGVVKEIIHDPGRGAPLAV 60 Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260 V FRDPY++K RKELF+A E ++ Sbjct: 61 VVFRDPYRYKLRKELFVATEGMY 83 Score = 117 bits (282), Expect = 8e-27 Identities = 65/140 (46%), Positives = 80/140 (57%), Gaps = 4/140 (2%) Frame = +1 Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR----XXSGTCLWKLRHCDW 423 YTGQF+YCGKKA L++GN +PVG MPEGTI+ ++EEK DR SG + H Sbjct: 83 YTGQFIYCGKKAALQIGNCLPVGIMPEGTIISSVEEKTGDRGRLARTSGNYATVISHNVE 142 Query: 424 TQS*C*AYKSKATVWXQEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPI 603 + + K ++ + IVAGGGRIDKP+LKAGR YHKYK KRN WP Sbjct: 143 KK----RTRVKLPSGIKKVIPSSNRALVGIVAGGGRIDKPMLKAGRAYHKYKAKRNCWPR 198 Query: 604 CXXCCPXTL*SXLHGGGNHQ 663 + HGGGNHQ Sbjct: 199 VRGVAMNPV-EHPHGGGNHQ 217 Score = 69.7 bits (163), Expect = 2e-12 Identities = 39/81 (48%), Positives = 48/81 (59%) Frame = +2 Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMVVLLLEVDVLTNLF*KLEGA 565 LAR SGN+ATVI HN + KRTRVKLPSG KKV+PSSNR +V ++ + K A Sbjct: 126 LARTSGNYATVISHNVEKKRTRVKLPSGIKKVIPSSNRALVGIVAGGGRIDKPMLKAGRA 185 Query: 566 TTSTRSNVTXGPYVRXVAHXP 628 ++ P VR VA P Sbjct: 186 YHKYKAKRNCWPRVRGVAMNP 206 >SB_42692| Best HMM Match : Ribosomal_L2_C (HMM E-Value=4.9e-05) Length = 123 Score = 72.5 bits (170), Expect = 3e-13 Identities = 31/41 (75%), Positives = 37/41 (90%) Frame = +3 Query: 138 GVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELFIAPEXLH 260 GVVK+IIHDPGRGAPLAVV FRDPY++K RKELF+A E ++ Sbjct: 1 GVVKEIIHDPGRGAPLAVVVFRDPYRYKLRKELFVATEGMY 41 Score = 71.3 bits (167), Expect = 7e-13 Identities = 28/41 (68%), Positives = 35/41 (85%) Frame = +1 Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR 378 YTGQF+YCGKKA L++GN +PVG MPEGTI+ ++EEK DR Sbjct: 41 YTGQFIYCGKKAALQIGNCLPVGIMPEGTIISSVEEKTGDR 81 Score = 66.5 bits (155), Expect = 2e-11 Identities = 30/40 (75%), Positives = 34/40 (85%) Frame = +2 Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCM 505 LAR SGN+ATVI HN + KRTRVKLPSG KKV+PSSNR + Sbjct: 84 LARTSGNYATVISHNVEKKRTRVKLPSGIKKVIPSSNRAL 123 >SB_18491| Best HMM Match : Keratin_B2 (HMM E-Value=0.64) Length = 209 Score = 31.5 bits (68), Expect = 0.68 Identities = 16/62 (25%), Positives = 32/62 (51%), Gaps = 3/62 (4%) Frame = -1 Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH--TMVPSGIAPTGITFPTSRVA-FFPQ*TN 267 + DC S + H PD +S ++ +R+H SG+ PT + +P +++ +P + Sbjct: 64 YPDCQMSGVHPTRVHYPDCQMSGVYPTRVHYPDCQMSGVHPTRVHYPDCQMSGVYPTRVH 123 Query: 266 WP 261 +P Sbjct: 124 YP 125 Score = 28.7 bits (61), Expect = 4.8 Identities = 16/63 (25%), Positives = 32/63 (50%), Gaps = 4/63 (6%) Frame = -1 Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH---TMVPSGIAPTGITFPTSRVA-FFPQ*T 270 + DC S H PD +S ++ +R+H +P G+ PT + +P +++ +P Sbjct: 4 YPDCQMSGVHPTGVHYPDCQMSGVYPTRVHYPDCQMP-GVHPTRVHYPDCQMSGVYPTRV 62 Query: 269 NWP 261 ++P Sbjct: 63 HYP 65 Score = 27.9 bits (59), Expect = 8.4 Identities = 14/52 (26%), Positives = 26/52 (50%), Gaps = 2/52 (3%) Frame = -1 Query: 437 HQDCVQSQWRSFQRHVPDXXLSXIFSSRLH--TMVPSGIAPTGITFPTSRVA 288 + DC S + H PD +S + +R+H SG+ PT + +P +++ Sbjct: 49 YPDCQMSGVYPTRVHYPDCQMSGVHPTRVHYPDCQMSGVYPTRVHYPDCQMS 100 >SB_44978| Best HMM Match : REJ (HMM E-Value=3.9e-13) Length = 1819 Score = 28.7 bits (61), Expect = 4.8 Identities = 20/47 (42%), Positives = 23/47 (48%), Gaps = 2/47 (4%) Frame = -3 Query: 585 FDLVLVVAPSSFQNRF--VXXXXXXXXXTMHLLLDGRTFLAPDGSFT 451 F L V PSS QN F ++L+LD TFL PDGS T Sbjct: 114 FSLNCDVIPSS-QNAFQSFEFNPAATTTYLNLILDSETFLEPDGSAT 159 >SB_50339| Best HMM Match : TNFR_c6 (HMM E-Value=0.0064) Length = 416 Score = 27.9 bits (59), Expect = 8.4 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = +1 Query: 550 KAGRGYHKYKVKRNXWPICXXCCP 621 K RGY++ + + WP C CCP Sbjct: 165 KCKRGYYQEPIVYDCWP-CSKCCP 187 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,211,739 Number of Sequences: 59808 Number of extensions: 412530 Number of successful extensions: 887 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 820 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 882 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1829596184 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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