BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30677.Seq (709 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 95 1e-20 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 95 1e-20 SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces ... 32 0.070 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 29 0.49 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 27 3.5 SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 6.1 SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.0 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 94.7 bits (225), Expect = 1e-20 Identities = 42/59 (71%), Positives = 50/59 (84%) Frame = +2 Query: 80 KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRK 256 KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++K Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKK 75 Score = 80.2 bits (189), Expect = 3e-16 Identities = 59/161 (36%), Positives = 84/161 (52%), Gaps = 2/161 (1%) Frame = +1 Query: 31 MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSA-NPLRSFTW-WKY*VPCA 204 MGI+RD HKR ATG + + ++ + + + P + +R K+ Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 205 ASGHR*LLLGIGMFNSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYT 384 SG+ G + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY Sbjct: 61 DSGN--FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYG 118 Query: 385 LPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVE 507 + +G KG K T +S+ RK+ AR +KV+ Sbjct: 119 ILMG-SKGKKATSTP-----NPKSKHVQRKHSARLGDSKVD 153 Score = 55.6 bits (128), Expect = 7e-09 Identities = 26/35 (74%), Positives = 28/35 (80%) Frame = +3 Query: 510 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKEL 614 ALE QF GRL A V+SRPGQ GR DGYILEG+EL Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEEL 189 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 94.7 bits (225), Expect = 1e-20 Identities = 42/59 (71%), Positives = 50/59 (84%) Frame = +2 Query: 80 KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRK 256 KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++K Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKK 75 Score = 79.4 bits (187), Expect = 5e-16 Identities = 59/161 (36%), Positives = 84/161 (52%), Gaps = 2/161 (1%) Frame = +1 Query: 31 MGISRDHWHKRRATGWETCAHTQEEEV*VRASRCKHQARPSA-NPLRSFTW-WKY*VPCA 204 MGI+RD HKR ATG + + ++ + + + P + +R K+ Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60 Query: 205 ASGHR*LLLGIGMFNSQTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYT 384 SG+ G + +TRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY Sbjct: 61 DSGN--FSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYG 118 Query: 385 LPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVE 507 + +G KG K T +S+ RK+ AR +KV+ Sbjct: 119 ILMG-SKGKKATATP-----TPKSKHVQRKHSARLGDSKVD 153 Score = 55.6 bits (128), Expect = 7e-09 Identities = 26/35 (74%), Positives = 28/35 (80%) Frame = +3 Query: 510 ALEEQFHTGRLLACVASRPGQCGRADGYILEGKEL 614 ALE QF GRL A V+SRPGQ GR DGYILEG+EL Sbjct: 155 ALETQFAAGRLYAVVSSRPGQSGRCDGYILEGEEL 189 >SPAC6G9.02c |nop9||RNA-binding protein Nop9|Schizosaccharomyces pombe|chr 1|||Manual Length = 655 Score = 32.3 bits (70), Expect = 0.070 Identities = 15/34 (44%), Positives = 19/34 (55%) Frame = -2 Query: 606 CLLRCNHRRDHTDLGDSPRKPANALCGIALLEHL 505 CLLR H+RD + + RK N L G LLE + Sbjct: 440 CLLRSKHKRDKANPENKERKLVNNLLGAQLLEEM 473 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 29.5 bits (63), Expect = 0.49 Identities = 16/50 (32%), Positives = 25/50 (50%) Frame = +2 Query: 59 SEGPPAGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 208 S+G K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 35 SKGVKTNKKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 26.6 bits (56), Expect = 3.5 Identities = 16/49 (32%), Positives = 23/49 (46%) Frame = +2 Query: 113 ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKP 259 EL +N + Q+ + S T YRA+ LDTG+ E + KP Sbjct: 1108 ELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDTGDLMAVKEVALHKP 1156 >SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -3 Query: 368 YHCLNGVASTTTIAFLTRVFVRTNS 294 ++C N V S T + + VFVR NS Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542 >SPCC1322.09 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 455 Score = 25.4 bits (53), Expect = 8.0 Identities = 27/92 (29%), Positives = 40/92 (43%), Gaps = 11/92 (11%) Frame = -2 Query: 285 CIIHNINDTGLRVEHSDPQEKLPVS-RRSA---RYL-------VFPPRERTEWIR*GPSL 139 C + ++NDT +E S + LP+ +R A RY+ + P TEW+ S+ Sbjct: 348 CFLIHLNDTNHNLEFSTTSQPLPLEWQRWAVDPRYIKLFGSNAILPNEWVTEWVE--QSI 405 Query: 138 VFAAGRPNSYFLFLRMGARFPAGGPSLMPVIP 43 AAG + RM G PSL +P Sbjct: 406 SVAAGIVAQMYTSKRMA----LGDPSLFAGLP 433 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,717,491 Number of Sequences: 5004 Number of extensions: 54736 Number of successful extensions: 152 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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