Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30660.Seq
(738 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2G11.03c |vps45||vacuolar sorting protein Vps 45|Schizosacch... 95 9e-21
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 41 2e-04
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 29 0.52
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 28 1.6
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 2.1
SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr 1|||... 27 2.1
SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schi... 26 4.9
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 26 4.9
SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|... 26 6.4
SPCC126.09 |||vacuolar membrane zinc transporter |Schizosaccharo... 25 8.5
SPBC725.02 |mpr1|spy1|response regulator phosphotransferase |Sch... 25 8.5
SPCPB16A4.05c |||urease accessory protein UREG |Schizosaccharomy... 25 8.5
>SPAC2G11.03c |vps45||vacuolar sorting protein Vps
45|Schizosaccharomyces pombe|chr 1|||Manual
Length = 558
Score = 95.1 bits (226), Expect = 9e-21
Identities = 41/77 (53%), Positives = 55/77 (71%)
Frame = +3
Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEEEAVREVQE 434
H+KC+ F+RPT + LL ELRDPKY Y +YF+NV+ K+ ++ LAE D+ EAV+ +QE
Sbjct: 63 HLKCVAFLRPTPTTLRLLCEELRDPKYAEYHLYFTNVIPKSFLERLAESDDFEAVKSIQE 122
Query: 435 VFADYLAVDRHLFSFNI 485
F DYL V+ L SFNI
Sbjct: 123 FFLDYLVVNNDLASFNI 139
Score = 39.9 bits (89), Expect = 4e-04
Identities = 16/51 (31%), Positives = 36/51 (70%)
Frame = +1
Query: 61 MNVIQAVKMYITKMTEESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYL 213
M+++ A + Y ++ +E +K++L++++TT IVS +QS +L++++YL
Sbjct: 1 MDLVSASQSYFKRIFQEVSD-LKILLLEEDTTKIVSSCITQSNLLEQQIYL 50
Score = 34.3 bits (75), Expect = 0.018
Identities = 20/73 (27%), Positives = 34/73 (46%)
Frame = +2
Query: 509 WNQQHLQRVSQGXXXXXXXXKRRACVRYEAASEPCARLAEKVRDLLRXEAVLIDN*HPLX 688
W R QG K++ +RY+ S C +LAE+V ++ E+ L + P
Sbjct: 150 WQDGAFHRTHQGIISLLLSLKKKPVIRYDNNSLLCLKLAEEVSYTIQHESQLFNFRKP-- 207
Query: 689 TESCPLLQLADHR 727
++ P+L L D +
Sbjct: 208 -DTAPILLLLDRK 219
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 41.1 bits (92), Expect = 2e-04
Identities = 20/77 (25%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
Frame = +3
Query: 267 IVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAE----CDEEEAVREVQE 434
I F++PT ENI L+ +L Y ++ FS+ +S+A ++ AE + + +V +
Sbjct: 92 IYFVQPTQENIELIIEDLSKGLYESAYVCFSSTISRALLEQFAELASKTNTSHMIHQVYD 151
Query: 435 VFADYLAVDRHLFSFNI 485
+ +Y+ ++ FS +
Sbjct: 152 QYLNYVVLESDFFSLQL 168
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2685
Score = 29.5 bits (63), Expect = 0.52
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -3
Query: 280 RIKTMHFMCSCYPIWHDCLSSRISKLPSVRFP 185
R++ +H + SC+P HD S + K P FP
Sbjct: 430 RLELLHDVLSCFPKKHDSTSRKKPKFPYQYFP 461
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -2
Query: 647 SADRGPSPRDERRVLMLPRSERRLAASE 564
SADR P E++ +PR ++LA+SE
Sbjct: 47 SADRKPHRNSEKKTQGMPRKNQQLASSE 74
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 27.5 bits (58), Expect = 2.1
Identities = 24/87 (27%), Positives = 39/87 (44%)
Frame = -2
Query: 593 RSERRLAASETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSVHSQVVSKNFLDLSDS 414
RS+ +A + ADL+ + A +E N+ + SV ++++ N +
Sbjct: 306 RSKAEVAKLVGKSSFADLQLIDKMANAPKHVVEFLENLSLKNSSVLKKILN-NLALMKKK 364
Query: 413 LLFITFGQSFDVCLRNYITEIYKVNTI 333
L + F SFDV R Y T YK + I
Sbjct: 365 ELNLNFLPSFDVWDREYYTARYKQSLI 391
>SPAC167.05 ||SPAC57A7.01|Usp |Schizosaccharomyces pombe|chr
1|||Manual
Length = 601
Score = 27.5 bits (58), Expect = 2.1
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -2
Query: 590 SERRLAASETGGAPADLEKLVGDAVGSNPTLEASNNIERE 471
S+RR++ S T EK+ S+PT AS+N+ E
Sbjct: 175 SQRRMSDSSTPSTTKSKEKIFSPKALSSPTQGASSNVTPE 214
>SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1107
Score = 26.2 bits (55), Expect = 4.9
Identities = 14/46 (30%), Positives = 23/46 (50%)
Frame = +3
Query: 504 RVGTNSISNEFLKVCWRSSCL*SGEPAFATRQHQNPALVSRRRSAI 641
++G + FLK+C R+ CL + + AF LV + SA+
Sbjct: 216 KIGCLFSLSSFLKICPRNDCLQTVDVAFQFLNVAREDLVGQENSAL 261
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.2 bits (55), Expect = 4.9
Identities = 18/62 (29%), Positives = 33/62 (53%)
Frame = -2
Query: 500 LEASNNIEREQVSVHSQVVSKNFLDLSDSLLFITFGQSFDVCLRNYITEIYKVNTIFRIS 321
LE + + R+++ +H + ++K LDLS L T ++FD + + + + N RIS
Sbjct: 765 LEIKDLLVRKRL-LHEEPMTK--LDLSTLRLIRTDSEAFDTFVSDELNSLCATNAYNRIS 821
Query: 320 QF 315
F
Sbjct: 822 TF 823
>SPBC25H2.09 |||DUF1690 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 162
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +3
Query: 213 IREDRQSCQMG*HEHMKCIVFIRPTSENIALLSRELRDPKYG 338
IR D C EH + P +E A+L+ +L +PK G
Sbjct: 122 IRSDLLKCMS---EHPDKSLICHPLAEKFAILASKLHNPKVG 160
>SPCC126.09 |||vacuolar membrane zinc transporter
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 418
Score = 25.4 bits (53), Expect = 8.5
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = -2
Query: 632 PSPRDERRVLMLPRSERRLAASETGGAPADLEKLVGDAVGS 510
PSP + P S +RL++ E+ G+P+ ++VG GS
Sbjct: 159 PSPSTAQNHRSPPASCKRLSSCESSGSPS--SRVVGSLQGS 197
>SPBC725.02 |mpr1|spy1|response regulator phosphotransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 295
Score = 25.4 bits (53), Expect = 8.5
Identities = 21/70 (30%), Positives = 36/70 (51%)
Frame = -2
Query: 590 SERRLAASETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSVHSQVVSKNFLDLSDSL 411
+ ++LAA+ET GAP E+ V + ++ + + +N SV + V + LSD L
Sbjct: 92 NNQQLAANETAGAPEGTEETVD--ISNSISDDHANAKNLPAASVKALVGAG---VLSDEL 146
Query: 410 LFITFGQSFD 381
I + SF+
Sbjct: 147 SVIAYDMSFE 156
>SPCPB16A4.05c |||urease accessory protein UREG |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 286
Score = 25.4 bits (53), Expect = 8.5
Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = -2
Query: 587 ERRLAASETGGAP-ADLEKLVGDAVGSNPTLEASNNIEREQVSVHSQVVSKNF-LDLSDS 414
E R+ A ETGG P A + + V + + L++ N E V ++ N+ DL+D
Sbjct: 140 EERIRAIETGGCPHAAIREDVSGNLVALEELQSEFNTELLLVESGGDNLAANYSRDLADF 199
Query: 413 LLFI 402
++++
Sbjct: 200 IIYV 203
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,925,904
Number of Sequences: 5004
Number of extensions: 59283
Number of successful extensions: 186
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 186
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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