Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= psV30659.Seq
(598 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g39990.1 68415.m04914 eukaryotic translation initiation facto... 82 2e-16
At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory su... 76 2e-14
At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory su... 75 3e-14
At1g10840.1 68414.m01246 eukaryotic translation initiation facto... 49 2e-06
At5g01240.2 68418.m00032 amino acid permease, putative strong si... 30 1.0
At5g01240.1 68418.m00031 amino acid permease, putative strong si... 30 1.0
At2g04865.1 68415.m00502 expressed protein ; expression supporte... 29 3.1
At5g22940.1 68418.m02682 exostosin family protein contains Pfam ... 28 4.1
At2g46280.3 68415.m05757 eukaryotic translation initiation facto... 28 4.1
At2g46280.2 68415.m05756 eukaryotic translation initiation facto... 28 4.1
At2g46280.1 68415.m05755 eukaryotic translation initiation facto... 28 4.1
At2g28110.1 68415.m03415 exostosin family protein contains 1 tra... 27 9.5
>At2g39990.1 68415.m04914 eukaryotic translation initiation factor 3
subunit 5 / eIF-3 epsilon / eIF3f (TIF3F1) identical to
SP|O04202 Eukaryotic translation initiation factor 3
subunit 5 (eIF-3 epsilon) (eIF3 p32 subunit) (eIF3f)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 293
Score = 82.2 bits (194), Expect = 2e-16
Identities = 35/86 (40%), Positives = 58/86 (67%), Gaps = 1/86 (1%)
Frame = +1
Query: 1 SVKVHPVVLFQIVDAYERRNADSHRVIGTLLGTS-DKGVVEVTNCFCVPHKEHADQVEAE 177
+ ++HP+V+F + D + RR + RVIGTLLG+ G V++ N + VPH E +DQV +
Sbjct: 27 TARIHPLVIFNVCDCFVRRPDSAERVIGTLLGSILPDGTVDIRNSYAVPHNESSDQVAVD 86
Query: 178 LNYAMDVYELNRRVNSSESIVGWWAT 255
++Y ++ + +VNS E+IVGW++T
Sbjct: 87 IDYHHNMLASHLKVNSKETIVGWYST 112
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/80 (31%), Positives = 41/80 (51%)
Frame = +3
Query: 255 GNEVTNHSSVIHEYYSRECREXVHVTLDTSLAGGRMGLRAYVCVPLGVPNGKQGCMFTPV 434
G V SS+IH++Y+RE +H+T+DT G ++A+V L + + + F +
Sbjct: 113 GAGVNGGSSLIHDFYAREVPNPIHLTVDTGFTNGEGTIKAFVSSNLSLGDRQLVAHFQEI 172
Query: 435 DVTLTCYEPEIVGLQVCQKT 494
V L + E VG V + T
Sbjct: 173 PVDLRMVDAERVGFDVLKAT 192
>At5g05780.1 68418.m00636 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26s proteasome regulatory subunit s12
(proteasome subunit p40) (mov34 protein) SP:P26516 from
[Mus musculus]; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 308
Score = 76.2 bits (179), Expect = 2e-14
Identities = 39/91 (42%), Positives = 56/91 (61%), Gaps = 4/91 (4%)
Frame = +1
Query: 4 VKVHPVVLFQIVDAYERRNADSH-RVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE--- 171
V VHP+VL IVD Y R DS RV+G LLG+S +GVV+VTN + VP +E
Sbjct: 17 VVVHPLVLLSIVDHYNRVAKDSSKRVVGVLLGSSSRGVVDVTNSYAVPFEEDDKDPSIWF 76
Query: 172 AELNYAMDVYELNRRVNSSESIVGWWATAMK 264
+ NY ++ + +R+N+ E +VGW++T K
Sbjct: 77 LDHNYHESMFHMFKRINAKEHVVGWYSTGPK 107
>At3g11270.1 68416.m01370 26S proteasome non-ATPase regulatory
subunit 7, putative / 26S proteasome regulatory subunit
S12, putative / MOV34 protein, putative contains
similarity to 26S proteasome regulatory subunit S12
(MOV34) SP:P26516 from [Mus musculus]
Length = 310
Score = 75.4 bits (177), Expect = 3e-14
Identities = 39/91 (42%), Positives = 55/91 (60%), Gaps = 4/91 (4%)
Frame = +1
Query: 4 VKVHPVVLFQIVDAYERRNAD-SHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE--- 171
V VHP+VL IVD Y R D S RV+G LLG+S +G V+VTN + VP +E
Sbjct: 17 VIVHPLVLLSIVDHYNRVAKDTSKRVVGVLLGSSSRGTVDVTNSYAVPFEEDDKDTSIWF 76
Query: 172 AELNYAMDVYELNRRVNSSESIVGWWATAMK 264
+ NY ++ + +R+N+ E IVGW++T K
Sbjct: 77 LDHNYHESMFHMFKRINAKEHIVGWYSTGPK 107
>At1g10840.1 68414.m01246 eukaryotic translation initiation factor 3
subunit 3 / eIF-3 gamma / eIF3h (TIF3H1) identical to
SP|Q9C5Z2 Eukaryotic translation initiation factor 3
subunit 3 (eIF-3 gamma) (eIF3 p38 subunit) (eIF3h)
{Arabidopsis thaliana}; contains Pfam profile PF01398:
Mov34/MPN/PAD-1 family
Length = 337
Score = 49.2 bits (112), Expect = 2e-06
Identities = 23/63 (36%), Positives = 37/63 (58%), Gaps = 1/63 (1%)
Frame = +1
Query: 76 VIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEAE-LNYAMDVYELNRRVNSSESIVGWWA 252
V G LLG V+EVTNCF P ++ +++EA+ NY +++ R VN + VGW+
Sbjct: 47 VTGQLLGLDVGSVLEVTNCFPFPVRDDDEEIEADGANYQLEMMRCLREVNVDNNTVGWYQ 106
Query: 253 TAM 261
+ +
Sbjct: 107 STV 109
>At5g01240.2 68418.m00032 amino acid permease, putative strong
similarity to AUX1 GI:1531758 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 408
Score = 30.3 bits (65), Expect = 1.0
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 219 DSSVQLVNIHRVIKFRFDLIGMFFVWHAEAVGYFH 115
D++V L+ IH+ I F F ++FVW +A+G H
Sbjct: 238 DTAVILMLIHQFITFGFACTPLYFVWE-KAIGMHH 271
>At5g01240.1 68418.m00031 amino acid permease, putative strong
similarity to AUX1 GI:1531758 from [Arabidopsis
thaliana]; contains Pfam profile PF01490: Transmembrane
amino acid transporter protein
Length = 488
Score = 30.3 bits (65), Expect = 1.0
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -2
Query: 219 DSSVQLVNIHRVIKFRFDLIGMFFVWHAEAVGYFH 115
D++V L+ IH+ I F F ++FVW +A+G H
Sbjct: 318 DTAVILMLIHQFITFGFACTPLYFVWE-KAIGMHH 351
>At2g04865.1 68415.m00502 expressed protein ; expression supported
by MPSS
Length = 667
Score = 28.7 bits (61), Expect = 3.1
Identities = 13/36 (36%), Positives = 16/36 (44%)
Frame = +2
Query: 482 VPEDCVWWXRGSSGVAGXSDLAQVMFGRRARWISCW 589
+P D W R S GV G DL+ M + W W
Sbjct: 356 IPADVQRWVRKSRGVDGGVDLSNKMESELSEWEMRW 391
>At5g22940.1 68418.m02682 exostosin family protein contains Pfam
profile: PF03016 exostosin family
Length = 469
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/27 (48%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +2
Query: 341 FAGWRSNGFTCI-CLCTIGSAKWKARL 418
FAG+RS + CLC +G A W RL
Sbjct: 332 FAGYRSEIVRSVFCLCPLGWAPWSPRL 358
>At2g46280.3 68415.m05757 eukaryotic translation initiation factor 3
subunit 2 / TGF-beta receptor interacting protein 1 /
eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) identical to
eukaryotic translation initiation factor 3 subunit 2
(SP:Q38884) {Arabidopsis thaliana}; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies)
Length = 254
Score = 28.3 bits (60), Expect = 4.1
Identities = 21/62 (33%), Positives = 31/62 (50%), Gaps = 5/62 (8%)
Frame = +1
Query: 163 QVEAELNYAMDVYELNRRVNSSESIVGWWATAMK*---PTTPLL--YTSIIPVNAVSXSM 327
Q + E+ + D+ L + + S + G K T LL YT+++PVNAVS S
Sbjct: 185 QSDEEVGHKKDITSLCKAADDSHFLTGSLDKTAKLWDMRTLTLLKTYTTVVPVNAVSLSP 244
Query: 328 LL 333
LL
Sbjct: 245 LL 246
>At2g46280.2 68415.m05756 eukaryotic translation initiation factor 3
subunit 2 / TGF-beta receptor interacting protein 1 /
eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) identical to
eukaryotic translation initiation factor 3 subunit 2
(SP:Q38884) {Arabidopsis thaliana}; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies)
Length = 328
Score = 28.3 bits (60), Expect = 4.1
Identities = 21/62 (33%), Positives = 31/62 (50%), Gaps = 5/62 (8%)
Frame = +1
Query: 163 QVEAELNYAMDVYELNRRVNSSESIVGWWATAMK*---PTTPLL--YTSIIPVNAVSXSM 327
Q + E+ + D+ L + + S + G K T LL YT+++PVNAVS S
Sbjct: 185 QSDEEVGHKKDITSLCKAADDSHFLTGSLDKTAKLWDMRTLTLLKTYTTVVPVNAVSLSP 244
Query: 328 LL 333
LL
Sbjct: 245 LL 246
>At2g46280.1 68415.m05755 eukaryotic translation initiation factor 3
subunit 2 / TGF-beta receptor interacting protein 1 /
eIF-3 beta / eIF3i / TRIP-1 (TIF3I1) identical to
eukaryotic translation initiation factor 3 subunit 2
(SP:Q38884) {Arabidopsis thaliana}; contains Pfam
PF00400: WD domain, G-beta repeat (5 copies)
Length = 328
Score = 28.3 bits (60), Expect = 4.1
Identities = 21/62 (33%), Positives = 31/62 (50%), Gaps = 5/62 (8%)
Frame = +1
Query: 163 QVEAELNYAMDVYELNRRVNSSESIVGWWATAMK*---PTTPLL--YTSIIPVNAVSXSM 327
Q + E+ + D+ L + + S + G K T LL YT+++PVNAVS S
Sbjct: 185 QSDEEVGHKKDITSLCKAADDSHFLTGSLDKTAKLWDMRTLTLLKTYTTVVPVNAVSLSP 244
Query: 328 LL 333
LL
Sbjct: 245 LL 246
>At2g28110.1 68415.m03415 exostosin family protein contains 1
transmembrane domain; similar to
pectin-glucuronyltransferase (GI:23821292) [Nicotiana
plumbaginifolia]; similar to NpGUT1 homolog
(GI:23821294) [Arabidopsis thaliana]; contains Pfam
profile PF03016: Exostosin family
Length = 448
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/27 (44%), Positives = 16/27 (59%), Gaps = 1/27 (3%)
Frame = +2
Query: 341 FAGWRSN-GFTCICLCTIGSAKWKARL 418
FAG++S + CLC +G A W RL
Sbjct: 318 FAGYQSEIARSVFCLCPLGWAPWSPRL 344
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,178,839
Number of Sequences: 28952
Number of extensions: 283966
Number of successful extensions: 686
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 666
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 680
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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