Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30688.Seq
(648 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces pom... 27 2.3
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 26 5.4
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 25 7.1
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 25 9.4
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 9.4
SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit ... 25 9.4
SPAC57A10.12c |ura3||dihydroorotate dehydrogenase Ura3|Schizosac... 25 9.4
SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 25 9.4
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 9.4
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 25 9.4
>SPAC22H10.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 158
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -2
Query: 470 GIPVIRPLMMESTALGAAIVAGRAMRVW 387
GIP+I P+ L A+ + RA ++W
Sbjct: 131 GIPIIDPVTRAPAVLAGAVSSSRAKQMW 158
>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 897
Score = 25.8 bits (54), Expect = 5.4
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = -2
Query: 275 WTDTKNEMSTQKNQIELLQFCRRDFFSSEPPY 180
W +T+N + N +E+L R+ S P Y
Sbjct: 764 WANTENARYSTSNALEILDMLLREKIESAPRY 795
>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 488
Score = 25.4 bits (53), Expect = 7.1
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -2
Query: 374 PSPPADTFLPALTNXXXXXXXXXXXEALNKCMGWTDTKNEMST 246
P PP+ T LPAL + E T+TK E+ST
Sbjct: 61 PRPPSATSLPALLSLFQEEWDSVALEQFELRRNLTETKQELST 103
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 25.0 bits (52), Expect = 9.4
Identities = 12/53 (22%), Positives = 25/53 (47%)
Frame = +1
Query: 370 LGIVVGHTRIARPATMAAPRAVLSIMRGRMTGIPSKSACICIRTEFSPSLRRR 528
LG +G + P +++P + + IP + + + +FSP+L +R
Sbjct: 206 LGFKMGRLKTGTPPRLSSPINISKMTEQTGDEIPETFSFLNLERDFSPALPQR 258
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.0 bits (52), Expect = 9.4
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +3
Query: 348 QECIGRWAGYCRGPHTHCATC 410
QEC+ W G+ + THC C
Sbjct: 36 QECLVEWLGHSK--KTHCELC 54
>SPBC36B7.03 |sec63||ER protein translocation subcomplex subunit
Sec63 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/63 (23%), Positives = 30/63 (47%)
Frame = -2
Query: 527 RRRRDGENSVLMQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPTTIPSPPADTFL 348
R++ + N+ + Q+D+ V+ L+ +TA G A +++W + + P D
Sbjct: 281 RKKTNNFNTHQILSQSDV----VLNALLSVATAFGFANPVDNVLKLWQHIVQAIPLDAPF 336
Query: 347 PAL 339
P L
Sbjct: 337 PLL 339
>SPAC57A10.12c |ura3||dihydroorotate dehydrogenase
Ura3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 443
Score = 25.0 bits (52), Expect = 9.4
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 7/72 (9%)
Frame = +1
Query: 373 GIVVGHTRIARPATMAAPRAVLSIMRGRMTGIPSKSACI----CIRTEFS---PSLRRRG 531
G++VG+T + RP T+ + V G ++G P K + +R S P + G
Sbjct: 330 GVIVGNTTVQRPKTLKSTSHVEE--TGGLSGPPLKPIALNTLRTLRKHLSSDIPIIGCGG 387
Query: 532 VASGARSLQPSR 567
++SG +++ +R
Sbjct: 388 ISSGKDAIEYAR 399
>SPAC19A8.12 |dcp2||mRNA decapping complex subunit
Dcp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 25.0 bits (52), Expect = 9.4
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%)
Frame = +3
Query: 273 PSHAFIQGFFPTNSPHFSFF-IGKSRQECIGRWAGYCRGPHT 395
PS + Q F+P S S + +GK+ Q G + Y G T
Sbjct: 410 PSTVYHQVFYPPTSTSVSSYGLGKTPQPAYGSSSPYVNGHQT 451
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 25.0 bits (52), Expect = 9.4
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +2
Query: 371 WVLSWATHALRDLRQWPPPGLYF 439
W L+ A D+R WPP ++F
Sbjct: 403 WTLTDLADAFLDVRLWPPIFMFF 425
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 25.0 bits (52), Expect = 9.4
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +3
Query: 228 FYLILLR*HFIFSIGPSHAFIQGFFPTNSPHFSFFIGKSRQECIGRWAGY 377
FYL + H I +IGP I +F ++ H +G + + G GY
Sbjct: 358 FYLFIDEAHSIGAIGPRGGGICDYFGISTDHVDILMG-TFTKSFGAAGGY 406
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,618,683
Number of Sequences: 5004
Number of extensions: 55302
Number of successful extensions: 155
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 291768710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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