Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30687.Seq
(698 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1) i... 90 1e-18
At4g30570.1 68417.m04338 GDP-mannose pyrophosphorylase, putative... 87 8e-18
At3g55590.1 68416.m06173 GDP-mannose pyrophosphorylase, putative... 85 6e-17
At1g74910.3 68414.m08687 ADP-glucose pyrophosphorylase family pr... 50 1e-06
At1g74910.2 68414.m08686 ADP-glucose pyrophosphorylase family pr... 50 1e-06
At1g74910.1 68414.m08685 ADP-glucose pyrophosphorylase family pr... 50 1e-06
At2g04650.1 68415.m00474 ADP-glucose pyrophosphorylase family pr... 48 5e-06
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 31 0.56
At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase... 31 0.97
At5g19220.1 68418.m02289 glucose-1-phosphate adenylyltransferase... 31 0.97
At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 29 2.2
At4g39210.1 68417.m05551 glucose-1-phosphate adenylyltransferase... 28 5.2
At3g45290.1 68416.m04890 seven transmembrane MLO family protein ... 28 5.2
At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 5.2
At1g14440.2 68414.m01713 zinc finger homeobox family protein / Z... 28 6.8
At1g14440.1 68414.m01712 zinc finger homeobox family protein / Z... 28 6.8
At5g24480.1 68418.m02885 hypothetical protein similar to unknown... 27 9.0
At1g51310.1 68414.m05770 tRNA methyl transferase family protein ... 27 9.0
>At2g39770.1 68415.m04883 GDP-mannose pyrophosphorylase (GMP1)
identical to GDP-mannose pyrophosphorylase from
Arabidopsis thaliana [GI:3598958]; updated per Conklin
PL et al, PNAS 1999, 96(7):4198-203
Length = 361
Score = 89.8 bits (213), Expect = 1e-18
Identities = 45/79 (56%), Positives = 59/79 (74%), Gaps = 1/79 (1%)
Frame = +1
Query: 256 VEFANKPILMHQIEALVEAGVTQVILAVSYRAEEMEKELTEQVSKLGVSLTFSHETEPLG 435
V+FANKP+++HQIEAL GV +V+LA++Y+ E M L + +KL + +T S ETEPLG
Sbjct: 26 VDFANKPMILHQIEALKAVGVDEVVLAINYQPEVMLNFLKDFETKLEIKITCSQETEPLG 85
Query: 436 TAGPLALARE-LLSTSSEP 489
TAGPLALAR+ LL S EP
Sbjct: 86 TAGPLALARDKLLDGSGEP 104
Score = 86.2 bits (204), Expect = 2e-17
Identities = 36/64 (56%), Positives = 53/64 (82%), Gaps = 1/64 (1%)
Frame = +3
Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKVEEPSKYGVVVYDDN-GQIESFIEKPQEFISNQ 686
VI E+P KE+ +HK+HG E +I+VTKV+EPSKYGVVV +++ G++E F+EKP+ ++ N+
Sbjct: 112 VISEYPLKEMLEFHKSHGGEASIMVTKVDEPSKYGVVVMEESTGRVEKFVEKPKLYVGNK 171
Query: 687 INAG 698
INAG
Sbjct: 172 INAG 175
Score = 45.6 bits (103), Expect = 3e-05
Identities = 20/24 (83%), Positives = 23/24 (95%)
Frame = +2
Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253
++ALILVGG+GTRLRPLTLS PKP
Sbjct: 1 MKALILVGGFGTRLRPLTLSFPKP 24
>At4g30570.1 68417.m04338 GDP-mannose pyrophosphorylase, putative
similar to GDP-mannose pyrophosphorylase [Arabidopsis
thaliana] GI:3598958; contains Pfam profile PF00483:
Nucleotidyl transferase
Length = 331
Score = 87.4 bits (207), Expect = 8e-18
Identities = 44/80 (55%), Positives = 60/80 (75%), Gaps = 2/80 (2%)
Frame = +1
Query: 256 VEFANKPILMHQIEALVEAGVTQVILAVSYRAEE-MEKELTEQVSKLGVSLTFSHETEPL 432
V+F NKP+++HQIEAL AGVT+V+LA++++ E M + E KL + +TFS ETEPL
Sbjct: 6 VDFGNKPMILHQIEALKGAGVTEVVLAINHQQPEVMLNFVKEYEKKLEIKITFSQETEPL 65
Query: 433 GTAGPLALARE-LLSTSSEP 489
GTAGPLALAR+ L+ S +P
Sbjct: 66 GTAGPLALARDKLVDESGQP 85
Score = 79.4 bits (187), Expect = 2e-15
Identities = 33/64 (51%), Positives = 50/64 (78%), Gaps = 1/64 (1%)
Frame = +3
Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKVEEPSKYGVVVYDD-NGQIESFIEKPQEFISNQ 686
VICE+P E+ +HK + E +I+VT+V++PSKYGVVV ++ ++ESF+EKP+ F+ N+
Sbjct: 93 VICEYPLLEMIEFHKTNRAEASIMVTEVDDPSKYGVVVTEEGTARVESFVEKPKHFVGNK 152
Query: 687 INAG 698
INAG
Sbjct: 153 INAG 156
>At3g55590.1 68416.m06173 GDP-mannose pyrophosphorylase, putative
strong similarity to GDP-mannose pyrophosphorylase from
Arabidopsis thaliana [GI:3598958], Pichia angusta
[GI:7331158]; contains Pfam profile PF00483 Nucleotidyl
transferase
Length = 364
Score = 84.6 bits (200), Expect = 6e-17
Identities = 43/82 (52%), Positives = 59/82 (71%), Gaps = 4/82 (4%)
Frame = +1
Query: 256 VEFANKPILMHQIEALVEAGVTQVILAVSYRAEE---MEKELTEQVSKLGVSLTFSHETE 426
V+FANKP+++HQIEAL GV +V+LA++Y E+ M K + + LG+ +T S ETE
Sbjct: 26 VDFANKPMILHQIEALKAIGVDEVVLAINYEPEQLLVMSKFSNDVEATLGIKITCSQETE 85
Query: 427 PLGTAGPLALARE-LLSTSSEP 489
PLGTAGPLALAR+ L+ S +P
Sbjct: 86 PLGTAGPLALARDKLVDGSGQP 107
Score = 80.2 bits (189), Expect = 1e-15
Identities = 34/64 (53%), Positives = 50/64 (78%), Gaps = 1/64 (1%)
Frame = +3
Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKVEEPSKYGVVVYDD-NGQIESFIEKPQEFISNQ 686
VI ++P +E+ +H HG E +I+VTKV+EPSKYGVVV ++ G++E F+EKP+ F+ N+
Sbjct: 115 VISDYPLEEMIAFHNAHGGEASIMVTKVDEPSKYGVVVMEEATGRVERFVEKPKLFVGNK 174
Query: 687 INAG 698
INAG
Sbjct: 175 INAG 178
Score = 46.0 bits (104), Expect = 2e-05
Identities = 20/24 (83%), Positives = 23/24 (95%)
Frame = +2
Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253
++ALILVGG+GTRLRPLTLS PKP
Sbjct: 1 MKALILVGGFGTRLRPLTLSLPKP 24
>At1g74910.3 68414.m08687 ADP-glucose pyrophosphorylase family
protein contains Pfam profile PF00483: Nucleotidyl
transferase; low similarity to mannose-1-phosphate
guanylyltransferase [Hypocrea jecorina] GI:3323397
Length = 387
Score = 50.4 bits (115), Expect = 1e-06
Identities = 25/73 (34%), Positives = 42/73 (57%), Gaps = 3/73 (4%)
Frame = +3
Query: 489 ILCIELCVICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYDD-NGQIESFIE 659
I + V C FP ++ H+ +G GT++V KV E S++G +V D ++ + E
Sbjct: 116 IFLLNCDVCCSFPLPKMLEAHRGYGGIGTLLVIKVSPESASQFGELVADPVTNELLHYTE 175
Query: 660 KPQEFISNQINAG 698
KP+ F+S++IN G
Sbjct: 176 KPETFVSDRINCG 188
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/28 (57%), Positives = 22/28 (78%), Gaps = 2/28 (7%)
Frame = +2
Query: 176 EEIRALILVGG--YGTRLRPLTLSRPKP 253
E++ A+I+VGG GTR RPL+L+ PKP
Sbjct: 7 EKVVAVIMVGGPTKGTRFRPLSLNIPKP 34
>At1g74910.2 68414.m08686 ADP-glucose pyrophosphorylase family
protein contains Pfam profile PF00483: Nucleotidyl
transferase; low similarity to mannose-1-phosphate
guanylyltransferase [Hypocrea jecorina] GI:3323397
Length = 415
Score = 50.4 bits (115), Expect = 1e-06
Identities = 25/73 (34%), Positives = 42/73 (57%), Gaps = 3/73 (4%)
Frame = +3
Query: 489 ILCIELCVICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYDD-NGQIESFIE 659
I + V C FP ++ H+ +G GT++V KV E S++G +V D ++ + E
Sbjct: 116 IFLLNCDVCCSFPLPKMLEAHRGYGGIGTLLVIKVSPESASQFGELVADPVTNELLHYTE 175
Query: 660 KPQEFISNQINAG 698
KP+ F+S++IN G
Sbjct: 176 KPETFVSDRINCG 188
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/28 (57%), Positives = 22/28 (78%), Gaps = 2/28 (7%)
Frame = +2
Query: 176 EEIRALILVGG--YGTRLRPLTLSRPKP 253
E++ A+I+VGG GTR RPL+L+ PKP
Sbjct: 7 EKVVAVIMVGGPTKGTRFRPLSLNIPKP 34
>At1g74910.1 68414.m08685 ADP-glucose pyrophosphorylase family
protein contains Pfam profile PF00483: Nucleotidyl
transferase; low similarity to mannose-1-phosphate
guanylyltransferase [Hypocrea jecorina] GI:3323397
Length = 415
Score = 50.4 bits (115), Expect = 1e-06
Identities = 25/73 (34%), Positives = 42/73 (57%), Gaps = 3/73 (4%)
Frame = +3
Query: 489 ILCIELCVICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYDD-NGQIESFIE 659
I + V C FP ++ H+ +G GT++V KV E S++G +V D ++ + E
Sbjct: 116 IFLLNCDVCCSFPLPKMLEAHRGYGGIGTLLVIKVSPESASQFGELVADPVTNELLHYTE 175
Query: 660 KPQEFISNQINAG 698
KP+ F+S++IN G
Sbjct: 176 KPETFVSDRINCG 188
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/28 (57%), Positives = 22/28 (78%), Gaps = 2/28 (7%)
Frame = +2
Query: 176 EEIRALILVGG--YGTRLRPLTLSRPKP 253
E++ A+I+VGG GTR RPL+L+ PKP
Sbjct: 7 EKVVAVIMVGGPTKGTRFRPLSLNIPKP 34
>At2g04650.1 68415.m00474 ADP-glucose pyrophosphorylase family
protein contains Pfam profile PF00483: Nucleotidyl
transferase; low similarity to mannose-1-phosphate
guanylyltransferase [Hypocrea jecorina] GI:3323397
Length = 406
Score = 48.4 bits (110), Expect = 5e-06
Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 3/66 (4%)
Frame = +3
Query: 510 VICEFPFKELARYHKNHGKEGTIVVTKV--EEPSKYGVVVYD-DNGQIESFIEKPQEFIS 680
V C FP + + H+ +G GT++V KV E S++G ++ D D ++ + EKP+ F+S
Sbjct: 120 VCCSFPLQGILDAHRRYGGIGTMLVIKVSAEAASQFGELIADPDTKELLHYTEKPETFVS 179
Query: 681 NQINAG 698
+ IN G
Sbjct: 180 DLINCG 185
Score = 32.3 bits (70), Expect = 0.32
Identities = 15/28 (53%), Positives = 21/28 (75%), Gaps = 2/28 (7%)
Frame = +2
Query: 176 EEIRALILVGG--YGTRLRPLTLSRPKP 253
E++ A+I+VGG GTR RPL+ + PKP
Sbjct: 4 EKVVAVIMVGGPTKGTRFRPLSFNTPKP 31
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 31.5 bits (68), Expect = 0.56
Identities = 14/26 (53%), Positives = 18/26 (69%)
Frame = +2
Query: 176 EEIRALILVGGYGTRLRPLTLSRPKP 253
+ + ++IL GG GTRL PLT R KP
Sbjct: 83 KNVASIILGGGAGTRLFPLTSKRAKP 108
>At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase
small subunit 1 (APS1) / ADP-glucose pyrophosphorylase
(ADG1) identical to SP|P55228
Length = 520
Score = 30.7 bits (66), Expect = 0.97
Identities = 14/24 (58%), Positives = 16/24 (66%)
Frame = +2
Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253
+ +IL GG GTRL PLT R KP
Sbjct: 90 VLGIILGGGAGTRLYPLTKKRAKP 113
>At5g19220.1 68418.m02289 glucose-1-phosphate adenylyltransferase
large subunit 1 (APL1) / ADP-glucose pyrophosphorylase
(ADG2) identical to SP|P55229
Length = 522
Score = 30.7 bits (66), Expect = 0.97
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +2
Query: 182 IRALILVGGYGTRLRPLTLSRPKP 253
+ ++IL GG GTRL PLT R KP
Sbjct: 90 VASIILGGGAGTRLFPLTKRRAKP 113
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/49 (26%), Positives = 28/49 (57%), Gaps = 2/49 (4%)
Frame = +3
Query: 528 FKELARYHKNHGKEGTIVVTKVEE--PSKYGVVVYDDNGQIESFIEKPQ 668
+ + + H+ G + +I +++ S +G++ DD G++ SF EKP+
Sbjct: 225 YMDFIQDHRQSGADISISCIPIDDRRASDFGLMKIDDKGRVISFSEKPK 273
>At2g34970.1 68415.m04291 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q64350
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Rattus norvegicus};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 730
Score = 29.5 bits (63), Expect = 2.2
Identities = 10/23 (43%), Positives = 17/23 (73%)
Frame = +2
Query: 182 IRALILVGGYGTRLRPLTLSRPK 250
++A++L + T+ RP+TL RPK
Sbjct: 25 LQAILLADSFATKFRPVTLERPK 47
>At4g39210.1 68417.m05551 glucose-1-phosphate adenylyltransferase
large subunit 3 (APL3) / ADP-glucose pyrophosphorylase
identical to SP|P55231
Length = 521
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +3
Query: 528 FKELARYHKNHGKEGTIVVTKVEEP--SKYGVVVYDDNGQIESFIEKP 665
+ + ++H + + T+ V+E S+YG+V D +G++ F EKP
Sbjct: 224 YMDFVQHHVDSKADITLSCAPVDESRASEYGLVNIDRSGRVVHFSEKP 271
>At3g45290.1 68416.m04890 seven transmembrane MLO family protein /
MLO-like protein 3 (MLO3) membrane protein Mlo3
[Arabidopsis thaliana] gi|14091576|gb|AAK53796; similar
to MLO protein SWISS-PROT:P93766, NCBI_gi:1877221
[Hordeum vulgare][Barley]
Length = 508
Score = 28.3 bits (60), Expect = 5.2
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -1
Query: 644 YLTIIIINNHTIFGWLFYFCNHYCTFLPMILVI 546
+LT++I + GW YF Y +F+P+I+V+
Sbjct: 285 WLTVVIFMLLDVSGWRVYF---YMSFVPLIIVL 314
>At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon
domain-containing protein similar to SP|Q13144
Translation initiation factor eIF-2B epsilon subunit
(eIF-2B GDP-GTP exchange factor) {Homo sapiens};
contains Pfam profile PF02020:
eIF4-gamma/eIF5/eIF2-epsilon
Length = 676
Score = 28.3 bits (60), Expect = 5.2
Identities = 10/24 (41%), Positives = 17/24 (70%)
Frame = +2
Query: 176 EEIRALILVGGYGTRLRPLTLSRP 247
+ ++A++L + T+L PLTL RP
Sbjct: 23 QRLQAILLADSFATKLLPLTLERP 46
>At1g14440.2 68414.m01713 zinc finger homeobox family protein /
ZF-HD homeobox family protein
Length = 312
Score = 27.9 bits (59), Expect = 6.8
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = -3
Query: 639 DHYHHKQPHHI 607
DH+HH+ PHH+
Sbjct: 67 DHHHHQDPHHV 77
>At1g14440.1 68414.m01712 zinc finger homeobox family protein /
ZF-HD homeobox family protein
Length = 312
Score = 27.9 bits (59), Expect = 6.8
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = -3
Query: 639 DHYHHKQPHHI 607
DH+HH+ PHH+
Sbjct: 67 DHHHHQDPHHV 77
>At5g24480.1 68418.m02885 hypothetical protein similar to unknown
protein (gb|AAD32930.1)
Length = 350
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +2
Query: 221 LRPLTLSRPKPRSNSPTNL 277
L PLT SRP P+SN P+ L
Sbjct: 129 LSPLTNSRPIPKSNDPSPL 147
>At1g51310.1 68414.m05770 tRNA methyl transferase family protein
contains Pfam PF03054: tRNA methyl transferase profile;
similar to tRNA
(5-methylaminomethyl-2-thiouridylate)-methyltransferase
(EC 2.1.1.61). (Swiss-Prot:Q8ZPZ4) [Salmonella
typhimurium]
Length = 421
Score = 27.5 bits (58), Expect = 9.0
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -3
Query: 558 DSCDILQVL*METHKLHRVQYKEWFRTGAEQFPGKCQW 445
DS L++L H K WF+ G E F +C W
Sbjct: 17 DSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPW 54
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,828,472
Number of Sequences: 28952
Number of extensions: 263424
Number of successful extensions: 667
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 657
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -