Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30693.Seq
(748 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep... 146 4e-34
UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella ve... 83 5e-15
UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling p... 75 2e-12
UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin ... 74 4e-12
UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whol... 73 8e-12
UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo sa... 69 2e-10
UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whol... 61 2e-08
UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus purpura... 53 9e-06
UniRef50_UPI00005846A8 Cluster: PREDICTED: hypothetical protein;... 50 8e-05
UniRef50_Q5C7Y0 Cluster: SJCHGC09066 protein; n=1; Schistosoma j... 48 3e-04
UniRef50_Q9Z5R6 Cluster: Mob protein; n=5; root|Rep: Mob protein... 38 0.26
UniRef50_Q9ACP4 Cluster: Putative large secreted protein; n=1; S... 34 4.3
UniRef50_UPI00015BDEAC Cluster: UPI00015BDEAC related cluster; n... 33 7.5
UniRef50_Q38VP2 Cluster: Putative methyltransferase; n=1; Lactob... 33 7.5
UniRef50_Q4DWM3 Cluster: Putative uncharacterized protein; n=2; ... 33 9.9
>UniRef50_Q24524 Cluster: Protein singed; n=14; Endopterygota|Rep:
Protein singed - Drosophila melanogaster (Fruit fly)
Length = 512
Score = 146 bits (355), Expect = 4e-34
Identities = 70/135 (51%), Positives = 83/135 (61%)
Frame = +1
Query: 253 LDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 432
L+KYL+VD FGNV CES+E++ GS+F IS+S+D SGRWAL+N RGYFLG + DKL CTA
Sbjct: 77 LNKYLSVDQFGNVLCESDERDAGSRFQISISEDGSGRWALKNESRGYFLGGTPDKLVCTA 136
Query: 433 KVPGDAELWHVHLAARXQMNLRSIGPSVXXXXXXXXXXXXXXXXCRGARTRCSRWSSALT 612
K PG +E W VHLAAR Q+NLRSIG
Sbjct: 137 KTPGASEFWTVHLAARPQVNLRSIGRKRFAHLSESQDEIHVDANIPWGEDTLFTLEFRAE 196
Query: 613 RGGKYALHTCNNKLL 657
GG+YALHTCNNK L
Sbjct: 197 EGGRYALHTCNNKYL 211
Score = 118 bits (284), Expect = 2e-25
Identities = 53/78 (67%), Positives = 65/78 (83%)
Frame = +2
Query: 20 MNGHSNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWT 199
MNG +S GDI++QNQQKGWWTIGLIN +++Y+TAETFGFK+NANG SLKKKQ+WT
Sbjct: 1 MNGQGCELGHSNGDIISQNQQKGWWTIGLINGQHKYMTAETFGFKLNANGASLKKKQLWT 60
Query: 200 LEPASGNANDSMIYLRSH 253
LEP+ N +S+IYLRSH
Sbjct: 61 LEPS--NTGESIIYLRSH 76
Score = 77.0 bits (181), Expect = 5e-13
Identities = 33/36 (91%), Positives = 35/36 (97%)
Frame = +3
Query: 510 KRFAHLSESLDEIHVDANVPWGEDTLFTLEFRADEG 617
KRFAHLSES DEIHVDAN+PWGEDTLFTLEFRA+EG
Sbjct: 163 KRFAHLSESQDEIHVDANIPWGEDTLFTLEFRAEEG 198
>UniRef50_A7RUD3 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 488
Score = 83.4 bits (197), Expect = 5e-15
Identities = 40/88 (45%), Positives = 58/88 (65%)
Frame = +1
Query: 241 LEVTLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKL 420
L+ L +YL D GN+ CE+EE+E +KF + + D G+WAL++V YF G++ D+L
Sbjct: 54 LKSHLGRYLTADPKGNLCCEAEERESNAKFTVEIKD---GKWALKSVHGAYF-GANGDQL 109
Query: 421 TCTAKVPGDAELWHVHLAARXQMNLRSI 504
C AK+ G ELW VHLA Q+NL+S+
Sbjct: 110 KCVAKI-GKTELWTVHLAMHPQINLKSV 136
Score = 66.9 bits (156), Expect = 5e-10
Identities = 32/55 (58%), Positives = 40/55 (72%), Gaps = 1/55 (1%)
Frame = +2
Query: 92 WTIGLINSRY-RYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSH 253
W IGL+N +YLTAETFGFK+N++G SL+KKQ W+LE G A IYL+SH
Sbjct: 7 WNIGLMNDNSSKYLTAETFGFKLNSDGVSLRKKQTWSLEQVDGEA----IYLKSH 57
Score = 37.1 bits (82), Expect = 0.46
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +3
Query: 510 KRFAHLSESLDEIHVDANVPWGEDTLFTLEF 602
KR+AHL E +++ V +PWG D TL+F
Sbjct: 139 KRYAHLDEEDNDVKVSELIPWGADATITLKF 169
>UniRef50_Q6PBS5 Cluster: Fascin homolog 1-like, actin-bundling
protein; n=10; Gnathostomata|Rep: Fascin homolog 1-like,
actin-bundling protein - Danio rerio (Zebrafish)
(Brachydanio rerio)
Length = 290
Score = 74.9 bits (176), Expect = 2e-12
Identities = 39/84 (46%), Positives = 50/84 (59%)
Frame = +1
Query: 253 LDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 432
L +YLA D G V+CE+E KE +F I D GRWAL++ F G S D L+C A
Sbjct: 63 LGRYLASDKDGKVSCEAETKENDCRFLIVAQSD--GRWALQSETYLRFFGGSEDYLSCFA 120
Query: 433 KVPGDAELWHVHLAARXQMNLRSI 504
+ G+AELW +HLA Q NL S+
Sbjct: 121 QTIGEAELWAMHLALHPQANLLSV 144
Score = 67.7 bits (158), Expect = 3e-10
Identities = 32/51 (62%), Positives = 38/51 (74%)
Frame = +2
Query: 101 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSH 253
GLIN RYLTAE FGFK+NA+ SLKKKQIWTLE + + ++YLRSH
Sbjct: 14 GLINHENRYLTAEAFGFKVNASAPSLKKKQIWTLE--QDDQDGQVVYLRSH 62
Score = 41.5 bits (93), Expect = 0.021
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +3
Query: 510 KRFAHLSESLDEIHVDANVPWGEDTLFTLEF 602
KR+AHL+ EI VD N+PWG D L TL +
Sbjct: 147 KRYAHLASPEGEIAVDCNIPWGVDALLTLVY 177
>UniRef50_Q16658 Cluster: Fascin; n=49; Euteleostomi|Rep: Fascin -
Homo sapiens (Human)
Length = 493
Score = 73.7 bits (173), Expect = 4e-12
Identities = 35/84 (41%), Positives = 49/84 (58%)
Frame = +1
Query: 253 LDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 432
L +YLA D GNVTCE E P +F I DD GRW+L++ + G + D+L+C A
Sbjct: 66 LGRYLAADKDGNVTCEREVPGPDCRFLIVAHDD--GRWSLQSEAHRRYFGGTEDRLSCFA 123
Query: 433 KVPGDAELWHVHLAARXQMNLRSI 504
+ AE W VH+A Q+N+ S+
Sbjct: 124 QTVSPAEKWSVHIAMHPQVNIYSV 147
Score = 68.1 bits (159), Expect = 2e-10
Identities = 31/51 (60%), Positives = 37/51 (72%)
Frame = +2
Query: 101 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRSH 253
GLIN +YLTAE FGFK+NA+ +SLKKKQIWTLE A + + LRSH
Sbjct: 15 GLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSH 65
Score = 42.3 bits (95), Expect = 0.012
Identities = 20/33 (60%), Positives = 24/33 (72%), Gaps = 1/33 (3%)
Frame = +3
Query: 510 KRFAHLS-ESLDEIHVDANVPWGEDTLFTLEFR 605
KR+AHLS DEI VD +VPWG D+L TL F+
Sbjct: 150 KRYAHLSARPADEIAVDRDVPWGVDSLITLAFQ 182
>UniRef50_Q4RZ67 Cluster: Chromosome undetermined SCAF14961, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF14961,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 325
Score = 72.9 bits (171), Expect = 8e-12
Identities = 34/84 (40%), Positives = 52/84 (61%)
Frame = +1
Query: 253 LDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 432
L +Y+A D GNVT ++E P S+F I+ DD GRW+L++ G +LG + D++ C A
Sbjct: 70 LGRYIAADKDGNVTGDAESPGPESRFLITAHDD--GRWSLQSEAHGRYLGGTEDRIICFA 127
Query: 433 KVPGDAELWHVHLAARXQMNLRSI 504
+ AE W VH+A Q+N+ S+
Sbjct: 128 QSASVAEKWSVHIAMHPQVNIFSV 151
Score = 71.7 bits (168), Expect = 2e-11
Identities = 39/74 (52%), Positives = 50/74 (67%)
Frame = +2
Query: 32 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPA 211
+NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQIWTLE +
Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQIWTLEQS 55
Query: 212 SGNANDSMIYLRSH 253
+A+ ++ LRSH
Sbjct: 56 GDDASGNLFCLRSH 69
>UniRef50_UPI0000E5925D Cluster: fascin 2 isoform 2; n=1; Homo
sapiens|Rep: fascin 2 isoform 2 - Homo sapiens
Length = 516
Score = 68.5 bits (160), Expect = 2e-10
Identities = 34/84 (40%), Positives = 45/84 (53%)
Frame = +1
Query: 253 LDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTA 432
L +YL+ + G V CE+E+ PG V GRW LR+ G F G + D+L+C A
Sbjct: 65 LGRYLSAEEDGRVACEAEQ--PGRDCRFLVLPQPDGRWVLRSEPHGRFFGGTEDQLSCFA 122
Query: 433 KVPGDAELWHVHLAARXQMNLRSI 504
AELW VHLA Q +L S+
Sbjct: 123 TAVSPAELWTVHLAIHPQAHLLSV 146
Score = 59.7 bits (138), Expect = 8e-08
Identities = 28/50 (56%), Positives = 35/50 (70%)
Frame = +2
Query: 101 GLINSRYRYLTAETFGFKINANGTSLKKKQIWTLEPASGNANDSMIYLRS 250
GL+N RYLTAE+FGFK+NA+ SLK+KQ W LEP G + + LRS
Sbjct: 15 GLVNDTDRYLTAESFGFKVNASAPSLKRKQTWVLEPDPGQG--TAVLLRS 62
Score = 36.7 bits (81), Expect = 0.61
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +3
Query: 510 KRFAHLSESLDEIHVDANVPWGEDTLFTLEFRA 608
+R+ HL DE+ D + PWG D L TL FR+
Sbjct: 149 RRYVHLCPREDEMAADGDKPWGVDALLTLIFRS 181
>UniRef50_Q4RZ66 Cluster: Chromosome undetermined SCAF14961, whole
genome shotgun sequence; n=1; Tetraodon
nigroviridis|Rep: Chromosome undetermined SCAF14961,
whole genome shotgun sequence - Tetraodon nigroviridis
(Green puffer)
Length = 353
Score = 61.3 bits (142), Expect = 2e-08
Identities = 34/58 (58%), Positives = 40/58 (68%)
Frame = +2
Query: 32 SNGDINSAGDILTQNQQKGWWTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLE 205
+NG N D+L Q Q GLIN +YLTAETFGFKINA+ +S+KKKQIWTLE
Sbjct: 3 TNGASNGTSDML-QIQ------FGLINCGNKYLTAETFGFKINASASSMKKKQIWTLE 53
>UniRef50_Q05634 Cluster: Fascin; n=1; Strongylocentrotus
purpuratus|Rep: Fascin - Strongylocentrotus purpuratus
(Purple sea urchin)
Length = 496
Score = 52.8 bits (121), Expect = 9e-06
Identities = 22/38 (57%), Positives = 29/38 (76%)
Frame = +2
Query: 92 WTIGLINSRYRYLTAETFGFKINANGTSLKKKQIWTLE 205
+ GL+NS RYLTAE FG K+NA+G +LK +Q+W LE
Sbjct: 8 YKFGLVNSAGRYLTAEKFGGKVNASGATLKARQVWILE 45
Score = 46.4 bits (105), Expect = 7e-04
Identities = 22/89 (24%), Positives = 43/89 (48%), Gaps = 1/89 (1%)
Frame = +1
Query: 241 LEVTLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKL 420
L+ +L+ D GNV C E++ + + G+WAL+NV +L + ++L
Sbjct: 55 LKAPSGNFLSADKNGNVYCSVEDRTEDADTGFEIELQPDGKWALKNVSHQRYLACNGEEL 114
Query: 421 TCT-AKVPGDAELWHVHLAARXQMNLRSI 504
C+ + + W V LA Q+ ++++
Sbjct: 115 ICSESSTSNPSANWTVQLAIHPQVCMKNV 143
>UniRef50_UPI00005846A8 Cluster: PREDICTED: hypothetical protein;
n=1; Strongylocentrotus purpuratus|Rep: PREDICTED:
hypothetical protein - Strongylocentrotus purpuratus
Length = 635
Score = 49.6 bits (113), Expect = 8e-05
Identities = 23/82 (28%), Positives = 40/82 (48%), Gaps = 1/82 (1%)
Frame = +1
Query: 262 YLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTAKVP 441
YL+VD G VT ++EE +F + +G W +R+V ++G S K+ C +
Sbjct: 70 YLSVDKSGKVTADAEEIGEEQRFEVDAGSGGNGAWVIRSVAHDTYMGIKSKKVLCKMRES 129
Query: 442 GDA-ELWHVHLAARXQMNLRSI 504
+ + W+V L+ L S+
Sbjct: 130 SSSDQRWYVRLSIHPHCCLLSV 151
Score = 33.1 bits (72), Expect = 7.5
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 504 RPKRFAHLSESLDEIHVDANVPWGEDTLFTLEFRADEG 617
+ ++FAH+ +S + DA VPW DTL L++ G
Sbjct: 152 KAEKFAHVPQSGRYLTFDATVPWRSDTLLQLKYSEGYG 189
>UniRef50_Q5C7Y0 Cluster: SJCHGC09066 protein; n=1; Schistosoma
japonicum|Rep: SJCHGC09066 protein - Schistosoma
japonicum (Blood fluke)
Length = 248
Score = 47.6 bits (108), Expect = 3e-04
Identities = 22/86 (25%), Positives = 48/86 (55%)
Frame = +1
Query: 241 LEVTLDKYLAVDSFGNVTCESEEKEPGSKFHISVSDDNSGRWALRNVERGYFLGSSSDKL 420
L+ +L ++L+V+ G ++ S + + +F + + D +G WA ++ G++L S ++
Sbjct: 63 LQNSLGRFLSVNKDGKISA-SLKSDSEERFRLEFAPDGTGEWAFKSDTYGFYLSGSDTQV 121
Query: 421 TCTAKVPGDAELWHVHLAARXQMNLR 498
+C +K P W + LA Q+++R
Sbjct: 122 SCFSKSP---VWWSIRLATHPQVHIR 144
Score = 40.3 bits (90), Expect = 0.049
Identities = 15/27 (55%), Positives = 23/27 (85%)
Frame = +2
Query: 122 RYLTAETFGFKINANGTSLKKKQIWTL 202
R+LTAE F +IN +GT+L+++Q+WTL
Sbjct: 25 RFLTAEVFNNEINISGTALRRRQVWTL 51
>UniRef50_Q9Z5R6 Cluster: Mob protein; n=5; root|Rep: Mob protein -
Bordetella bronchiseptica (Alcaligenes bronchisepticus)
Length = 329
Score = 37.9 bits (84), Expect = 0.26
Identities = 23/60 (38%), Positives = 30/60 (50%)
Frame = -3
Query: 674 SPXAVSSNLLLQVWSAYLPPLVSAELQREQRVLAPRHVSVHVDLVERLREVSETLGPIER 495
+P AV+ L V Y P L +A RE R A + DL ERL+ V + LGP+ R
Sbjct: 225 TPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVLDALGPLNR 284
>UniRef50_Q9ACP4 Cluster: Putative large secreted protein; n=1;
Streptomyces coelicolor|Rep: Putative large secreted
protein - Streptomyces coelicolor
Length = 2082
Score = 33.9 bits (74), Expect = 4.3
Identities = 19/51 (37%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = +1
Query: 319 GSKFHISVSDDNSGRWALRNVERGYFLGSSSDKLTCTAKVPG--DAELWHV 465
G + ++ V DD+SG+W L + + G+ +KLT TA+ G D E W V
Sbjct: 367 GGQSNVLVKDDDSGQWHLEDDD-----GTKVEKLTDTARANGDNDGEYWRV 412
>UniRef50_UPI00015BDEAC Cluster: UPI00015BDEAC related cluster; n=1;
unknown|Rep: UPI00015BDEAC UniRef100 entry - unknown
Length = 190
Score = 33.1 bits (72), Expect = 7.5
Identities = 19/54 (35%), Positives = 28/54 (51%)
Frame = +1
Query: 178 KEETDLDPRACFRECQ**HDLLEVTLDKYLAVDSFGNVTCESEEKEPGSKFHIS 339
K T+L A REC ++E+ L KY+ V+S G+V S + +KF S
Sbjct: 91 KTLTELFKDALIRECH--VKIIEINLSKYVTVNSHGDVALSSNVNDLRTKFRTS 142
>UniRef50_Q38VP2 Cluster: Putative methyltransferase; n=1;
Lactobacillus sakei subsp. sakei 23K|Rep: Putative
methyltransferase - Lactobacillus sakei subsp. sakei
(strain 23K)
Length = 186
Score = 33.1 bits (72), Expect = 7.5
Identities = 19/44 (43%), Positives = 27/44 (61%), Gaps = 4/44 (9%)
Frame = +3
Query: 498 LDRPKRFAH--LSESL--DEIHVDANVPWGEDTLFTLEFRADEG 617
LD+P FAH L +++ ++ +DA V G+DTLF E AD G
Sbjct: 3 LDQPSSFAHFLLDQAIRPGDVVIDATVGHGDDTLFLAEKVADYG 46
>UniRef50_Q4DWM3 Cluster: Putative uncharacterized protein; n=2;
Trypanosoma cruzi|Rep: Putative uncharacterized protein
- Trypanosoma cruzi
Length = 517
Score = 32.7 bits (71), Expect = 9.9
Identities = 17/37 (45%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = +3
Query: 492 PPLDRPKRFAHLSES-LDEIHVDANVPWGEDTLFTLE 599
P L P R HL+E D + V ANVPW E F E
Sbjct: 448 PQLSIPGRMLHLTEEGTDVLDVPANVPWREQLFFGKE 484
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 650,540,492
Number of Sequences: 1657284
Number of extensions: 11874471
Number of successful extensions: 37756
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 36250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37743
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 61323318355
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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