Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30663.Seq
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g80460.1 68414.m09423 glycerol kinase, putative similar to gl... 48 5e-06
At4g30310.3 68417.m04309 ribitol kinase, putative similar to rib... 35 0.066
At4g30310.2 68417.m04308 ribitol kinase, putative similar to rib... 35 0.066
At5g54650.2 68418.m06805 formin homology 2 domain-containing pro... 32 0.35
At5g54650.1 68418.m06804 formin homology 2 domain-containing pro... 32 0.35
At4g24900.1 68417.m03564 expressed protein 30 1.9
At3g27060.1 68416.m03385 ribonucleoside-diphosphate reductase sm... 30 1.9
At1g70620.2 68414.m08137 cyclin-related contains weak similarity... 30 1.9
At5g52040.2 68418.m06459 arginine/serine-rich splicing factor RS... 29 4.3
At5g52040.1 68418.m06458 arginine/serine-rich splicing factor RS... 29 4.3
At2g40070.1 68415.m04923 expressed protein 29 4.3
At1g74260.1 68414.m08600 AIR synthase-related family protein con... 29 4.3
At1g27750.1 68414.m03391 ubiquitin system component Cue domain-c... 29 4.3
At5g27420.1 68418.m03273 zinc finger (C3HC4-type RING finger) fa... 28 5.7
At3g02830.1 68416.m00275 zinc finger (CCCH-type) family protein ... 28 5.7
At1g10520.1 68414.m01184 DNA polymerase lambda (POLL) identical ... 28 5.7
At5g24480.1 68418.m02885 hypothetical protein similar to unknown... 28 7.6
At3g14130.1 68416.m01787 (S)-2-hydroxy-acid oxidase, peroxisomal... 28 7.6
At4g39030.1 68417.m05528 enhanced disease susceptibility 5 (EDS5... 27 10.0
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 10.0
>At1g80460.1 68414.m09423 glycerol kinase, putative similar to
glycerol kinase (ATP:glycerol 3-phosphotransferase,
Glycerokinase, GK)[Mycobacterium tuberculosis]
Swiss-Prot:O69664
Length = 522
Score = 48.4 bits (110), Expect = 5e-06
Identities = 21/36 (58%), Positives = 29/36 (80%)
Frame = -3
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVW 402
+Q+QADL+G PV+RP+ +E+TALGAA AG A+ W
Sbjct: 438 MQIQADLMGSPVVRPVDIETTALGAAYAAGLAVGFW 473
Score = 35.9 bits (79), Expect = 0.029
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -2
Query: 702 PYWRKDARGVMCGLSERTRRQHIVR 628
P+WR+DARGV G++ T + HI R
Sbjct: 365 PWWREDARGVCIGITRFTNKSHIAR 389
Score = 28.3 bits (60), Expect = 5.7
Identities = 15/49 (30%), Positives = 26/49 (53%)
Frame = -1
Query: 739 ISFVPAFNGLYSPILEKRRQGSDVRFERKDSTSAYCQGPRWEAVCHQTR 593
+ FVPAFNGL++P + +G + R + S + E++C Q +
Sbjct: 353 VYFVPAFNGLFAPWWREDARGVCIGITRFTNKSHIARAV-LESMCFQVK 400
>At4g30310.3 68417.m04309 ribitol kinase, putative similar to
ribitol kinase [Klebsiella pneumoniae]
gi|2905643|gb|AAC26495
Length = 451
Score = 34.7 bits (76), Expect = 0.066
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -3
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPT 396
+Q AD++G P+I P ES LGAAI+ A + +P+
Sbjct: 361 IQEHADIVGCPIILPRESESVLLGAAILGAVAGKNYPS 398
>At4g30310.2 68417.m04308 ribitol kinase, putative similar to
ribitol kinase [Klebsiella pneumoniae]
gi|2905643|gb|AAC26495
Length = 579
Score = 34.7 bits (76), Expect = 0.066
Identities = 16/38 (42%), Positives = 24/38 (63%)
Frame = -3
Query: 509 VQMQADLLGIPVIRPLMMESTALGAAIVAGRAMRVWPT 396
+Q AD++G P+I P ES LGAAI+ A + +P+
Sbjct: 489 IQEHADIVGCPIILPRESESVLLGAAILGAVAGKNYPS 526
>At5g54650.2 68418.m06805 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 32.3 bits (70), Expect = 0.35
Identities = 17/39 (43%), Positives = 24/39 (61%), Gaps = 3/39 (7%)
Frame = +1
Query: 514 LSSAPSLRRRGVAS---GARSLQPSRPPRRASGDTRPPS 621
LSS + RR +A+ + S PSRPP+R+ G RPP+
Sbjct: 127 LSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPT 165
>At5g54650.1 68418.m06804 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 900
Score = 32.3 bits (70), Expect = 0.35
Identities = 17/39 (43%), Positives = 24/39 (61%), Gaps = 3/39 (7%)
Frame = +1
Query: 514 LSSAPSLRRRGVAS---GARSLQPSRPPRRASGDTRPPS 621
LSS + RR +A+ + S PSRPP+R+ G RPP+
Sbjct: 127 LSSMLDIPRRNLATKPGSSPSPSPSRPPKRSRGPPRPPT 165
>At4g24900.1 68417.m03564 expressed protein
Length = 421
Score = 29.9 bits (64), Expect = 1.9
Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = -2
Query: 510 CADAG*FTGYTSHSSPHDGK-YSPGGGHCRRSRNACVAHDNTQPTGRYIPAG 358
C ++G G+ HS P K YS G +C + N V+ D Q G Y P G
Sbjct: 224 CDESG--NGFGEHSIPCRSKDYSGNGNYCTQE-NYQVSQDKKQIDGSYNPPG 272
>At3g27060.1 68416.m03385 ribonucleoside-diphosphate reductase small
chain, putative / ribonucleotide reductase, putative
similar to ribonucleotide reductase R2 [Nicotiana
tabacum] GI:1044912; contains Pfam profile PF00268:
Ribonucleotide reductase, small chain
Length = 332
Score = 29.9 bits (64), Expect = 1.9
Identities = 9/31 (29%), Positives = 16/31 (51%)
Frame = -2
Query: 663 LSERTRRQHIVRGRAGRPCVTRRAPWRTRWL 571
+ + R H+ R PCV ++A W +W+
Sbjct: 122 IKDNKERDHLFRAIETIPCVAKKAQWAMKWI 152
>At1g70620.2 68414.m08137 cyclin-related contains weak similarity to
Swiss-Prot:P35662 cylicin I (Multiple-band polypeptide
I) [Bos taurus]
Length = 884
Score = 29.9 bits (64), Expect = 1.9
Identities = 23/74 (31%), Positives = 30/74 (40%), Gaps = 1/74 (1%)
Frame = +2
Query: 398 WATHALRDLRQWPPPGLYFPS*GDE*LVYPVNQPASAQN*VLRHPSV-GEELPQGRAVCS 574
+ TH +D Q PG+ + + V P QP N + S G LPQ S
Sbjct: 181 YPTHGYQDFHQQAVPGVSYQQ-HQQFPVPPTTQPERYPNYATGNESFPGVGLPQENLPTS 239
Query: 575 HRVRHGARLVTHGL 616
+ RLVTH L
Sbjct: 240 SAIHQQERLVTHKL 253
>At5g52040.2 68418.m06459 arginine/serine-rich splicing factor RSP41
(RSP41) nearly identical to SP|P92966
Arginine/serine-rich splicing factor RSP41 {Arabidopsis
thaliana}
Length = 357
Score = 28.7 bits (61), Expect = 4.3
Identities = 23/82 (28%), Positives = 31/82 (37%)
Frame = +1
Query: 409 RIARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPP 588
R A P R RGR + P K A + + RR VAS +R P
Sbjct: 207 RGASPVAHKRERTSPDYGRGRRSPSPYKRARLSPDYKR-DDRRRERVASPENGAVRNRSP 265
Query: 589 RRASGDTRPPSAAPDNMLTSSP 654
R+ G++R P + SP
Sbjct: 266 RKGRGESRSPPPYEKRRESRSP 287
>At5g52040.1 68418.m06458 arginine/serine-rich splicing factor RSP41
(RSP41) nearly identical to SP|P92966
Arginine/serine-rich splicing factor RSP41 {Arabidopsis
thaliana}
Length = 356
Score = 28.7 bits (61), Expect = 4.3
Identities = 23/82 (28%), Positives = 31/82 (37%)
Frame = +1
Query: 409 RIARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPP 588
R A P R RGR + P K A + + RR VAS +R P
Sbjct: 207 RGASPVAHKRERTSPDYGRGRRSPSPYKRARLSPDYKR-DDRRRERVASPENGAVRNRSP 265
Query: 589 RRASGDTRPPSAAPDNMLTSSP 654
R+ G++R P + SP
Sbjct: 266 RKGRGESRSPPPYEKRRESRSP 287
>At2g40070.1 68415.m04923 expressed protein
Length = 607
Score = 28.7 bits (61), Expect = 4.3
Identities = 22/85 (25%), Positives = 36/85 (42%)
Frame = +1
Query: 415 ARPATMAAPRAVLSIMRGRMTGIPSKSACICTELSSAPSLRRRGVASGARSLQPSRPPRR 594
+RPAT+ + A S + S+ LSS+ RR +SG +P+ P R
Sbjct: 133 SRPATLTSRLANSSTESAARNHLTSRQQTSSPGLSSSSGASRRPSSSGGPGSRPATPTGR 192
Query: 595 ASGDTRPPSAAPDNMLTSSPFAQTA 669
+S T ++ + TS +A
Sbjct: 193 SSTLTANSKSSRPSTPTSRATVSSA 217
>At1g74260.1 68414.m08600 AIR synthase-related family protein
contains Pfam profiles: PF00586 AIR synthase related
protein, N-terminal domain, PF02769 AIR synthase related
protein, C-terminal domain
Length = 1387
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -3
Query: 608 VSPDARRGGRDGCRLRAPEATPRRRRDGAELSSV 507
V+PD + GG DG L A +RR G+ L+ V
Sbjct: 898 VTPDLKLGGDDGILLHVDLAKGKRRLGGSALAQV 931
>At1g27750.1 68414.m03391 ubiquitin system component Cue
domain-containing protein very low similarity to ASC-1
complex subunit P100 [Homo sapiens] GI:12061187; contains
Pfam profile PF02845: CUE domain
Length = 1973
Score = 28.7 bits (61), Expect = 4.3
Identities = 20/69 (28%), Positives = 32/69 (46%), Gaps = 5/69 (7%)
Frame = -1
Query: 388 PAHRPIHSCRLLPMKNEKCGEFVGKKP*INAWDGPILK--MNMST---QKIR*NYCSFAA 224
P P+ S RL PM + CG K+ + W G + K ++ ST Q++ + C +
Sbjct: 899 PPFSPLLSPRLPPMVTQLCGSEASKQNIGHQWQGALSKSGVHYSTIIAQRLESDICKYII 958
Query: 223 GTFFPRNRP 197
G+ P P
Sbjct: 959 GSPEPVQWP 967
>At5g27420.1 68418.m03273 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 zinc finger protein
ATL6 [Arabidopsis thaliana]
gi|4928403|gb|AAD33584.1|AF132016_1[4928403]; contains
Pfam domain, PF00097: Zinc finger, C3HC4 type (RING
finger)
Length = 368
Score = 28.3 bits (60), Expect = 5.7
Identities = 19/50 (38%), Positives = 21/50 (42%), Gaps = 1/50 (2%)
Frame = -2
Query: 498 G*FTGYTSH-SSPHDGKYSPGGGHCRRSRNACVAHDNTQPTGRYIPAGSY 352
G FT Y H + DG +P GG RR NA VA T P Y
Sbjct: 60 GFFTVYIRHCTGAVDGSVTPAGGARRRVTNATVARGLDAETIETFPTFVY 109
>At3g02830.1 68416.m00275 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 397
Score = 28.3 bits (60), Expect = 5.7
Identities = 14/46 (30%), Positives = 17/46 (36%)
Frame = -2
Query: 486 GYTSHSSPHDGKYSPGGGHCRRSRNACVAHDNTQPTGRYIPAGSYQ 349
GY S+ D Y GHC+ H QPT +P Q
Sbjct: 127 GYPLRSNEVDCAYFLRTGHCKFGGTCKFNHPQPQPTNMMVPTSGQQ 172
>At1g10520.1 68414.m01184 DNA polymerase lambda (POLL) identical to
DNA polymerase lambda GI:12053869 from [Arabidopsis
thaliana]
Length = 529
Score = 28.3 bits (60), Expect = 5.7
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -2
Query: 552 GNSSPTEGWRRTQFCADAG*FTGYTSHSSPHDGKYSP 442
G+ + +RT++ DAG F G SHS+ SP
Sbjct: 128 GSEDQSSPQKRTRYSPDAGDFKGVESHSNTQGSPDSP 164
>At5g24480.1 68418.m02885 hypothetical protein similar to unknown
protein (gb|AAD32930.1)
Length = 350
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/39 (35%), Positives = 18/39 (46%), Gaps = 3/39 (7%)
Frame = -3
Query: 470 RPLMMESTALGAAIVAGRAMRVWPTTIPSP---PADTFL 363
RP ++ + R R WP T+PSP A TFL
Sbjct: 10 RPHFQSIHFFDCSLTSDRGQRSWPATLPSPQPLSASTFL 48
>At3g14130.1 68416.m01787 (S)-2-hydroxy-acid oxidase, peroxisomal,
putative / glycolate oxidase, putative / short chain
alpha-hydroxy acid oxidase, putative similar to Chain A,
Glycolate Oxidase (E.C.1.1.3.15) Mutant With Tyr 24
Replaced By Phe (Y24f) gi|999542
Length = 363
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = -3
Query: 539 RRRDGAELSSVQMQADLLGIPVIRPLMMESTAL 441
R R ++S++ M +LG P+ P+M+ TA+
Sbjct: 49 RPRVLVDVSNIDMSTSMLGYPISAPIMIAPTAM 81
>At4g39030.1 68417.m05528 enhanced disease susceptibility 5 (EDS5) /
salicylic acid induction deficient 1 (SID1) identical to
SP|Q945F0; contains Pfam profile PF01554:
Uncharacterized membrane protein family
Length = 543
Score = 27.5 bits (58), Expect = 10.0
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -1
Query: 442 WGRPLSQVAQCVCGPRQYPAHRPIHSCRLL 353
WG PLSQ AQ Y A+R + R L
Sbjct: 371 WGEPLSQTAQSFMPEMLYGANRNLPKARTL 400
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.5 bits (58), Expect = 10.0
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 517 SSAPSLRRRGVASGARSLQPSRPPRRASGDTRPPS 621
S APS RRR + AR + PP R PP+
Sbjct: 303 SPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPA 337
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,290,750
Number of Sequences: 28952
Number of extensions: 368307
Number of successful extensions: 1048
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 968
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1046
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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