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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30677.Seq
         (709 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_39807| Best HMM Match : Ribosomal_S8e (HMM E-Value=0)              128   3e-30
SB_22712| Best HMM Match : Ribosomal_S8e (HMM E-Value=0.068)           36   0.032
SB_43663| Best HMM Match : F-box (HMM E-Value=4e-07)                   27   1.5  
SB_32934| Best HMM Match : K_tetra (HMM E-Value=7.79963e-42)           30   2.1  
SB_26394| Best HMM Match : Plasmodium_HRP (HMM E-Value=0.88)           30   2.1  
SB_51949| Best HMM Match : Toxin_14 (HMM E-Value=0.051)                28   6.5  
SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)                       28   8.5  

>SB_39807| Best HMM Match : Ribosomal_S8e (HMM E-Value=0)
          Length = 192

 Score =  128 bits (310), Expect = 3e-30
 Identities = 69/136 (50%), Positives = 90/136 (66%), Gaps = 2/136 (1%)
 Frame = +1

Query: 253 QTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEE 432
           + RIIDVVYNASNNELVRTKTLVKN IV VD+TPFRQWYE+HY +P+GRKK  + TE E+
Sbjct: 59  KARIIDVVYNASNNELVRTKTLVKNCIVQVDSTPFRQWYEAHYAIPIGRKKTKQPTEEEQ 118

Query: 433 AIINKKRSQKTARKYLARQRLAKVEVL*KSNSTQGVCW--LAWRVAQVSVVAPMVTS*KA 606
            I+NKKRS    RK  AR+  AKV    +     G  +  ++ R  Q       +   + 
Sbjct: 119 EILNKKRSNHCTRKLEARKANAKVAPGMEEQFVTGRLYACVSSRPGQSGRCDGYIL--EG 176

Query: 607 KNFEFYLRKIKSKRAK 654
           K  EFY++K+K+++AK
Sbjct: 177 KELEFYIKKLKARKAK 192



 Score = 93.1 bits (221), Expect = 2e-19
 Identities = 40/56 (71%), Positives = 47/56 (83%)
 Frame = +2

Query: 89  PIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRK 256
           P+R KRK+ELGRP ANT++G +RIH VR+RGGN K+RA RLDT NFSWGSE  TRK
Sbjct: 4   PLRHKRKFELGRPPANTKIGTKRIHEVRTRGGNRKFRAFRLDTENFSWGSESCTRK 59


>SB_22712| Best HMM Match : Ribosomal_S8e (HMM E-Value=0.068)
          Length = 147

 Score = 35.9 bits (79), Expect = 0.032
 Identities = 13/39 (33%), Positives = 24/39 (61%)
 Frame = +1

Query: 262 IIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESH 378
           I+ V+++ +N E +    +VK +IV VD  PF  W++ +
Sbjct: 75  ILSVMHSFANKEHIERNVIVKGSIVQVDNKPFEDWFQEY 113



 Score = 28.7 bits (61), Expect = 4.9
 Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
 Frame = +2

Query: 149 PQRIHSVRSRGGNTKYRALRLDTGNFSWGSE-CSTRKPVSLMLCIMHLTMN 298
           P++I   ++ GG+TK +ALR++ G ++  S+      P+   L +MH   N
Sbjct: 37  PEKIKERKAYGGHTKIKALRVNKGVYTLKSQGVEVEAPI---LSVMHSFAN 84


>SB_43663| Best HMM Match : F-box (HMM E-Value=4e-07)
          Length = 717

 Score = 27.1 bits (57), Expect(2) = 1.5
 Identities = 11/21 (52%), Positives = 16/21 (76%)
 Frame = -2

Query: 501 LSKTLPCQVFSCCLLTSLLVD 439
           + K+LP  + SC LLTSL++D
Sbjct: 523 IGKSLPLLINSCTLLTSLVLD 543



 Score = 21.8 bits (44), Expect(2) = 1.5
 Identities = 8/12 (66%), Positives = 9/12 (75%)
 Frame = -2

Query: 624 IELEVLCLLRCN 589
           + LE LCL RCN
Sbjct: 509 LRLESLCLRRCN 520


>SB_32934| Best HMM Match : K_tetra (HMM E-Value=7.79963e-42)
          Length = 1207

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 21/96 (21%), Positives = 35/96 (36%), Gaps = 2/96 (2%)
 Frame = -3

Query: 377 CDSYHCLNGVASTTTIAFLTRVFVRTNSLL--DALYTTSMIRVCELNIPIPKRSYRCPDA 204
           C  Y C   V +TT +A +  ++++  S +   A+      R C  N             
Sbjct: 700 CPGYKCATPVDNTTILALVPELYLKYTSQVRQKAVEANPSWRWCPGNKCSLVNKVTKRSC 759

Query: 203 AHGT*YFHHVNERSGFAEGRAWCLQRDALTHTSSSC 96
            HG  +  +       A G+ WC +    TH  + C
Sbjct: 760 YHGNTFLDYGGVPVACACGKTWCSECQEETHWPAKC 795


>SB_26394| Best HMM Match : Plasmodium_HRP (HMM E-Value=0.88)
          Length = 842

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +2

Query: 521 AIPHRAFAGLRGESPRSVWSRR 586
           A PH A +G+R  SPR +W  R
Sbjct: 108 ACPHHAVSGVRVSSPRGIWCSR 129



 Score = 29.9 bits (64), Expect = 2.1
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = +2

Query: 521 AIPHRAFAGLRGESPRSVWSRRWL 592
           A PH A +G+R   PRS+W  R L
Sbjct: 139 AYPHHAVSGVRVSPPRSIWCSRVL 162



 Score = 29.9 bits (64), Expect = 2.1
 Identities = 12/24 (50%), Positives = 15/24 (62%)
 Frame = +2

Query: 521 AIPHRAFAGLRGESPRSVWSRRWL 592
           A PH A +G+R   PRS+W  R L
Sbjct: 201 ACPHHAVSGVRVSPPRSIWCSRVL 224



 Score = 29.5 bits (63), Expect = 2.8
 Identities = 11/22 (50%), Positives = 14/22 (63%)
 Frame = +2

Query: 521 AIPHRAFAGLRGESPRSVWSRR 586
           A PH A +G+R   PRS+W  R
Sbjct: 232 ACPHHAVSGVRVSPPRSIWCSR 253


>SB_51949| Best HMM Match : Toxin_14 (HMM E-Value=0.051)
          Length = 387

 Score = 28.3 bits (60), Expect = 6.5
 Identities = 12/38 (31%), Positives = 20/38 (52%)
 Frame = -2

Query: 273 NINDTGLRVEHSDPQEKLPVSRRSARYLVFPPRERTEW 160
           ++ D  +RV+H +  EK  +S      + F PR +T W
Sbjct: 114 SLRDFSIRVKHRNRMEKSTLSEFLETRVCFTPRRKTLW 151


>SB_45647| Best HMM Match : HSP70 (HMM E-Value=0)
          Length = 1327

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 14/52 (26%), Positives = 26/52 (50%)
 Frame = +1

Query: 355  FRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEV 510
            F Q    H+     +KKG  + +   A+   +R  + A++ L+ Q  A+VE+
Sbjct: 930  FDQNVMEHFIKLYKKKKGKNIRKDNRAVQKLRREVEKAKRALSTQHQARVEI 981


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,108,915
Number of Sequences: 59808
Number of extensions: 448920
Number of successful extensions: 1279
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1276
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1865706635
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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