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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= psV30660.Seq
         (738 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g77140.1 68414.m08986 vacuolar protein sorting protein 45, pu...    80   1e-15
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat...    36   0.021
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE...    35   0.049
At3g15060.1 68416.m01905 Ras-related GTP-binding family protein ...    29   2.4  
At3g53510.1 68416.m05908 ABC transporter family protein breast c...    29   4.3  
At5g14390.1 68418.m01681 expressed protein                             28   5.6  
At5g14380.1 68418.m01680 hydroxyproline-rich glycoprotein family...    28   5.6  
At3g01690.1 68416.m00101 expressed protein                             28   5.6  
At1g74430.1 68414.m08623 myb family transcription factor (MYB95)...    28   5.6  
At4g24760.1 68417.m03545 expressed protein                             28   7.4  
At4g31020.2 68417.m04406 expressed protein                             27   9.8  
At4g31020.1 68417.m04405 expressed protein                             27   9.8  
At3g11900.1 68416.m01459 amino acid transporter family protein l...    27   9.8  
At2g24320.1 68415.m02907 hypothetical protein                          27   9.8  
At1g08440.1 68414.m00933 hypothetical protein contains Pfam prof...    27   9.8  

>At1g77140.1 68414.m08986 vacuolar protein sorting protein 45,
           putative / VPS45p, putative identical to vacuolar
           protein sorting homolog VPS45p [Arabidopsis thaliana]
           gi|2921406|gb|AAC39472
          Length = 569

 Score = 80.2 bits (189), Expect = 1e-15
 Identities = 33/77 (42%), Positives = 55/77 (71%)
 Frame = +3

Query: 255 HMKCIVFIRPTSENIALLSRELRDPKYGVYFIYFSNVVSKADIKTLAECDEEEAVREVQE 434
           H+K + FIRPTS+NI  L  +L +P++G Y ++FSN++    I  LA+ DE+E V++VQE
Sbjct: 66  HLKAVYFIRPTSDNIQKLRYQLANPRFGEYHLFFSNLLKDTQIHILADSDEQEVVQQVQE 125

Query: 435 VFADYLAVDRHLFSFNI 485
            +AD+++ D + F+ N+
Sbjct: 126 YYADFVSGDPYHFTLNM 142



 Score = 63.3 bits (147), Expect = 2e-10
 Identities = 31/57 (54%), Positives = 44/57 (77%)
 Frame = +1

Query: 61  MNVIQAVKMYITKMTEESGPGMKVILMDKETTSIVSMVYSQSEILQKEVYLFERIDS 231
           M ++ +V+ YI +M ++   GMKV+++D ET S VS+VYSQSE+LQKEV+L E IDS
Sbjct: 1   MVLVTSVRDYINRMLQDIS-GMKVLILDSETVSNVSIVYSQSELLQKEVFLVEMIDS 56



 Score = 35.5 bits (78), Expect = 0.037
 Identities = 24/72 (33%), Positives = 34/72 (47%), Gaps = 1/72 (1%)
 Frame = +2

Query: 515 QQHLQRVSQGXXXXXXXXKRRACVRYEAASEPCARLAEKVRDLL-RXEAVLIDN*HPLXT 691
           Q+   RV  G        KRR  +RY+  S+   R+A +   L+ + E+ L D      T
Sbjct: 160 QRFSDRVVDGIAAVFLALKRRPVIRYQRTSDTAKRIAHETAKLMYQHESALFDF---RRT 216

Query: 692 ESCPLLQLADHR 727
           ES PLL + D R
Sbjct: 217 ESSPLLLVIDRR 228


>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 662

 Score = 36.3 bits (80), Expect = 0.021
 Identities = 21/84 (25%), Positives = 45/84 (53%), Gaps = 3/84 (3%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEEEAVREV 428
           M+ I FI+PT EN+     ++  + P Y   F++FS+ VS++ +  +  +    + +  +
Sbjct: 86  MEVIYFIQPTEENVTAFLSDMTGKSPLYKKAFVFFSSPVSRSLVNLIKKDMRAMKRIGGL 145

Query: 429 QEVFADYLAVDRHLFSFNIVGCLQ 500
           +E+  +Y+++D   F  N    L+
Sbjct: 146 KEMNLEYISMDIQGFVTNNENALE 169



 Score = 31.9 bits (69), Expect = 0.46
 Identities = 16/36 (44%), Positives = 21/36 (58%)
 Frame = +1

Query: 127 KVILMDKETTSIVSMVYSQSEILQKEVYLFERIDSH 234
           KV++MDK T  I+S     SEI Q+ + L E I  H
Sbjct: 44  KVLVMDKFTVKIMSSACKMSEITQEGISLVEVITKH 79


>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
           similar to cytokinesis-related Sec1 protein KEULE
           [Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
           Pfam domain, PF00995: Sec1 family
          Length = 666

 Score = 35.1 bits (77), Expect = 0.049
 Identities = 23/95 (24%), Positives = 45/95 (47%), Gaps = 3/95 (3%)
 Frame = +3

Query: 258 MKCIVFIRPTSENIALLSREL--RDPKYGVYFIYFSNVVSKADIKTL-AECDEEEAVREV 428
           M  I FI+PT EN+ +   ++  + P Y   F++FS+ VSK  +  +  +      +  +
Sbjct: 86  MDAIYFIQPTKENVIMFLSDMSGKSPLYKKAFVFFSSPVSKELVGHIKKDSSVLPRIGAL 145

Query: 429 QEVFADYLAVDRHLFSFNIVGCLQGRVGTNSISNE 533
           +E+  ++ A+D   F  +    L+   G    S +
Sbjct: 146 REMNLEFFAIDSQGFITDHERALEDLFGDEETSRK 180


>At3g15060.1 68416.m01905 Ras-related GTP-binding family protein
           similar to GTP-binding protein GI:303742 from [Pisum
           sativum]; contains Pfam profile: PF00071 ras family
          Length = 217

 Score = 29.5 bits (63), Expect = 2.4
 Identities = 27/83 (32%), Positives = 38/83 (45%), Gaps = 2/83 (2%)
 Frame = -2

Query: 584 RRLAASETGGAPADLEKLVGDAVGSNPTLEASN--NIEREQVSVHSQVVSKNFLDLSDSL 411
           R L A  T  A A  E+           LEA N  N   E +S   +V SK  LD+ D  
Sbjct: 131 RHLRAVSTEDAKAFAER-ENTFFMETSALEALNVENAFTEVLSQIYRVASKKALDIGDDH 189

Query: 410 LFITFGQSFDVCLRNYITEIYKV 342
             +  GQS +V  ++ ++E+ KV
Sbjct: 190 TTLPKGQSINVGSKDDVSEVKKV 212


>At3g53510.1 68416.m05908 ABC transporter family protein breast
           cancer resistance protein (BCRP), Homo sapiens,
           EMBL:AF098951
          Length = 739

 Score = 28.7 bits (61), Expect = 4.3
 Identities = 15/49 (30%), Positives = 27/49 (55%)
 Frame = -2

Query: 176 YTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFILIKLSLFISQ 30
           +TILT      S  +TF+ G +S V++   ++ A + + L+    FIS+
Sbjct: 568 FTILTAFWAGSSF-VTFLSGVVSHVMIGFTVVVAILAYFLLFSGFFISR 615


>At5g14390.1 68418.m01681 expressed protein
          Length = 369

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 16/43 (37%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
 Frame = -2

Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441
           ET GA  +   L G +VGS PTL+ +  + + + +V HS ++S
Sbjct: 132 ETYGAKQEDIILYGQSVGSGPTLDLAARLPQLRAAVLHSPILS 174


>At5g14380.1 68418.m01680 hydroxyproline-rich glycoprotein family
           protein identical to gi|4775268|emb|CAB42531
          Length = 150

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = +1

Query: 514 PTASPTSFSRSAGAPPVSEAASLRSLRGSIRTLRSSRGEGP 636
           PT +PT+ +++  AP  + AA+ +S   S     S   EGP
Sbjct: 38  PTKAPTATTKAPSAPTKAPAAAPKSSSASSPKASSPAAEGP 78


>At3g01690.1 68416.m00101 expressed protein
          Length = 361

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 1/43 (2%)
 Frame = -2

Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNI-EREQVSVHSQVVS 441
           ET G+  +   L G +VGS PTL+ ++ + +   V +HS ++S
Sbjct: 132 ETFGSKQEGVILYGQSVGSGPTLDLASRLPQLRAVVLHSPILS 174


>At1g74430.1 68414.m08623 myb family transcription factor (MYB95)
           contains Pfam profile: PF00249 myb-like DNA-binding
           domain
          Length = 271

 Score = 28.3 bits (60), Expect = 5.6
 Identities = 18/53 (33%), Positives = 30/53 (56%)
 Frame = +1

Query: 391 WPNVMKRRLSERSRKFLLTTWLWTDTCSLSILLDASKVGLEPTASPTSFSRSA 549
           W + +K+RL+++    +  T   T T SL++ + +S     PT SPTS S S+
Sbjct: 108 WNSCLKKRLAKKGIDPM--THEPTTTTSLTVDVTSSSTTSSPTPSPTSSSFSS 158


>At4g24760.1 68417.m03545 expressed protein
          Length = 365

 Score = 27.9 bits (59), Expect = 7.4
 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 566 ETGGAPADLEKLVGDAVGSNPTLEASNNIEREQVSV-HSQVVS 441
           E  GA  +   L G +VGS PT++ +  + R + S+ HS ++S
Sbjct: 132 ENYGAKQENIILYGQSVGSGPTVDLAARLPRLRASILHSPILS 174


>At4g31020.2 68417.m04406 expressed protein
          Length = 294

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = -2

Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
           L G +VGS PTL  ++ ++R   V +HS ++S
Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174


>At4g31020.1 68417.m04405 expressed protein
          Length = 294

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/32 (40%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = -2

Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
           L G +VGS PTL  ++ ++R   V +HS ++S
Sbjct: 143 LYGQSVGSGPTLHMASRLKRLRGVVLHSAILS 174


>At3g11900.1 68416.m01459 amino acid transporter family protein low
           similarity to proton/amino acid transporter 1 [Mus
           musculus] GI:21908024; belongs to INTERPRO:IPR002422
           amino acid/polyamine transporter, family II
          Length = 432

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = -2

Query: 179 LYTILTMLVVSLSINITFIPGPLSSVILVMYILTACITFIL 57
           ++T  T+LVV L+   + +PG  +   LV   L A I+F+L
Sbjct: 349 IFTTRTLLVVGLAAIASLVPGFGTFASLVGSTLCALISFVL 389


>At2g24320.1 68415.m02907 hypothetical protein
          Length = 286

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 12/32 (37%), Positives = 21/32 (65%), Gaps = 1/32 (3%)
 Frame = -2

Query: 533 LVGDAVGSNPTLEASNNIER-EQVSVHSQVVS 441
           L G +VGS PTL  ++ ++R   + +HS ++S
Sbjct: 135 LYGQSVGSGPTLHLASRVKRLRGIVLHSAILS 166


>At1g08440.1 68414.m00933 hypothetical protein contains Pfam profile
           PF01027: Uncharacterized protein family UPF0005
          Length = 501

 Score = 27.5 bits (58), Expect = 9.8
 Identities = 13/44 (29%), Positives = 25/44 (56%)
 Frame = +1

Query: 37  INKDNLIKMNVIQAVKMYITKMTEESGPGMKVILMDKETTSIVS 168
           IN D  I M++ + ++  + +M+ ESG  MK + +  +  +I S
Sbjct: 289 INSDMQIPMDIKKKIEEPLRRMSSESGKSMKEVSISLKNMTISS 332


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,197,955
Number of Sequences: 28952
Number of extensions: 305499
Number of successful extensions: 900
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 874
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 899
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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