Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30687.Seq
         (698 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc...    97   2e-21
SPBC13G1.02 |||mannose-1-phosphate guanyltransferase |Schizosacc...    44   2e-05
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon...    31   0.16 
SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces p...    25   7.9  
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce...    25   7.9  
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb...    25   7.9  

>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
           Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 363

 Score = 97.5 bits (232), Expect = 2e-21
 Identities = 45/65 (69%), Positives = 53/65 (81%), Gaps = 2/65 (3%)
 Frame = +3

Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKVEEPSKYGVVVYDDNGQ--IESFIEKPQEFISN 683
           VICE+PF +LA +HK HG EGTIVVTKVEEPSKYGVVV+  N +  IE F+EKP EF+SN
Sbjct: 111 VICEYPFADLAAFHKAHGAEGTIVVTKVEEPSKYGVVVHYPNSESLIERFVEKPVEFVSN 170

Query: 684 QINAG 698
           +IN G
Sbjct: 171 RINGG 175



 Score = 94.3 bits (224), Expect = 2e-20
 Identities = 43/78 (55%), Positives = 58/78 (74%)
 Frame = +1

Query: 256 VEFANKPILMHQIEALVEAGVTQVILAVSYRAEEMEKELTEQVSKLGVSLTFSHETEPLG 435
           VEF NKP+++HQ+EAL  AGVT ++LAV+YR E M + L +   +  V++TFS E EPLG
Sbjct: 26  VEFGNKPMILHQVEALAAAGVTDIVLAVNYRPEIMVEALKKYEKEYNVNITFSVENEPLG 85

Query: 436 TAGPLALARELLSTSSEP 489
           TAGPLALAR++L+    P
Sbjct: 86  TAGPLALARDILAKDHSP 103



 Score = 44.8 bits (101), Expect = 1e-05
 Identities = 19/24 (79%), Positives = 23/24 (95%)
 Frame = +2

Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253
           ++ALILVGG+GTRLRPLTL+ PKP
Sbjct: 1   MKALILVGGFGTRLRPLTLTLPKP 24


>SPBC13G1.02 |||mannose-1-phosphate guanyltransferase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 414

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 3/66 (4%)
 Frame = +3

Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYD-DNGQIESFIEKPQEFIS 680
           V C FP +EL   H       T++ TKV  E+ S +G +V +   G++  +++KP  ++S
Sbjct: 119 VCCSFPLQELLNVHHEKKALVTLMATKVSKEDASNFGCLVEEPSTGRVLHYVDKPSSYLS 178

Query: 681 NQINAG 698
           N I+ G
Sbjct: 179 NIISCG 184



 Score = 29.5 bits (63), Expect = 0.48
 Identities = 15/24 (62%), Positives = 17/24 (70%), Gaps = 2/24 (8%)
 Frame = +2

Query: 188 ALILVGG--YGTRLRPLTLSRPKP 253
           A+ILVGG   GTR RPL+   PKP
Sbjct: 5   AVILVGGPSRGTRFRPLSFDVPKP 28


>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 678

 Score = 31.1 bits (67), Expect = 0.16
 Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 3/38 (7%)
 Frame = +2

Query: 164 DKKLEEIRALILVGGYGTRLRPLTLSRPK---PRSNSP 268
           +K    ++A++L   Y  R RPLTL +P+   P +N+P
Sbjct: 12  EKPKHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTP 49


>SPAC11E3.05 |||ubiquitin-protein ligase E3|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1323

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/36 (36%), Positives = 15/36 (41%), Gaps = 1/36 (2%)
 Frame = -3

Query: 519  HKLHRVQYKEWFRTGAEQFPGKCQWSC-CSQWFSLM 415
            H +H   YKEWF  G +     C   C C   F  M
Sbjct: 1284 HVMHEDCYKEWFSNG-DSISQSCSSGCGCKCQFQHM 1318


>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 11/22 (50%), Positives = 16/22 (72%)
 Frame = -2

Query: 541 ASSLNGNSQITQSSIQRMVPNW 476
           AS +  +S+  +SSIQR +PNW
Sbjct: 794 ASIILLDSRYNRSSIQRKLPNW 815


>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 2386

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/24 (41%), Positives = 16/24 (66%)
 Frame = +2

Query: 2    SKQNLCLRTYIHTPLETNLAECAY 73
            +++NLC+RTY+  PL     EC +
Sbjct: 2112 NRRNLCIRTYVVIPLN---EECGF 2132


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,668,621
Number of Sequences: 5004
Number of extensions: 50581
Number of successful extensions: 130
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)