Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30728.Seq
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 128 3e-30
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 128 3e-30
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 120 7e-28
At5g56980.1 68418.m07112 expressed protein non-consensus CG dono... 29 3.0
At5g65290.1 68418.m08212 LMBR1 integral membrane family protein ... 28 6.8
At3g24090.1 68416.m03025 glucosamine--fructose-6-phosphate amino... 27 9.0
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 128 bits (309), Expect = 3e-30
Identities = 59/83 (71%), Positives = 69/83 (83%)
Frame = +3
Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
V FR P++FK +KELF+A E ++
Sbjct: 61 VAFRHPFRFKKQKELFVAAEGMY 83
Score = 108 bits (260), Expect = 3e-24
Identities = 62/140 (44%), Positives = 79/140 (56%), Gaps = 4/140 (2%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR----XXSGTCLWKLRHCDW 423
YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E DR SG + H
Sbjct: 83 YTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFARASGDYAIVIAHNPD 142
Query: 424 TQS*C*AYKSKATVWXQEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPI 603
+ S + G + M G+ VAGGGR +KP+LKAG YHKY+VKRN WP
Sbjct: 143 NDTSRIKLPSGSKKIVPSGC---RAMIGQ-VAGGGRTEKPMLKAGNAYHKYRVKRNCWPK 198
Query: 604 CXXCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 199 VRGVAMNPV-EHPHGGGNHQ 217
Score = 57.2 bits (132), Expect = 1e-08
Identities = 24/40 (60%), Positives = 32/40 (80%)
Frame = +2
Query: 389 ARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMV 508
ARASG++A VI HNPD +R+KLPSG+KK++PS R M+
Sbjct: 127 ARASGDYAIVIAHNPDNDTSRIKLPSGSKKIVPSGCRAMI 166
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 128 bits (309), Expect = 3e-30
Identities = 59/83 (71%), Positives = 69/83 (83%)
Frame = +3
Query: 12 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 191
MGRVIRAQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLAR 60
Query: 192 VHFRDPYKFKTRKELFIAPEXLH 260
V FR P++FK +KELF+A E ++
Sbjct: 61 VTFRHPFRFKKQKELFVAAEGMY 83
Score = 109 bits (262), Expect = 2e-24
Identities = 63/140 (45%), Positives = 80/140 (57%), Gaps = 4/140 (2%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR----XXSGTCLWKLRHCDW 423
YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E DR SG + H
Sbjct: 83 YTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVGDRGVLARASGDYAIVIAHNPD 142
Query: 424 TQS*C*AYKSKATVWXQEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPI 603
+ + S + G + M G+ VAGGGR +KP+LKAG YHKY+VKRN WP
Sbjct: 143 SDTTRIKLPSGSKKIVPSGC---RAMIGQ-VAGGGRTEKPMLKAGNAYHKYRVKRNSWPK 198
Query: 604 CXXCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 199 VRGVAMNPV-EHPHGGGNHQ 217
Score = 61.7 bits (143), Expect = 5e-10
Identities = 26/41 (63%), Positives = 34/41 (82%)
Frame = +2
Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMV 508
LARASG++A VI HNPD+ TR+KLPSG+KK++PS R M+
Sbjct: 126 LARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMI 166
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 120 bits (290), Expect = 7e-28
Identities = 57/84 (67%), Positives = 68/84 (80%), Gaps = 1/84 (1%)
Frame = +3
Query: 12 MGRVIRAQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLA 188
MGRVIRAQRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLA 60
Query: 189 VVHFRDPYKFKTRKELFIAPEXLH 260
V FR P+++ +KELF+A E ++
Sbjct: 61 RVAFRHPFRYMKQKELFVAAEGMY 84
Score = 108 bits (259), Expect = 4e-24
Identities = 63/140 (45%), Positives = 80/140 (57%), Gaps = 4/140 (2%)
Frame = +1
Query: 256 YTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKXSDR----XXSGTCLWKLRHCDW 423
YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E DR SG + H
Sbjct: 84 YTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALARASGDYAIVIAHN-- 141
Query: 424 TQS*C*AYKSKATVWXQEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPI 603
+S K + SA + M G+ VAGGGR +KP LKAG YHKYK KRN WP+
Sbjct: 142 PESNTTRVKLPSGSKKILPSACR-AMIGQ-VAGGGRTEKPFLKAGNAYHKYKAKRNCWPV 199
Query: 604 CXXCCPXTL*SXLHGGGNHQ 663
+ HGGGNHQ
Sbjct: 200 VRGVAMNPV-EHPHGGGNHQ 218
Score = 64.9 bits (151), Expect = 5e-11
Identities = 36/81 (44%), Positives = 47/81 (58%)
Frame = +2
Query: 386 LARASGNFATVIGHNPDAKRTRVKLPSGAKKVLPSSNRCMVVLLLEVDVLTNLF*KLEGA 565
LARASG++A VI HNP++ TRVKLPSG+KK+LPS+ R M+ + F K A
Sbjct: 127 LARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEKPFLKAGNA 186
Query: 566 TTSTRSNVTXGPYVRXVAHXP 628
++ P VR VA P
Sbjct: 187 YHKYKAKRNCWPVVRGVAMNP 207
>At5g56980.1 68418.m07112 expressed protein non-consensus CG donor
splice site at exon 1, GA donor splice site at exon 3
Length = 379
Score = 29.1 bits (62), Expect = 3.0
Identities = 15/42 (35%), Positives = 19/42 (45%)
Frame = -3
Query: 435 SGLCPITVAKFPEARARXRSIRXFLFKIAHNGTLRHSSNRHH 310
SG P +A+ P R +SI L+K H T S HH
Sbjct: 49 SGHAPAPLARAPSIIDRVKSINFHLYKFPHPETELFSMTAHH 90
>At5g65290.1 68418.m08212 LMBR1 integral membrane family protein
contains Pfam PF04791: LMBR1-like conserved region
Length = 733
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +3
Query: 486 HQATDAWSYCCWRWTY*QTYFESWKGLPQVQG 581
H A S+ W W+Y T+ +W +P +QG
Sbjct: 64 HPENGAISFL-WSWSYWSTFLLTWAVVPLIQG 94
>At3g24090.1 68416.m03025 glucosamine--fructose-6-phosphate
aminotransferase [isomerizing], putative /
hexosephosphate aminotransferase, putative /
glucosamine-6-phosphate synthase, putative /
D-fructose-6-phosphate amidotransferase, putative /
GLCN6P synthase, putative similar to SP|O94808
Glucosamine--fructose-6-phosphate aminotransferase
[isomerizing] 2 (EC 2.6.1.16) (Hexosephosphate
aminotransferase 2) (D-fructose-6- phosphate
amidotransferase 2) {Homo sapiens}; contains Pfam
profiles PF00310: Glutamine amidotransferases class-II,
PF01380:SIS domain
Length = 691
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/48 (25%), Positives = 23/48 (47%)
Frame = +1
Query: 472 QEGSAIKQQMHGRIVAGGGRIDKPILKAGRGYHKYKVKRNXWPICXXC 615
++ ++ M GR++ GG R K +L G H ++R+ + C
Sbjct: 335 EQPESLTTTMRGRLIRGGSRKTKTVLLGGLKDHLKTIRRSRRIVFIGC 382
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,616,663
Number of Sequences: 28952
Number of extensions: 286843
Number of successful extensions: 680
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 661
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 676
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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