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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30684.Seq
         (756 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g22270.1 68414.m02783 expressed protein contains Pfam domain ...    61   7e-10
At1g78190.1 68414.m09111 expressed protein                             58   6e-09
At2g37840.2 68415.m04646 protein kinase family protein contains ...    28   7.7  
At2g37840.1 68415.m04645 protein kinase family protein contains ...    28   7.7  

>At1g22270.1 68414.m02783 expressed protein contains Pfam domain
           PF03966: Protein of unknown function (DUF343)
          Length = 124

 Score = 61.3 bits (142), Expect = 7e-10
 Identities = 33/92 (35%), Positives = 48/92 (52%), Gaps = 2/92 (2%)
 Frame = +2

Query: 257 TGYPLAINATDIKVKEVDFNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSLENK--YD 430
           +G+PL I A ++  KEVDFNP+FI  +  K++W+ L   A S+G+++ LP    +     
Sbjct: 18  SGFPLRIEAGNVIEKEVDFNPDFIRHMFAKIEWKALVEGARSMGYAE-LPEESPDAAVLK 76

Query: 431 ENEEFLKKAHKXXXXXXXXXGHLTCPNLEDNF 526
            +E FLKK H          G L CP     F
Sbjct: 77  SDEPFLKKLHHALLELHLEEGALVCPETGRKF 108



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 15/22 (68%), Positives = 21/22 (95%)
 Frame = +1

Query: 508 KSGRQFPISKGIPNMLLNEAEV 573
           ++GR+FP++KGIPNMLL+E EV
Sbjct: 103 ETGRKFPVNKGIPNMLLHEDEV 124


>At1g78190.1 68414.m09111 expressed protein
          Length = 124

 Score = 58.0 bits (134), Expect = 6e-09
 Identities = 32/94 (34%), Positives = 48/94 (51%), Gaps = 2/94 (2%)
 Frame = +2

Query: 263 YPLAINATDIKVKEVDFNPEFISRVIPKLDWEVLWVAADSIGHSDGLPRSLEN--KYDEN 436
           +PL I A  + VKEVDFNP+F+  +  K+DW+ L   A S+ +++ LP +  +    + +
Sbjct: 20  FPLRIEAEKVTVKEVDFNPDFLRYMFAKIDWKALVDGARSMEYTE-LPDNAPDTTTLESD 78

Query: 437 EEFLKKAHKXXXXXXXXXGHLTCPNLEDNFRFLK 538
           E FL+K H          G L CP     F   K
Sbjct: 79  ETFLRKFHHALLELHLEEGSLVCPETGRKFSVSK 112



 Score = 36.3 bits (80), Expect = 0.022
 Identities = 15/22 (68%), Positives = 20/22 (90%)
 Frame = +1

Query: 508 KSGRQFPISKGIPNMLLNEAEV 573
           ++GR+F +SKGIPNMLL+E EV
Sbjct: 103 ETGRKFSVSKGIPNMLLHEDEV 124


>At2g37840.2 68415.m04646 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 596

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 15/50 (30%), Positives = 28/50 (56%)
 Frame = -1

Query: 501 VKCPSSTSTSNKTL*AFFKNSSFSSYLFSKDLGRPSLWPMLSAATHNTSQ 352
           V  PSS+S+S+K     FK+ S    LF++ +   +  P++ A +++  Q
Sbjct: 290 VDMPSSSSSSSKPYNFPFKSQSPPVELFNRSISSTAPMPIIGATSNSIGQ 339


>At2g37840.1 68415.m04645 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 733

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 15/50 (30%), Positives = 28/50 (56%)
 Frame = -1

Query: 501 VKCPSSTSTSNKTL*AFFKNSSFSSYLFSKDLGRPSLWPMLSAATHNTSQ 352
           V  PSS+S+S+K     FK+ S    LF++ +   +  P++ A +++  Q
Sbjct: 427 VDMPSSSSSSSKPYNFPFKSQSPPVELFNRSISSTAPMPIIGATSNSIGQ 476


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,593,339
Number of Sequences: 28952
Number of extensions: 282365
Number of successful extensions: 632
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 620
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 630
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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