BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30682.Seq (797 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 109 6e-25 SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 42 1e-04 SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizo... 38 0.002 SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schi... 37 0.003 SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schiz... 35 0.012 SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 32 0.11 SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 31 0.19 SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schiz... 31 0.19 SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 30 0.33 SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 30 0.44 SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pomb... 30 0.44 SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 1.0 SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 1.0 SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.3 SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.8 SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 3.1 SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit ... 27 4.1 SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomy... 26 5.4 SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.2 SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces po... 26 7.2 SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 26 7.2 SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 7.2 SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.2 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 7.2 SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 25 9.5 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 109 bits (261), Expect = 6e-25 Identities = 64/188 (34%), Positives = 91/188 (48%) Frame = +1 Query: 223 LAQGQTPQGNRCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 402 + +G+ + IVL+D+ D R+ D+V+I PCP +KY +R+ +L Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137 Query: 403 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHRDDTFRSAGACAPSSSKWSKQIHHHFASWL 582 P+ D+VEGLTG+LF+VYLKPYF+EAYRPI + D F G+ K F Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVS 197 Query: 583 LIP*YTVTVNRLNVRKKRKH*MLSGYDDIGGCRKQLGAN*GDGGVATASSFTVQGYWSEA 762 +N + GYDDIGGCR+Q+ + + + Sbjct: 198 QDTIIHWEGEPINREDEESSLAEVGYDDIGGCRRQMAQIRELVELPLRHPQLFKSIGIKP 257 Query: 763 PRGILMYG 786 PRGILMYG Sbjct: 258 PRGILMYG 265 Score = 88.6 bits (210), Expect = 9e-19 Identities = 41/68 (60%), Positives = 53/68 (77%) Frame = +2 Query: 53 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 232 N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80 Query: 233 GKRRKETV 256 GKRRK+TV Sbjct: 81 GKRRKDTV 88 Score = 64.9 bits (151), Expect = 1e-11 Identities = 30/49 (61%), Positives = 36/49 (73%) Frame = +3 Query: 510 VRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEEEALNAVG 656 VRG MR VEFKVV+ P F IV+ DT+IH +GEPI RE+EE +L VG Sbjct: 174 VRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGEPINREDEESSLAEVG 222 Score = 50.8 bits (116), Expect = 2e-07 Identities = 22/35 (62%), Positives = 26/35 (74%) Frame = +2 Query: 692 AQIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 AQI+E+VELPLRHP LFK+IG+K GPPG Sbjct: 234 AQIRELVELPLRHPQLFKSIGIKPPRGILMYGPPG 268 >SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|Schizosaccharomyces pombe|chr 2|||Manual Length = 403 Score = 41.5 bits (93), Expect = 1e-04 Identities = 15/34 (44%), Positives = 24/34 (70%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +IKE++ELP++HP LF+++G+ GPPG Sbjct: 156 EIKEVIELPVKHPELFESLGIPQPKGILLYGPPG 189 >SPCC1682.16 |rpt4||19S proteasome regulatory subunit Rpt4|Schizosaccharomyces pombe|chr 3|||Manual Length = 388 Score = 37.9 bits (84), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +++E++ELPL++P LF +G+K GPPG Sbjct: 143 ELREVIELPLKNPELFLRVGIKPPKGVLLYGPPG 176 >SPBC4.07c |rpt2|mts2|19S proteasome regulatory subunit Rpt2|Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 37.1 bits (82), Expect = 0.003 Identities = 16/34 (47%), Positives = 21/34 (61%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +IKE VELPL HP L++ +G+K G PG Sbjct: 202 EIKEAVELPLTHPELYEEMGIKPPKGVILYGAPG 235 >SPBC56F2.07c |||AAA family ATPase, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 809 Score = 35.1 bits (77), Expect = 0.012 Identities = 15/35 (42%), Positives = 21/35 (60%) Frame = +2 Query: 692 AQIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 AQI+++VELP ++P LFK + GPPG Sbjct: 289 AQIRDIVELPFQNPELFKFFNIMPPRGVLLYGPPG 323 Score = 31.5 bits (68), Expect = 0.14 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 ++KE VE PL H F +GV+ GPPG Sbjct: 559 KLKESVEWPLTHGETFSRLGVRPPKGVLLYGPPG 592 >SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1190 Score = 31.9 bits (69), Expect = 0.11 Identities = 14/34 (41%), Positives = 20/34 (58%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 Q+KEMV LPL +P +F+ ++ GPPG Sbjct: 277 QLKEMVMLPLLYPEIFQRFNMQPPRGVLFHGPPG 310 >SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizosaccharomyces pombe|chr 3|||Manual Length = 389 Score = 31.1 bits (67), Expect = 0.19 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +++E VELPL L++ IG+ GPPG Sbjct: 145 EVREAVELPLTQGDLYRQIGIDPPRGVLLYGPPG 178 >SPBC16C6.07c |rpt1||19S proteasome regulatory subunit Rpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 438 Score = 31.1 bits (67), Expect = 0.19 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +++E+VELPL P F +G+ GPPG Sbjct: 190 RLREVVELPLLSPERFVKLGIDPPKGIMLYGPPG 223 >SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 641 Score = 30.3 bits (65), Expect = 0.33 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%) Frame = -1 Query: 548 DHFELDGAHAPAD-LKVSSRWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 384 DH L +H PA+ LK I A++ G T +PV P+ ++G + TRF Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410 >SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 1201 Score = 29.9 bits (64), Expect = 0.44 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 Q+KEMV LPL +P +F + + GPPG Sbjct: 383 QLKEMVMLPLLYPEVFLHLHITPPRGVLFHGPPG 416 >SPBC16E9.10c |||AAA family ATPase Rix7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 779 Score = 29.9 bits (64), Expect = 0.44 Identities = 10/34 (29%), Positives = 20/34 (58%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 ++ E+V +P++HP +++ G+ GPPG Sbjct: 185 ELLELVAMPIKHPEVYQYTGIHPPRGVLLHGPPG 218 Score = 27.5 bits (58), Expect = 2.4 Identities = 8/34 (23%), Positives = 19/34 (55%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +++ + P++ P L++++G+ GPPG Sbjct: 503 ELQMAIVQPIKRPELYQSVGISAPTGVLLWGPPG 536 >SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S15a|Schizosaccharomyces pombe|chr 1|||Manual Length = 130 Score = 28.7 bits (61), Expect = 1.0 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%) Frame = -2 Query: 421 QLNHQLAVCGLV---FHISLKDTERWI 350 QLN ++ CG++ F++ LKD E+W+ Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90 >SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schizosaccharomyces pombe|chr 1|||Manual Length = 319 Score = 28.3 bits (60), Expect = 1.3 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%) Frame = +3 Query: 78 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 191 L + R + DP ++ + Q + T+SWH FH +W Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297 >SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosaccharomyces pombe|chr 1|||Manual Length = 905 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 391 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 281 LV + LKD WI + + E F G ++FS+QG Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472 >SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 382 Score = 27.1 bits (57), Expect = 3.1 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +3 Query: 27 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 200 F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342 Query: 201 NS 206 S Sbjct: 343 RS 344 >SPAC3A11.12c |rpt5|pam2, tbp1|19S proteasome regulatory subunit Rpt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 438 Score = 26.6 bits (56), Expect = 4.1 Identities = 11/34 (32%), Positives = 18/34 (52%) Frame = +2 Query: 695 QIKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 ++ E + LP++ F+ +GVK GPPG Sbjct: 196 ELFEAIVLPMQQADKFRKLGVKPPKGCLMFGPPG 229 >SPBP4G3.03 |||PI31 proteasome regulator related|Schizosaccharomyces pombe|chr 2|||Manual Length = 241 Score = 26.2 bits (55), Expect = 5.4 Identities = 14/48 (29%), Positives = 21/48 (43%) Frame = -2 Query: 391 LVFHISLKDTERWIPHQKDAHEGCFSQHGSSEFSHQGNYRLRARCNGF 248 L FH ++ D W PH+ + G F H + +G + R GF Sbjct: 177 LGFHPNVGDYFDWYPHRPNHFGGKFDGHSRGRGAGRGGFCGRHGRGGF 224 >SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +3 Query: 99 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRAN 239 SSV S S ++M++ + +F+ S+ +SSV T + S +N Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSN 375 >SPAC2G11.06 |vps4||AAA family ATPase Vps4|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = +2 Query: 698 IKEMVELPLRHPSLFKAIGVKLHAAFSCMGPPG 796 +KE V LP++ P LF + G K + GPPG Sbjct: 141 LKETVLLPIKLPQLF-SHGRKPWSGILLYGPPG 172 >SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein S0B|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 25.8 bits (54), Expect = 7.2 Identities = 12/25 (48%), Positives = 16/25 (64%) Frame = +3 Query: 576 VAPDTVIHCDGEPIKREEEEEALNA 650 V PD + D E I+REEE++A A Sbjct: 200 VMPDLYFYRDPEEIEREEEQKAAAA 224 >SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 25.8 bits (54), Expect = 7.2 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%) Frame = +2 Query: 77 PD-DLSTAILRRKDRPNRLIVEEAVSD 154 PD D + + + RK+ PNRL++ A+S+ Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259 >SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1275 Score = 25.8 bits (54), Expect = 7.2 Identities = 14/53 (26%), Positives = 30/53 (56%) Frame = +2 Query: 50 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 208 L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+ Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 25.8 bits (54), Expect = 7.2 Identities = 11/21 (52%), Positives = 14/21 (66%) Frame = +1 Query: 607 VNRLNVRKKRKH*MLSGYDDI 669 +N VR++R H L GYDDI Sbjct: 437 INGKYVRRERPHDQLIGYDDI 457 >SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces pombe|chr 3|||Manual Length = 1008 Score = 25.4 bits (53), Expect = 9.5 Identities = 10/38 (26%), Positives = 22/38 (57%) Frame = +2 Query: 137 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRRKE 250 E+AV D+N+ ++L+ + + RGD ++ G ++ Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERD 284 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,343,006 Number of Sequences: 5004 Number of extensions: 67614 Number of successful extensions: 220 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 206 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 218 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 389395636 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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