BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= psV30659.Seq (598 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 85 6e-18 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 60 2e-10 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.7 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 27 2.7 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 4.8 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 25 6.3 SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizo... 25 8.4 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 85.4 bits (202), Expect = 6e-18 Identities = 38/88 (43%), Positives = 58/88 (65%), Gaps = 2/88 (2%) Frame = +1 Query: 1 SVKVHPVVLFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVEA 174 ++ + P VLF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE Sbjct: 22 NIVIEPAVLFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVEV 81 Query: 175 ELNYAMDVYELNRRVNSSESIVGWWATA 258 E+ Y +Y L+ + N E +VGW+AT+ Sbjct: 82 EMEYHRAMYHLHLKANPREVVVGWYATS 109 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 60.5 bits (140), Expect = 2e-10 Identities = 30/88 (34%), Positives = 49/88 (55%), Gaps = 4/88 (4%) Frame = +1 Query: 4 VKVHPVVLFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA-- 174 V VHP+VL VD+Y R RV+G LLG ++ VV V N + +P +E Sbjct: 17 VIVHPLVLLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWF 76 Query: 175 -ELNYAMDVYELNRRVNSSESIVGWWAT 255 + N+ + E+ +++N++E +VGW+ T Sbjct: 77 LDHNFMESMNEMFKKINANEKLVGWYHT 104 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.7 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +1 Query: 46 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 171 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 26.6 bits (56), Expect = 2.7 Identities = 13/44 (29%), Positives = 24/44 (54%) Frame = -3 Query: 428 SEHATLLSIWHSQWYTNICT*THSTSSQRSVQSNMDXLTAFTGI 297 S A +L + W+ N+ T S+RS+ +N D +T+F+ + Sbjct: 410 SAKARILDSFCIDWHLNV-----QTMSERSILTNRDFITSFSAV 448 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 4.8 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +3 Query: 57 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 200 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 25.4 bits (53), Expect = 6.3 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +2 Query: 419 HVHSCRCHSHM 451 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 >SPAC1142.03c |swi2|SPAC17G6.20c|Swi5 complex subunit Swi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 722 Score = 25.0 bits (52), Expect = 8.4 Identities = 17/48 (35%), Positives = 23/48 (47%) Frame = -1 Query: 367 KPIRPPASEVSRVTWTXSRHSRE*YSCITEEWLVTSLPSPTNQQYFQR 224 KP PP S++SR T S S E C + T+ PS T+ + R Sbjct: 566 KPFHPPISKISRTRLTVS--SPERLYCAKPISMATA-PSETDSKLINR 610 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,467,011 Number of Sequences: 5004 Number of extensions: 51584 Number of successful extensions: 150 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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